Enhancement of pipecolic acid production by the expression of multiple lysine cyclodeaminase in the Escherichia coli whole-cell system

2020 ◽  
Vol 140 ◽  
pp. 109643
Author(s):  
Yeong-Hoon Han ◽  
Tae-Rim Choi ◽  
Ye-Lim Park ◽  
Jun Young Park ◽  
Hun-Suk Song ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Cell System ◽  
Pipecolic Acid ◽  
Whole Cell

scholarly journals Enhancement of pipecolic acid production by the expression of multiple lysine cyclodeaminase in the Escherichia coli whole-cell system

Authorea ◽  
2020 ◽  
Author(s):  
Yeong Hoon Han ◽  
Tae Rim Choi ◽  
Ye Lim Park ◽  
Jun Young Park ◽  
Hun suk Song ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Cell System ◽  
Pipecolic Acid ◽  
Whole Cell

Whole-cell Immobilization of Engineered Escherichia coli JY001 with Barium-alginate for Itaconic Acid Production

2018 ◽  
Vol 23 (4) ◽  
pp. 442-447 ◽  
Author(s):  
Yu-Mi Moon ◽  
Ranjit Gurav ◽  
Junyoung Kim ◽  
Yun-Gi Hong ◽  
Shashi Kant Bhatia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Itaconic Acid ◽  
Acid Production ◽  
Cell Immobilization ◽  
Whole Cell ◽  
Engineered Escherichia Coli

scholarly journals A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Simone Brixius-Anderko ◽  
Lina Schiffer ◽  
Frank Hannemann ◽  
Bernd Janocha ◽  
Rita Bernhardt
Keyword(s):  
Escherichia Coli ◽  
Cell System ◽  
Biotechnological Production ◽  
Whole Cell

scholarly journals Ultrasound-assisted l-cysteine whole-cell bioconversion by recombinant Escherichia coli with tryptophan synthase

2021 ◽  
Vol 10 (1) ◽  
pp. 842-850
Author(s):  
Lisheng Xu ◽  
Furu Wu ◽  
Tingting Li ◽  
Xingtao Zhang ◽  
Qiong Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell System ◽  
Tryptophan Synthase ◽  
Cysteine Biosynthesis ◽  
Optimal Conditions ◽  
Whole Cell ◽  
G Cells ◽  
Biological Transformation ◽  
Ultrasound Assisted ◽  
Whole Cell Bioconversion

Abstract l-Cysteine is widely used in food, medicine, and cosmetics. In this study, a recombinant Escherichia coli whole-cell system with tryptophan synthase was used to complete the biological transformation of l-serine to l-cysteine, and bioconversion of l-cysteine was investigated by tryptophan synthase. The biotransformation of l-cysteine was optimized by response surface methodology. The optimal conditions obtained are 0.13 mol·L−1 l-serine, 75 min, 130 W ultrasound operation, where the V max of tryptophan synthase is 25.27 ± 0.16 (mmol·h−1·(g-cells)−1). The V max of tryptophan synthase for the biosynthesis without ultrasound is 12.91 ± 0.34 (mmol·h−1·(g-cells)−1). Kinetic analysis of the recombinant Escherichia coli whole-cell system with tryptophan synthase also showed that under the ultrasound treatment, the K m values of l-cysteine biosynthesis increase from 1.342 ± 0.11 mM for the shaking biotransformation to 2.555 ± 0.13 mM for ultrasound operation. The yield of l-cysteine reached 91% after 75 min of treatment after 130 W ultrasound, which is 1.9-fold higher than no ultrasound.

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