Erratum to “Kinetic optimisation of the reversed phase liquid chromatographic separation of rooibos tea (Aspalathus linearis) phenolics on conventional high performance liquid chromatographic instrumentation” [J. Chromatogr. A 1219 (2012) 128–139]

2012 ◽  
Vol 1241 ◽  
pp. 128 ◽  
Author(s):  
Theresa Beelders ◽  
Gunnar O. Sigge ◽  
Elizabeth Joubert ◽  
Dalene de Beer ◽  
André de Villiers
Keyword(s):  
Chromatographic Separation ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Aspalathus Linearis ◽  
Rooibos Tea ◽  
Liquid Chromatographic Separation ◽  
Phase Liquid ◽  
Liquid Chromatographic

Reversed-phase liquid chromatographic separation of 3',5'-cyclic ribonucleotides.

1979 ◽  
Vol 25 (2) ◽  
pp. 235-241 ◽  
Author(s):  
A M Krstulovic ◽  
R A Hartwick ◽  
P R Brown
Keyword(s):  
Chromatographic Separation ◽  
High Performance ◽  
Peak Shift ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Separation ◽  
Biological Extracts ◽  
Phase Liquid ◽  
Chromatographic Peaks ◽  
Liquid Chromatographic

Abstract A rapid, reversed-phase "high-performance" liquid-chromatographic separation of the five naturally occurring cyclic ribonucleotides is described. The separation, optimized for the measurement of these compounds in biological samples, is short (25 min), sensitive (50-100 pmol), and requires no sample pre-concentration steps. We also report an alternative isocratic elution mode, optimized for a rapid and selective analysis for adenosine 3',5'-cyclic phosphate. The identity of chromatographic peaks in biological extracts is confirmed by several methods: retention times, co-chromatography with the reference compounds, absorbance ratios, enzymatic peak-shift with cyclic nucleotide phosphodiesterase, and stopped-flow ultraviolet-scanning techniques.

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