Development of a universal RT-PCR for amplifying and sequencing the leader and capsid-coding region of foot-and-mouth disease virus

2013 ◽  
Vol 189 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Lizhe Xu ◽  
William Hurtle ◽  
Jessica M. Rowland ◽  
Karissa A. Casteran ◽  
Stacey M. Bucko ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Coding Region ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Factors Required for the Uridylylation of the Foot-and-Mouth Disease Virus 3B1, 3B2, and 3B3 Peptides by the RNA-Dependent RNA Polymerase (3Dpol) In Vitro

2005 ◽  
Vol 79 (12) ◽  
pp. 7698-7706 ◽  
Author(s):  
Arabinda Nayak ◽  
Ian G. Goodfellow ◽  
Graham J. Belsham
Keyword(s):  
Rna Polymerase ◽  
Disease Virus ◽  
Rna Synthesis ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Coding Region ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Positive Strand Rna

ABSTRACT The 5′ terminus of picornavirus genomic RNA is covalently linked to the virus-encoded peptide 3B (VPg). Foot-and-mouth disease virus (FMDV) is unique in encoding and using 3 distinct forms of this peptide. These peptides each act as primers for RNA synthesis by the virus-encoded RNA polymerase 3Dpol. To act as the primer for positive-strand RNA synthesis, the 3B peptides have to be uridylylated to form VPgpU(pU). For certain picornaviruses, it has been shown that this reaction is achieved by the 3Dpol in the presence of the 3CD precursor plus an internal RNA sequence termed a cis-acting replication element (cre). The FMDV cre has been identified previously to be within the 5′ untranslated region, whereas all other picornavirus cre structures are within the viral coding region. The requirements for the in vitro uridylylation of each of the FMDV 3B peptides has now been determined, and the role of the FMDV cre (also known as the 3B-uridylylation site, or bus) in this reaction has been analyzed. The poly(A) tail does not act as a significant template for FMDV 3B uridylylation.

scholarly journals Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

2006 ◽  
Vol 37 (1) ◽  
pp. 121-132 ◽  
Author(s):  
Scott M. Reid ◽  
Satya Parida ◽  
Donald P. King ◽  
Geoffrey H. Hutchings ◽  
Andrew E. Shaw ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus

2017 ◽  
Vol 246 ◽  
pp. 90-94 ◽  
Author(s):  
Frank Vandenbussche ◽  
David J. Lefebvre ◽  
Ilse De Leeuw ◽  
Steven Van Borm ◽  
Kris De Clercq
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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