Based on the data of land use in 1999, 2005, 2010 and 2020, selected the typical tourist area Yangshuo and research the land use changes on ecosystem service value and then estimated the value change. Throughout the study period, Yangshuo County has been always implement tourism development strategy, the unused land substantially reduced and transformed into woodland, water body and wetland, which makes the ecological environment has been improved. The total value of ecosystem services in Yangshuo County was 1067.4 million Yuan in 1999, 1165 million Yuan in 2005, 1478.3 million Yuan in 2010, from 1999 to 2020, the ecosystem service values increased 1002.9 million Yuan, it is mainly due to the increasing areas of woodland, water body and wetland. The combined ecosystem service value of woodland and cropland was about 90% of the total value. The contribution of each ecosystem function to the overall ESV was greatest for soil formation and retention, followed by water supply, both of them contributing about 34% of the total value. Yangshuo County implement eco-tourism, landscape of leisure tourism and agro-tourism as the development strategy, it provides a strong protection to protect the local environment, and promote the ecosystem service values on the rise.