Molecular mechanism of increasing extracellular polysaccharide production of Paenibacillus mucilaginosus K02 by adding mineral powders

2022 ◽  
Vol 167 ◽  
pp. 105340
Author(s):  
Xiaofang Li ◽  
Yangge Yu ◽  
Ying Li ◽  
Zuoying Yin ◽  
Xiuming Liu ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Extracellular Polysaccharide ◽  
Polysaccharide Production ◽  
Paenibacillus Mucilaginosus

scholarly journals Effect of medium pH and cultivation period on mycelial biomass, polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum from Montenegro

2006 ◽  
Vol 58 (3) ◽  
pp. 179-182 ◽  
Author(s):  
Jelena Vukojevic ◽  
Mirjana Stajic ◽  
Sonja Duletic-Lausevic ◽  
Jasmina Simonic
Keyword(s):  
Ganoderma Lucidum ◽  
Enzyme Production ◽  
Ligninolytic Enzymes ◽  
Extracellular Polysaccharide ◽  
Ligninolytic Enzyme ◽  
Extracellular Polysaccharides ◽  
Medium Ph ◽  
Polysaccharide Production ◽  
Intracellular Polysaccharide ◽  
Maximal Production

The effect of initial medium pH on biomass, extracellular and intracellular polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum was investigated at different pH values after 7 and 14 days of cultivation. Maximal production of biomass was recorded at pH 4.5 and 5.0; maximal production of extracellular polysaccharides at pH 7.0 and 3.0; and maximal production of intracellular polysaccharides at pH 7.0 and 5.5. Ligninolytic enzymes were not produced at any pH of the medium. Maximal biomass production was obtained on the 11th day of cultivation; maximal extracellular polysaccharide production on the 7th day; and maximal intracellular polysaccharide production on the 6th and 10th day of cultivation. .

Nodulation competitiveness of Tn5-induced mutants of Rhizobium fredii USDA208 that are altered in motility and extracellular polysaccharide production

1991 ◽  
Vol 37 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Robert E. Zdor ◽  
Steven G. Pueppke
Keyword(s):  
Parental Strain ◽  
Extracellular Polysaccharide ◽  
Triphenyltetrazolium Chloride ◽  
Polysaccharide Production ◽  
Production Type ◽  
Rhizobium Fredii ◽  
Induced Mutants ◽  
Nodulation Competitiveness

The role of motility and extracellular polysaccharide production in nodulation competitiveness of Rhizobium fredii was examined. Transposon Tn5 was used to mutagenize strain USDA208, and mutants with reduced motility on semisolid agar medium were isolated. One such mutant, 208M3, migrated to only one-seventh the distance of the parental strain. Solid medium amended with triphenyltetrazolium chloride was used to identify mutants altered in extracellular polysaccharide production. Type 1 colonies, typified by mutant 208T13, were heavily mucoid, while type 2 colonies, represented by mutant 208T3, were dry and nonmucoid. Compared with strain USDA208, these mutants produced 4- to 5-fold more extracellular polysaccharide and 20% as much extracellular polysaccharide, respectively. Marker exchange of 208T3 genomic DNA containing Tn5 into strain USDA208 resulted in a mutant, 208K1, that produced extracellular polysaccharide levels similar to mutant 208T3. Mutants 208M3, 208T3, and 208T13 contained single Tn5 insertions. All formed pink nodules on 'Peking' soybean that were structurally indistinguishable and contained proteins with similar profiles. Rates of nodulation were similar in the mutants and the parental strain. Mutants 208M3 and 208T13 were as competitive against an isolate of Bradyrhizobium japonicum serogroup 123 as was strain USDA208. In contrast, mutants 208T3 and 208K1 were competitively superior. Key words: nodulation competition, motility, extracellular polysaccharide, Rhizobium.

Effects of Fluoride, Lithium and Strontium on Extracellular Polysaccharide Production by Streptococcus mutans and Actinomyces viscosus

1981 ◽  
Vol 60 (C) ◽  
pp. 1601-1610 ◽  
Author(s):  
Patrick Treasure
Keyword(s):  
Trace Elements ◽  
Streptococcus Mutans ◽  
Extracellular Polysaccharide ◽  
Water Soluble ◽  
Extracellular Polysaccharides ◽  
Polysaccharide Production

Effects of trace elements on production of extracellular polysaccharides (EPS) by S. mutans and A. viscosus were examined in vitro. Fluoride enhanced EPS production. Lithium and strontium had little effect alone, but tended to reverse the effect of fluoride. The proportion of water-soluble EPS and the proportion of glucosyl-EPS were increased by fluoride.

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