Quantification and genotyping of hepatitis B virus in a single reaction by real-time PCR and melting curve analysis

2004 ◽  
Vol 41 (4) ◽  
pp. 659-666 ◽  
Author(s):  
Shiou-Hwei Yeh ◽  
Ching-Yi Tsai ◽  
Jia-Horng Kao ◽  
Chun-Jen Liu ◽  
Ti-Jung Kuo ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
B Virus ◽  
Single Reaction

scholarly journals Applicability of real-time PCR combined with melting curve analysis for the study of hepatitis B virus genome mutations

Hepatology ◽  
2003 ◽  
Vol 37 (2) ◽  
pp. 478-479 ◽  
Author(s):  
Francisco Rodriguez-Frias ◽  
Rosendo Jardi ◽  
Maria Buti ◽  
Rafael Esteban
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Virus Genome ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
B Virus

Simultaneous quantitation and genotyping of hepatitis B virus by real-time PCR and melting curve analysis

2004 ◽  
Vol 120 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Sunchai Payungporn ◽  
Pisit Tangkijvanich ◽  
Pojchanad Jantaradsamee ◽  
Apiradee Theamboonlers ◽  
Yong Poovorawan
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
B Virus

Determination of hepatitis B virus (HBV) genotype D with real-time PCR (melting curve analysis) method

2003 ◽  
Vol 38 ◽  
pp. 111
Author(s):  
C. Eroglu ◽  
H. Leblebicioglu ◽  
M. Gunaydin ◽  
D. Turan ◽  
M. Sunbul ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Analysis Method ◽  
Hbv Genotype ◽  
B Virus

scholarly journals MELTING CURVE ANALYSIS FOR THE SCREENING OF HEPATITIS B VIRUS GENOTYPES A, D AND F IN PATIENTS FROM A GENERAL HOSPITAL IN SOUTHERN BRAZIL

2013 ◽  
Vol 50 (3) ◽  
pp. 219-225 ◽  
Author(s):  
Carlos Eduardo BECKER ◽  
Nelson Alexandre KRETZMANN ◽  
Angelo Alves de MATTOS ◽  
Ana Beatriz Gorini da VEIGA
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Nested Pcr ◽  
Melting Curve ◽  
Accurate Method ◽  
Melting Curve Analysis ◽  
Southern Brazil ◽  
Pcr Multiplex ◽  
B Virus

Context Hepatitis B virus (HBV) can cause fulminant hepatitis, cirrhosis and hepatocellular carcinoma, and is one of the most common causes of acute and chronic liver failure. The genetic variants of HBV can be decisive for the evolution of these diseases as well as for the election of therapy. Objectives The aim of this study was to evaluate and standardize an in house methodology based on the analysis of the melting curve polymerase chain reaction (PCR) of real-time (qPCR) to screen for genotypes A, D and F of HBV in patients from a hospital in Rio Grande do Sul, Brazil. Methods We evaluated 104 patients presumably with HBV chronic infection. Viral DNA was extracted from plasma and viral genotypes and different mutations were determined using PCR-based protocols. Results A PCR-based methodology was standardized for the analysis of genotypes A, D and F of HBV. The technique was based in a nested PCR with the final step consisting of a multiplex real-time PCR, using the melting curve as a tool for the differentiation of fragments. A higher frequency of genotype D (44.4%), followed by genotype A (22.2%) and genotype F (3.7%) was observed. Conclusion The standardized assay, a nested PCR-multiplex qPCR using specific primers, provides a rapid and accurate method for the differentiation of HBV genotypes that are more frequent in Southern Brazil – A, D and F. This method can be applied in the clinical practice.

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