Regulation of synthesis of the pectolytic enzymes of Aspergillus niger

2004 ◽  
Vol 34 (5) ◽  
pp. 466-473 ◽  
Author(s):  
T. Panda ◽  
Sushma R. Nair ◽  
M.Prem Kumar
Keyword(s):  
Aspergillus Niger ◽  
Pectolytic Enzymes ◽  
Regulation Of Synthesis

Purification, characterization and mode of effect of another endo-D-galacturonanase from Aspergillus niger

1981 ◽  
Vol 46 (12) ◽  
pp. 3145-3156 ◽  
Author(s):  
Kvetoslava Heinrichová ◽  
Mária Dzúrová
Keyword(s):  
Molecular Weight ◽  
Aspergillus Niger ◽  
Degradation Products ◽  
Maximal Activity ◽  
Kinetic Constants ◽  
Pectic Acid ◽  
Commercial Preparation ◽  
Glycosidic Bonds ◽  
Pectolytic Enzymes ◽  
Approximate Molecular Weight

A mixture of pectolytic enzymes was isolated from a commercial preparation of Pectinex Ultra. endo-D-Galacturonanase (EC 3.2.1.15) was obtained from this mixture of enzymes, produced by Aspergillus niger cultures, by affinity chromatography using cross-linked pectic acid as a support and by subsequent chromatography on a Sephadex G-100 column. One form of endo-D-galacturonanase only of approximate molecular weight of 35 000 was detected in the purified product by electrophoresis in polyacrylamide gel. The enzyme showed maximal activity and stability at pH 4.9 and 40°. The mode of degradation of a high molecular weight substrate and the per cent of the glycosidic bonds cleaved (6%) at a viscosity decrease by 50% indicate an endo type action pattern. endo-D-Galacturonanase was characterized by the mode of cleavage and the kinetic constants Km and V for oligogalacturonic acids and polygalacturonic acid. On the basis of the knowledge of the kinetic constants and the degradation products the mode of action of the enzyme and the extent of its active site are discussed.

Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger

1999 ◽  
Vol 34 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Leda R. Castilho ◽  
Tito L.M. Alves ◽  
Ricardo A. Medronho
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
State Culture ◽  
Solid State Culture ◽  
Pectolytic Enzymes

Concomitant biosynthesis of Aspergillus niger pectolytic enzymes and citric acid on sucrose

1994 ◽  
Vol 16 (8) ◽  
pp. 703-707 ◽  
Author(s):  
Joz̆ica Friedrich ◽  
Aleksa Cimerman ◽  
Walter Steiner
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Pectolytic Enzymes
Sign in / Sign up

Export Citation Format

Share Document