scholarly journals Structural characterization of two solute-binding proteins for N,N′-diacetylchitobiose/N,N′,N′′-triacetylchitotoriose of the gram-positive bacterium, Paenibacillus sp. str. FPU-7

2021 ◽  
pp. 100049
Author(s):  
Takafumi Itoh ◽  
Misaki Yaguchi ◽  
Akari Nakaichi ◽  
Moe Yoda ◽  
Takao Hibi ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Binding Proteins ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Paenibacillus Sp

Sequence of a Rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an APC-like permease

1994 ◽  
Vol 40 (5) ◽  
pp. 403-407 ◽  
Author(s):  
René De Mot ◽  
Istvan Nagy ◽  
Geert Schoofs ◽  
Jos Vanderleyden
Keyword(s):  
Open Reading Frame ◽  
Genomic Fragment ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Gene Encoding ◽  
Reading Frame ◽  
Transporter Family ◽  
Rhodococcus Species ◽  
Rhodococcus Sp

Sequence analysis of a 5173-bp genomic fragment from the nocardioform actinomycete Rhodococcus sp. strain NI86/21 revealed the presence of two genes, eutB and eutC, encoding the putative homologues of the large and small subunits of the ethanolamine ammonia-lyase, respectively, from Salmonella typhimurium. This is the first report of the characterization of these genes in a Gram-positive species. Immediately upstream of eutB, a gene encoding a putative permease of the APC (amino acids, polyamines, choline) transporter family was located. At present, no other Gram-positive members of this permease family are known. The translational coupling of these eut genes suggests an operon-like organization of the ethanolamine genes in Rhodococcus species. A truncated open reading frame downstream of eutC contained an N-terminal motif characteristic of membrane-anchored lipoproteins.Key words: nocardioform actinomycete, cobalamin, APC transporter, membrane-anchored lipoprotein, Gram-positive bacterium.

scholarly journals Biophysical and Structural Characterization of a Sequence-diverse Set of Solute-binding Proteins for Aromatic Compounds

2012 ◽  
Vol 287 (28) ◽  
pp. 23748-23756 ◽  
Author(s):  
Ruth Pietri ◽  
Sarah Zerbs ◽  
Danielle M. Corgliano ◽  
Marc Allaire ◽  
Frank R. Collart ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Aromatic Compounds ◽  
Binding Proteins
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