LNA probe-based real-time RT-PCR for the detection of infectious bronchitis virus from the oviduct of unvaccinated and vaccinated laying hens

2009 ◽  
Vol 155 (1) ◽  
pp. 67-71 ◽  
Author(s):  
K.K. Chousalkar ◽  
B.F. Cheetham ◽  
J.R. Roberts
Keyword(s):  
Real Time ◽  
Infectious Bronchitis Virus ◽  
Laying Hens ◽  
Rt Pcr ◽  
Infectious Bronchitis ◽  
Lna Probe

scholarly journals Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens

2006 ◽  
Vol 138 (1-2) ◽  
pp. 60-65 ◽  
Author(s):  
Scott A. Callison ◽  
Deborah A. Hilt ◽  
Tye O. Boynton ◽  
Brenda F. Sample ◽  
Robert Robison ◽  
...  
Keyword(s):  
Real Time ◽  
Infectious Bronchitis Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Infectious Bronchitis

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran

2004 ◽  
Vol 52 (2) ◽  
pp. 163-166 ◽  
Author(s):  
M. R. Seyfi Abad Shapouri ◽  
M. Mayahi ◽  
K. Assasi ◽  
S. Charkhkar
Keyword(s):  
Virus Type ◽  
Infectious Bronchitis Virus ◽  
Nested Pcr ◽  
Vaccination Programme ◽  
Rna Extraction ◽  
Viral Rna ◽  
Rt Pcr ◽  
Infectious Bronchitis ◽  
High Prevalence

To evaluate the prevalence of infectious bronchitis virus (IBV) type 4/91 in Iran, tracheal swabs from 77 broiler flocks in 16 provinces were collected at the slaughterhouse. Swabs were subjected to RNA extraction and tested by RT-PCR, followed by a type-specific nested PCR. The viral RNA was detected in 33 samples (42.8%) from different provinces. The results indicate a relatively high prevalence of IBV type 4/91 in Iran and necessitate revising the vaccination programme against this disease.

Differentiation of infectious bronchitis virus vaccine strains Ma5 and 4/91 by TaqMan real-time PCR

2017 ◽  
Vol 20 (3) ◽  
pp. 599-601 ◽  
Author(s):  
T. Stenzel ◽  
D. Dziewulska ◽  
M. Śmiałek ◽  
B. Tykałowski ◽  
J. Kowalczyk ◽  
...  
Keyword(s):  
Real Time ◽  
Virus Vaccine ◽  
Infectious Bronchitis Virus ◽  
Real Time Pcr ◽  
Cross Reactivity ◽  
Specific Primers ◽  
Assay Sensitivity ◽  
Infectious Bronchitis ◽  
Molecular Assays ◽  
Rapid Molecular Assays

Abstract The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 × 103 copies of the Ma5 strain, and 3.852 x 103 copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV.

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