Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system

2016 ◽  
Vol 133 ◽  
pp. 87-94 ◽  
Author(s):  
Sehroon Khan ◽  
Sadia Nadir ◽  
Guo Lihua ◽  
Jianchu Xu ◽  
Keith A. Holmes ◽  
...  
2016 ◽  
Vol 100 (20) ◽  
pp. 8889-8900 ◽  
Author(s):  
Sihyeon Kim ◽  
Se Jin Lee ◽  
Yu-Shin Nai ◽  
Jeong Seon Yu ◽  
Mi Rong Lee ◽  
...  

2021 ◽  
Author(s):  
Najie Shi ◽  
Fei Hu ◽  
Ping Wang ◽  
Yuxiang Zhang ◽  
Qiuyan Zhu ◽  
...  

Abstract The entomopathogenic fungus Beauveria bassiana is used worldwide for its biological control. Seven dsRNA segments were detected from a single Beauveria bassiana strain RCEF 1446. High-throughput sequencing indicated the presence of three mycoviruses in the infected sample. Two known mycoviruses were identified as Beauveria bassiana Victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as Beauveria bassiana bipartite mycovirus 1 (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The first dsRNA is 2,026 bp in length and encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the second segment encoding a hypothetical protein (35.55 kDa) of unknown function, was 1,810 bp in length. Moreover, the RdRp protein sequences showed the highest identity of 62.89% to Corynespora cassiicola bipartite mycovirus 1. Phylogenetic analysis of the RdRp reveals that the virus represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.


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