scholarly journals Biological Variation of Donor-Derived Cell-Free DNA in Lung Transplant Recipients

2021 ◽  
Vol 40 (4) ◽  
pp. S338-S339
Author(s):  
M.B. Keller ◽  
C. Mutebi ◽  
P. Shah ◽  
D. Levine ◽  
S. Aryal ◽  
...  
Keyword(s):  
Lung Transplant ◽  
Biological Variation ◽  
Transplant Recipients ◽  
Cell Free Dna ◽  
Free Dna ◽  
Lung Transplant Recipients

scholarly journals A Longitudinal Assessment of Donor Derived Cell Free DNA in Lung Transplant Recipients

2021 ◽  
Vol 40 (4) ◽  
pp. S320-S321
Author(s):  
J.B. Smith ◽  
J. Stumph ◽  
Y. Bryan ◽  
R. Peterson ◽  
M.P. Steele ◽  
...  
Keyword(s):  
Lung Transplant ◽  
Transplant Recipients ◽  
Longitudinal Assessment ◽  
Cell Free Dna ◽  
Free Dna ◽  
Lung Transplant Recipients

scholarly journals New Clue: Prediction from Cell-Free DNA

2020 ◽  
Vol 9 (7) ◽  
pp. 2307
Author(s):  
Yan Y. Sanders
Keyword(s):  
Lung Transplant ◽  
Disease Diagnosis ◽  
Transplant Recipients ◽  
Term Survival ◽  
Cell Free Dna ◽  
Long Term Outcome ◽  
Free Dna ◽  
Main Challenge ◽  
Lung Transplant Recipients

The main challenge for a positive long-term outcome in lung transplantation is the lack of early detection for chronic lung allograft dysfunction (CLAD). With advancements in technology, an increasing number of studies demonstrate that cell-free DNA (cfDNA) in body fluids could be used as a marker for disease diagnosis, prognosis or monitoring response to treatment. A previous report from this journal found the joint assessment of cfDNA and CXCL10 from brochoalveolar lavage (BAL) could determine the subphenotypes of CLAD and predict lung transplant survival. This is an exciting attempt in monitoring the progress for lung transplant recipients. More studies and better understanding of cfDNA are needed to develop an accessible and reliable biomarker to monitor the progress of CLAD to improve the long-term survival for lung transplant recipients.

scholarly journals Single-stranded DNA library preparation uncovers the origin and diversity of ultrashort cell-free DNA in plasma

2015 ◽  
Author(s):  
Philip Burnham ◽  
Min Seong Kim ◽  
Sean Agbor-Enoh ◽  
Helen Luikart ◽  
Hannah A Valantine ◽  
...  
Keyword(s):  
Lung Transplant ◽  
Preparation Method ◽  
Fragmentation Pattern ◽  
Library Preparation ◽  
Transplant Recipients ◽  
Cell Free Dna ◽  
Single Stranded Dna ◽  
Free Dna ◽  
Lung Transplant Recipients ◽  
Library Preparation Method

Circulating cell-free DNA (cfDNA) is emerging as a powerful monitoring tool in cancer, pregnancy and organ transplantation. Nucleosomal DNA, the predominant form of cfDNA in blood, can be readily adapted for sequencing via ligation of double-stranded DNA (dsDNA) adapters. dsDNA library preparation, however, is insensitive to ultrashort, degraded and single-stranded cfDNA. Drawing inspiration from recent technical advances in ancient genome analyses, we have applied a single-stranded DNA (ssDNA) library preparation method to sequencing of cfDNA in the plasma of lung transplant recipients (40 samples, six patients). We found that the ssDNA library preparation yields a greater portion of sub-100 bp DNA, as well as an increased relative abundance of human mitochondrial cfDNA (10.7x) and microbial cfDNA (71.3x). We report the fragmentation pattern of mitochondrial, nuclear genomic and microbial cfDNA over a broad fragment length range. We furthermore report the first observation of donor-specific mitochondrial cfDNA in the circulation of lung transplant recipients. We found that donor-specific mitochondrial cfDNA molecules are significantly shorter than those specific to the recipient. The higher yield of viral, microbial and fungal sequences that result from the single-stranded ligation approach reduces the cost and increase the sensitivity of cfDNA-based monitoring for infectious complications after transplantation. An ssDNA library preparation method provides a more informative window into understudied forms of cfDNA, including mitochondrial and microbial derived cfDNA and short fragment nuclear genomic cfDNA, while retaining information provided by standard dsDNA library preparation methods.

Detection of donor cell-free dna using digital PCR in lung transplant recipients with graft rejection and infection

2015 ◽  
Vol 76 ◽  
pp. 68 ◽  
Author(s):  
Jun Zou ◽  
Brian Duffy ◽  
Nancy Steward ◽  
Ramsey Hachem ◽  
Thalachallour Mohanakumar
Keyword(s):  
Graft Rejection ◽  
Lung Transplant ◽  
Donor Cell ◽  
Digital Pcr ◽  
Transplant Recipients ◽  
Cell Free Dna ◽  
Free Dna ◽  
Lung Transplant Recipients

scholarly journals Rapid detection of donor cell free DNA in lung transplant recipients with rejections using donor-recipient HLA mismatch

2017 ◽  
Vol 78 (4) ◽  
pp. 342-349 ◽  
Author(s):  
Jun Zou ◽  
Brian Duffy ◽  
Michael Slade ◽  
Andrew Lee Young ◽  
Nancy Steward ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lung Transplant ◽  
Donor Cell ◽  
Transplant Recipients ◽  
Cell Free Dna ◽  
Free Dna ◽  
Lung Transplant Recipients

scholarly journals Biological Variation of Donor-Derived Cell-Free DNA in Renal Transplant Recipients: Clinical Implications

2017 ◽  
Vol 2 (3) ◽  
pp. 309-321 ◽  
Author(s):  
Jonathan S. Bromberg ◽  
Daniel C. Brennan ◽  
Emilio Poggio ◽  
Suphamai Bunnapradist ◽  
Anthony Langone ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Biological Variation ◽  
Clinical Implications ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Cell Free Dna ◽  
Free Dna
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