Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

Appearance of Haemagglutinability of Infectious Bronchitis Virus Following In Vitro and In Vivo Tracheal Passages

2010 ◽  
Vol 32 (1-10) ◽  
pp. 736-743
Author(s):  
Shunsuke Yachida ◽  
Kazunari Sawaguchi ◽  
Shigemi Aoyama ◽  
Yoshikazu Iritani ◽  
Yoshiyuki Hayashi
Keyword(s):  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis

scholarly journals Avian coronavirus infectious bronchitis virus susceptibility to botanical oleoresins and essential oils in vitro and in vivo

2010 ◽  
Vol 149 (1) ◽  
pp. 86-94 ◽  
Author(s):  
M.W. Jackwood ◽  
R. Rosenbloom ◽  
M. Petteruti ◽  
D.A. Hilt ◽  
A.W. McCall ◽  
...  
Keyword(s):  
Essential Oils ◽  
Infectious Bronchitis Virus ◽  
Virus Susceptibility ◽  
Infectious Bronchitis

scholarly journals Genetic grouping of avian infectious bronchitis virus isolated in Brazil based on RT-PCR/RFLP analysis of the S1 gene

2008 ◽  
Vol 28 (3) ◽  
pp. 190-194 ◽  
Author(s):  
Maria de Fátima S. Montassier ◽  
Liana Brentano ◽  
Hélio J. Montassier ◽  
Leonardo J. Richtzenhain
Keyword(s):  
Infectious Bronchitis Virus ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Avian Infectious Bronchitis Virus ◽  
Rt Pcr ◽  
Infectious Bronchitis ◽  
Glycoprotein Gene ◽  
Pcr Rflp ◽  
Commercial Poultry ◽  
Chicken Eggs

Twelve Brazilian isolates and one reference vaccine strain of avian infectious bronchitis virus (IBV) were propagated in embryonating chicken eggs. The entire S1 glycoprotein gene of these viruses was analysed by reverse-transcriptase-polymerase chain reaction and restriction fragment length polymorphism (RT-PCR-RFLP), using the restriction enzymes HaeIII, XcmI and BstyI. The RFLP patterns led to the classification of these isolates into five distinct genotypes: A, B, C, D and Massachusetts. Five of twelve isolates were grouped in Massachusetts genotype and the remaining seven viruses were classified into four distinct genotypes: A (2), B (2), C (2) or D (1). Such genotyping classification agreed with previous immunological analysis for most of these viruses, highlighting the occurrence of a relevant variability among the IBV strains that are circulating in Brazilian commercial poultry flocks.

scholarly journals Quantitative RT-PCR Assay for VEGF Mrna in Human Tumors of the Kidney

1999 ◽  
Vol 14 (4) ◽  
pp. 247-250 ◽  
Author(s):  
C. Tricarico ◽  
B. Salvadori ◽  
D. Villari ◽  
G. Nicita ◽  
A. Della Melina ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Clinical Laboratory ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Vegf Mrna ◽  
Expression Ratio ◽  
Vegf Mrna Expression

Angiogenesis is the formation of new capillaries from pre-existing vessels, and recent evidence has demonstrated that tumor growth is controlled mainly by angiogenesis. Vascular endothelial growth factor (VEGF) is an endothelium-specific growth factor which is strongly angiogenic in vitro and in vivo. We have developed a quantitative RT-PCR assay for the measurement of VEGF mRNA expression using a real-time procedure based on the use of fluorogenic probes and the ABI PRISM 7700 Sequence Detector System. The assay performance of this method in terms of practicability and reliability is reported with results that seem promising for its widespread use in the clinical laboratory. The method has been applied to the measurement of mRNA of VEGF in human renal cell carcinomas (RCC). Preliminary results show a significantly higher VEGF mRNA expression (ratio values between 181 and 2222) in tumor specimens compared to non-adjacent, non-tumoral tissue of the same subjects.

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