Characterization of Three Novel Wheat−Thinopyrum intermedium Addition Lines with Novel Storage Protein Subunits and Resistance to Both Powdery Mildew and Stripe Rust

2016 ◽  
Vol 43 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Yuhai Wang ◽  
Honggang Wang
Keyword(s):  
Powdery Mildew ◽  
Stripe Rust ◽  
Storage Protein ◽  
Addition Lines ◽  
Thinopyrum Intermedium ◽  
Protein Subunits

Disomic Thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistances

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 385-394 ◽  
Author(s):  
P. J. Larkin ◽  
P. M. Banks ◽  
E. S. Lagudah ◽  
R. Appels ◽  
Chen Xiao ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Stem Rust ◽  
Rust Resistance ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Addition Lines ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Group 2 ◽  
Yellow Dwarf Virus

Zhong 5 is a partial amphiploid (2n = 56) between Triticum aestivum (2n = 42) and Thinopyrum intermedium (2n = 42) carrying all the chromosomes of wheat and seven pairs of chromosomes from Th. intermedium. Following further backcrossing to wheat, six independent stable 2n = 44 lines were obtained representing 4 disomic chromosome addition lines. One chromosome confers barley yellow dwarf virus (BYDV) resistance, whereas two other chromosomes carry leaf and stem rust resistance; one of the latter also confers stripe rust resistance. Using RFLP and isozyme markers we have shown that the extra chromosome in the Zhong 5-derived BYDV resistant disomic addition lines (Z1, Z2, or Z6) belongs to the homoeologous group 2. It therefore carries a different locus to the BYDV resistant group 7 addition, L1, described previously. The leaf, stem, and stripe rust resistant line (Z4) carries an added group 7 chromosome. The line Z3 has neither BYDV nor rust resistance, is not a group 2 or group 7 addition, and is probably a group 1 addition. The line Z5 is leaf and stem rust resistant, is not stripe rust resistant, and its homoeology remains unknown.Key words: Agropyron, intermediate wheatgrass, leaf rust, stem rust, stripe rust, luteovirus.

Assessment of resistance to cereal cyst nematode, stripe rust and powdery mildew in the wheat-Thinopyrum intermedium derivatives and their chromosome composition

Plant Disease ◽  
2021 ◽  
Author(s):  
Lei Cui ◽  
Yongkang Ren ◽  
Yinguang Bao ◽  
Hai Nan ◽  
Zhaohui Tang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Powdery Mildew ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Thinopyrum Intermedium ◽  
Resistant Lines ◽  
Multiple Pathogens

Wide hybridization between wheat and wild relatives such as Thinopyrum intermedium is important for broadening genetic diversity and improving disease resistance in wheat. We developed 30 wheat-Th. intermedium derivatives. Here, we report assessments of their resistance to different pathogens including cereal cyst nematode (CCN, Heterodera spp.), Puccinia striiformis causing stripe rust, and Blumeria graminis f. sp. tritici inciting powdery mildew. Under natural field infection, all the wheat-Th. intermedium lines were resistant to at least one of the pathogens, and four lines were resistant to multiple pathogens. Twenty-nine out of 30 tested lines exhibited resistance to H. avenae, a dominant CCN species in wheat fields. Twenty-four lines were resistant to H. filipjevi, an emerging threat to wheat production. Tests of phenotypic responses in the naturally infected field nurseries identified six stripe rust resistant lines and 13 powdery mildew resistant lines. Mitotic observation demonstrated that these newly developed wheat-Th. intermedium derivatives included not only octoploid but also chromosome addition, substitution, and translocation lines. Chromosome compositions of the four lines resistant to multiple pathogens were analyzed by genomic in situ hybridization and fluorescence in situ hybridization. The octoploid lines Zhong 10-68 and Zhong 10-117 carried both intact Th. intermedium chromosomes and translocated chromosomes. Line Zhong 10-149 had 42 wheat chromosomes and two wheat ditelosomes plus a pair of T3BS·J translocated chromosomes. Line Zhong 10-160 carried 41 wheat chromosomes plus one pair of the J genome chromosomes of Th. intermedium. The multiple disease resistant wheat-Th. intermedium derivatives, especially lines with chromosome counts close to common wheat, provide valuable materials for wheat resistance breeding programs.

Development and characterization of a new 1BL.1RS translocation line with resistance to stripe rust and powdery mildew of wheat

Euphytica ◽  
2009 ◽  
Vol 169 (2) ◽  
pp. 207-213 ◽  
Author(s):  
Tian-Heng Ren ◽  
Zu-Jun Yang ◽  
Ben-Ju Yan ◽  
Huai-Qiong Zhang ◽  
Shu-Lan Fu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Stripe Rust ◽  
Translocation Line

Identification of wheat-Thinopyrum intermedium alien disomic addition lines conferring resistance to stripe rust

2009 ◽  
Vol 89 (3) ◽  
pp. 569-574 ◽  
Author(s):  
F. Lin ◽  
Q. Sun ◽  
S. Xu ◽  
X. Chen ◽  
L. Zhang ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Dominant Gene ◽  
Stripe Rust Resistance ◽  
Root Tip ◽  
Addition Lines ◽  
Thinopyrum Intermedium ◽  
Disomic Addition Lines

Thinopyrum intermedium carries many useful traits for wheat genetic improvement. To identify genes conferring resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in Zhong4, one of the Zhong series of partial amphiploids from the hybrids of common wheat × Th. intermedium, a cross was made between Zhong4 and a Chinese Spring ph1b mutant (CS-ph1b). The parents, F1, F2 plants and F5 lines of the cross were tested with Chinese predominant race CYR31 of P. striiformis f. sp. tritici in the seedling stage under controlled greenhouse conditions. Of 201 F2 seedlings tested with race CYR31, 149 were resistant and 52 were susceptible. The segregation fit a 3 resistant:1 susceptible ratio, indicating that a single dominant gene conferred resistance to the race CYR31. This result was further confirmed by the segregation of F5 lines into 1:1 ratio (P = 0.77). Using the Schiff dyeing method, the chromosome number in the root-tip cells of 10 F5 resistant lines (total 42 plants) was determined. Two alien addition lines (5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n = 42 + II) were identified. A total of 105 pollen mother cells were examined at metaphase 1 (PMCs MI) for meiotic chromosome pairing. The lines 5-1-2-1-1-5 and 5-1-2-1-1-6 showed regular meiosis, exhibiting 22 ring or rod bivalents (2n = 22 II). The GISH results indicated that lines 5-1-2-1-1-5 and 5-1-2-1-1-6 were wheat-Th. intermedium alien disomic addition lines with 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes. These two lines were all resistant to stripe rust, suggesting that they can be used as donors of stripe rust resistance genes and have great potential to play important roles in wheat stripe rust resistance breeding programs in China and the world.Key words: Wheat-Thinopyrum intermedium, stripe rust, GISH, cytological identification

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