Strip-dried blood sampling: applicability for bovine leukemia virus detection with ELISA and real-time PCR

2019 ◽  
Vol 263 ◽  
pp. 101-104 ◽  
Author(s):  
Nikolay Yu. Saushkin ◽  
Jeanne V. Samsonova ◽  
Alexander P. Osipov ◽  
Sergey E. Kondakov
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Detection ◽  
Blood Sampling

scholarly journals Development of a real time PCR assay using SYBR Green chemistry for bovine leukemia virus detection

Retrovirology ◽  
2011 ◽  
Vol 8 (S1) ◽  
Author(s):  
Gonzalo Rama ◽  
Gonzalo Moratorio ◽  
Gonzalo Greif ◽  
Gonzalo Obal ◽  
Sergio Bianchi ◽  
...  
Keyword(s):  
Green Chemistry ◽  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Detection ◽  
Sybr Green ◽  
Pcr Assay

A novel real time PCR assay for bovine leukemia virus detection using mixed probes and degenerate primers targeting novel BLV strains

2021 ◽  
pp. 114264
Author(s):  
Liushiqi Borjigin ◽  
Shuji Yoneyama ◽  
Susumu Saito ◽  
Meripet Polat ◽  
Michihito Inokuma ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Detection ◽  
Pcr Assay ◽  
Degenerate Primers

scholarly journals Interlaboratory Comparison of Six Real-Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA

2018 ◽  
Vol 56 (7) ◽  
pp. e00304-18 ◽  
Author(s):  
J. P. Jaworski ◽  
A. Pluta ◽  
M. Rola-Łuszczak ◽  
S. L. McGowan ◽  
C. Finnegan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Dynamic Range ◽  
Leukemia Virus ◽  
Large Variability ◽  
Proviral Dna ◽  
International Panel ◽  
Pcr Assays ◽  
Dna Copy Numbers

ABSTRACTQuantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (OIE) reference laboratories and three collaborating laboratories to measure the interlaboratory variability of six already developed and available BLV qPCR assays. For that purpose, an international panel of 58 DNA samples reflecting the dynamic range of the majority of the assays was distributed to six testing centers. Based on qualitative results, the overall agreement among all six laboratories was moderate. However, significant variability in the measurement of the BLV proviral DNA copy number was observed among different laboratories. Quantitative PCR assays, even when performed by experienced staff, can yield large variability in BLV proviral DNA copy numbers without harmonization. Further standardization of different factors (i.e., utilization of unified protocols and unique calibrators) should increase interlaboratory agreement.

Evaluation of three different genomic regions for detection of bovine leukemia virus by real-time PCR

2021 ◽  
Author(s):  
Antônio Augusto Fonseca Júnior ◽  
Luciana Rabello Ferreira ◽  
Mateus Laguardia- Nascimento ◽  
Anselmo Vasconcelos Rivetti Júnior ◽  
Bruna Lopes Bueno ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Genomic Regions

Development of real time PCR for the detection of Bovine leukemia virus proviral DNA

2020 ◽  
Vol 23 (2) ◽  
pp. 20-27
Author(s):  
I.E. Gafarova ◽  
◽  
E.S. Lisitsina ◽  
Yu.A. Savochkina ◽  
N.V. Kirillova ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Proviral Dna

Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR

2013 ◽  
Vol 95 (3) ◽  
pp. 991-995 ◽  
Author(s):  
A. Forletti ◽  
M.A. Juliarena ◽  
C. Ceriani ◽  
A.F. Amadio ◽  
E. Esteban ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus

scholarly journals Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

2008 ◽  
Vol 123 (1-2) ◽  
pp. 81-89 ◽  
Author(s):  
Andrea N. Torres ◽  
Kevin P. O’Halloran ◽  
Laurie J. Larson ◽  
Ronald D. Schultz ◽  
Edward A. Hoover
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Virus Rna

scholarly journals Canine distemper virus detection in asymptomatic and non vaccinated dogs

2010 ◽  
Vol 30 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Helen L. Del Puerto ◽  
Anilton C. Vasconcelos ◽  
Luciana Moro ◽  
Fabiana Alves ◽  
Gissandra F. Braz ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Canine Distemper Virus ◽  
Early Stage ◽  
Virus Detection ◽  
Vero Cells ◽  
Canine Distemper ◽  
Coding Region ◽  
Protein Coding ◽  
Dissociation Curves

A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs. Samples from eleven domestic dogs with no signs of canine distemper and not vaccinated at the month of collection were used. Canine distemper virus vaccine samples in VERO cells were used as positive controls. RNA was isolated with Trizol®, and treated with a TURBO DNA-free kit. Primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). Canine b-actin (93 bp) was utilized as the endogenous control for normalization. Quantitative results of real time PCR generated by ABI Prism 7000 SDS Software showed that 54.5% of dogs with asymptomatic canine distemper were positive for canine distemper virus. Dissociation curves confirmed the specificity of the real time PCR fragments. This technique could detect even a few copies of viral RNA and identificate subclinically infected dogs providing accurate diagnosis of this disease at an early stage.

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