Insights into the retention mechanism on an octadecylsiloxane-bonded silica stationary phase (HyPURITY C18) in reversed-phase liquid chromatography

2006 ◽  
Vol 1115 (1-2) ◽  
pp. 133-141 ◽  
Author(s):  
Colin F. Poole ◽  
Waruna Kiridena ◽  
Colleen DeKay ◽  
Wladyslaw W. Koziol ◽  
Renae D. Rosencrans
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Reversed Phase ◽  
Retention Mechanism ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

scholarly journals Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Nicola Marchetti ◽  
Pier Paolo Giovannini ◽  
Martina Catani ◽  
Luisa Pasti ◽  
Alberto Cavazzini
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Retention Mechanism ◽  
Thermodynamic Quantities ◽  
Reversed Phase Liquid Chromatography ◽  
Mobile Phase Composition ◽  
Solute Transfer ◽  
Phase Liquid

The retention mechanism of four major carotenoids, two xanthophylls (i.e., lutein and zeaxanthin) and two carotenes (i.e., lycopene and β-carotene), was investigated in reversed-phase liquid chromatography with the aim of thermodynamic analysis. The experimental variables considered in this study were the composition of mobile phase (MP) and the temperature. Chromatographic elutions were undertaken under linear, isocratic conditions by using a C18 stationary phase, four different MP compositions (by varying the ratio methanol/acetonitrile from 66.5/28.5 to 47.5/47.5 v/v), and column temperatures in the range 283–313 K. Traditional Van’t Hoff analysis has been used to estimate changes of standard enthalpy (ΔH∘) and Gibbs free energy (ΔG∘) associated with the solute transfer from the mobile to the stationary phase at each mobile phase composition. The thermodynamic quantities have been correlated to the structure of investigated carotenoids and their interaction with the octadecyl silica stationary phase.

Retention Mechanism in Reversed-Phase Liquid Chromatography:  A Molecular Perspective

2007 ◽  
Vol 79 (17) ◽  
pp. 6551-6558 ◽  
Author(s):  
Jake L. Rafferty ◽  
Ling Zhang ◽  
J. Ilja Siepmann ◽  
Mark R. Schure
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Retention Mechanism ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid
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