Trypanosoma cruzi: Isolation and characterization of aspartyl proteases

2009 ◽  
Vol 122 (2) ◽  
pp. 128-133 ◽  
Author(s):  
Rosa T. Pinho ◽  
Leila M. Beltramini ◽  
Carlos R. Alves ◽  
Salvatore G. De-Simone
Keyword(s):  
Trypanosoma Cruzi ◽  
Isolation And Characterization ◽  
Aspartyl Proteases

Isolation and characterization of the gene encoding histone H2A from Trypanosoma cruzi

1994 ◽  
Vol 64 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Concepción Puerta ◽  
Javier Martin ◽  
Carlos Alonso ◽  
Manuel C. López
Keyword(s):  
Trypanosoma Cruzi ◽  
Histone H2a ◽  
Gene Encoding ◽  
Isolation And Characterization

Trypanosoma cruzi: Isolation and characterization of membrane and flagellar fractions

1978 ◽  
Vol 46 (2) ◽  
pp. 225-234 ◽  
Author(s):  
Neize M. Pereira ◽  
Solange L. Timm ◽  
Sylvio Celso G. da Costa ◽  
Moacyr A. Rebello ◽  
Wanderley de Souza
Keyword(s):  
Trypanosoma Cruzi ◽  
Isolation And Characterization

Isolation and characterization of the Golgi complex of the protozoan Trypanosoma cruzi

Parasitology ◽  
2001 ◽  
Vol 123 (1) ◽  
pp. 33-43 ◽  
Author(s):  
J. A. MORGADO-DÍAZ ◽  
C. V. NAKAMURA ◽  
O. A. AGRELLOS ◽  
W. B. DIAS ◽  
J. O. PREVIATO ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Trypanosoma Cruzi ◽  
Golgi Complex ◽  
Mammalian Cells ◽  
Sucrose Density Gradient ◽  
Isolation And Characterization ◽  
Golgi Region ◽  
Electron Microscopy Analysis ◽  
Glcnac Transferase

In this study the Golgi complex of the epimastigote forms of Trypanosoma cruzi were isolated and characterized. Using well-controlled sonication to rupture the cells and centrifugation on a discontinuous sucrose density gradient, a highly enriched Golgi fraction was obtained. The Golgi fraction contained most of the β-galactosyltransferase (β-Gal transferase) and UDP-N-acetyl-glucosamine: polypeptide-α-N-acetyl-glucosaminyltransferase (O-α-GlcNAc transferase) activities with minimal contamination of other organelles, as observed by enzymatic assays and electron microscopy analysis. To characterize the Golgi from T. cruzi cells further, it was incubated with a monoclonal antibody against a 58 kDa protein involved in the association of the Golgi complex with microtubules in mammalian cells. Immunofluorescence microscopy showed that the 58 kDa protein is localized in the T. cruzi Golgi region, a result confirmed by high resolution scanning electron microscopy immunocytochemistry. Thus, our results show, for the first time, that the β-Gal transferase, the O-α-GlcNAc transferase and the 58 kDa protein are present in the Golgi complex of T. cruzi and are novel biochemical markers which can be used in the characterization of this organelle in T. cruzi.

scholarly journals Isolation and Characterization of Paraflagellar Proteins from Trypanosoma cruzi

1989 ◽  
Vol 264 (7) ◽  
pp. 4071-4075
Author(s):  
J L Saborio ◽  
J Manuel Hernandez ◽  
S Narayanswami ◽  
R Wrightsman ◽  
E Palmer ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Isolation And Characterization

Trypanosoma cruzi: Isolation and characterization of a protease

1978 ◽  
Vol 45 (1) ◽  
pp. 8-18 ◽  
Author(s):  
Vera Bongertz ◽  
Klaus Dieter Hungerer
Keyword(s):  
Trypanosoma Cruzi ◽  
Isolation And Characterization

Trypanosoma cruzi: Isolation and characterization of a proteinase

1981 ◽  
Vol 52 (2) ◽  
pp. 199-209 ◽  
Author(s):  
H.A. Rangel ◽  
P.M.F. Araújo ◽  
D. Repka ◽  
M.G. Costa
Keyword(s):  
Trypanosoma Cruzi ◽  
Isolation And Characterization
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