Identification and characterization of the first β-1,3-d-xylosidase from a gram-positive bacterium, Streptomyces sp. SWU10

The trimethoprim-resistance gene located on plasmid pSK1, originally identified in a multi-resistant Staphylococcus aureus from Australia, encodes the production of a dihydrofolate reductase (type S1), which confers a high degree of resistance to its host and is quite unlike any plasmid-encoded dihydrofolate reductase hitherto described. It has a low Mr (19,700) and has a higher specific activity than the constitutive Gram-negative plasmid dihydrofolate reductases. The type S1 enzyme is heat-stable and has a relatively low affinity for the substrate, dihydrofolate (Km 10.8 microM). It is moderately resistant to trimethoprim, and is competitively inhibited by this drug with an inhibitor-binding constant of 11.6 microM. This is the first identification and characterization of a plasmid-encoded trimethoprim-resistant dihydrofolate reductase derived from a Gram-positive species.

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Sequence of a Rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an APC-like permease

Canadian Journal of Microbiology ◽

10.1139/m94-066 ◽

1994 ◽

Vol 40 (5) ◽

pp. 403-407 ◽

Cited By ~ 7

Author(s):

René De Mot ◽

Istvan Nagy ◽

Geert Schoofs ◽

Jos Vanderleyden

Keyword(s):

Open Reading Frame ◽

Genomic Fragment ◽

Positive Bacterium ◽

Gram Positive ◽

Gene Encoding ◽

Reading Frame ◽

Transporter Family ◽

Rhodococcus Species ◽

Rhodococcus Sp

Sequence analysis of a 5173-bp genomic fragment from the nocardioform actinomycete Rhodococcus sp. strain NI86/21 revealed the presence of two genes, eutB and eutC, encoding the putative homologues of the large and small subunits of the ethanolamine ammonia-lyase, respectively, from Salmonella typhimurium. This is the first report of the characterization of these genes in a Gram-positive species. Immediately upstream of eutB, a gene encoding a putative permease of the APC (amino acids, polyamines, choline) transporter family was located. At present, no other Gram-positive members of this permease family are known. The translational coupling of these eut genes suggests an operon-like organization of the ethanolamine genes in Rhodococcus species. A truncated open reading frame downstream of eutC contained an N-terminal motif characteristic of membrane-anchored lipoproteins.Key words: nocardioform actinomycete, cobalamin, APC transporter, membrane-anchored lipoprotein, Gram-positive bacterium.

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Characterization of a Gram-Positive Bacterium from the Proventriculus of Budgerigars (Melopsittacus undulatus)

Avian Diseases ◽

10.2307/1591281 ◽

1990 ◽

Vol 34 (3) ◽

pp. 779 ◽

Cited By ~ 29

Author(s):

Charles M. Scanlan ◽

David L. Graham

Keyword(s):

Melopsittacus Undulatus ◽

Positive Bacterium ◽

Gram Positive

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Isolation and characterization of a novel hydrocarbon-degrading, Gram-positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. nov.

Journal of Applied Microbiology ◽

10.1046/j.1365-2672.2001.01241.x ◽

2001 ◽

Vol 90 (2) ◽

pp. 237-247 ◽

Cited By ~ 83

Author(s):

M.A. Engelhardt ◽

K. Daly ◽

R.P.J. Swannell ◽

I.M. Head

Keyword(s):

Positive Bacterium ◽

Gram Positive ◽

Beach Sediment ◽

Isolation And Characterization

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Identification and characterization of a biosynthetic gene cluster for tryptophan dimers in deep sea-derived Streptomyces sp. SCSIO 03032

Applied Microbiology and Biotechnology ◽

10.1007/s00253-017-8375-5 ◽

2017 ◽

Vol 101 (15) ◽

pp. 6123-6136 ◽

Cited By ~ 6

Author(s):

Liang Ma ◽

Wenjun Zhang ◽

Yiguang Zhu ◽

Guangtao Zhang ◽

Haibo Zhang ◽

...

Keyword(s):

Gene Cluster ◽

Deep Sea ◽

Biosynthetic Gene Cluster ◽

Biosynthetic Gene ◽

Streptomyces Sp ◽

Identification And Characterization

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Activation and Identification of a Griseusin Cluster in Streptomyces sp. CA-256286 by Employing Transcriptional Regulators and Multi-Omics Methods

Molecules ◽

10.3390/molecules26216580 ◽

2021 ◽

Vol 26 (21) ◽

pp. 6580

Author(s):

Charlotte Beck ◽

Tetiana Gren ◽

Francisco Javier Ortiz-López ◽

Tue Sparholt Jørgensen ◽

Daniel Carretero-Molina ◽

...

Keyword(s):

Biosynthetic Pathway ◽

Genome Mining ◽

Gene Clusters ◽

Transcriptional Regulators ◽

Biosynthetic Gene ◽

Biosynthetic Gene Clusters ◽

Heterologous Host ◽

Streptomyces Sp ◽

Identification And Characterization

Streptomyces are well-known producers of a range of different secondary metabolites, including antibiotics and other bioactive compounds. Recently, it has been demonstrated that “silent” biosynthetic gene clusters (BGCs) can be activated by heterologously expressing transcriptional regulators from other BGCs. Here, we have activated a silent BGC in Streptomyces sp. CA-256286 by overexpression of a set of SARP family transcriptional regulators. The structure of the produced compound was elucidated by NMR and found to be an N-acetyl cysteine adduct of the pyranonaphtoquinone polyketide 3′-O-α-d-forosaminyl-(+)-griseusin A. Employing a combination of multi-omics and metabolic engineering techniques, we identified the responsible BGC. These methods include genome mining, proteomics and transcriptomics analyses, in combination with CRISPR induced gene inactivations and expression of the BGC in a heterologous host strain. This work demonstrates an easy-to-implement workflow of how silent BGCs can be activated, followed by the identification and characterization of the produced compound, the responsible BGC, and hints of its biosynthetic pathway.

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