scholarly journals Development of a real time PCR assay using SYBR Green chemistry for bovine leukemia virus detection

Retrovirology ◽  
2011 ◽  
Vol 8 (S1) ◽  
Author(s):  
Gonzalo Rama ◽  
Gonzalo Moratorio ◽  
Gonzalo Greif ◽  
Gonzalo Obal ◽  
Sergio Bianchi ◽  
...  
Keyword(s):  
Green Chemistry ◽  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Detection ◽  
Sybr Green ◽  
Pcr Assay

A novel real time PCR assay for bovine leukemia virus detection using mixed probes and degenerate primers targeting novel BLV strains

2021 ◽  
pp. 114264
Author(s):  
Liushiqi Borjigin ◽  
Shuji Yoneyama ◽  
Susumu Saito ◽  
Meripet Polat ◽  
Michihito Inokuma ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Detection ◽  
Pcr Assay ◽  
Degenerate Primers

Strip-dried blood sampling: applicability for bovine leukemia virus detection with ELISA and real-time PCR

2019 ◽  
Vol 263 ◽  
pp. 101-104 ◽  
Author(s):  
Nikolay Yu. Saushkin ◽  
Jeanne V. Samsonova ◽  
Alexander P. Osipov ◽  
Sergey E. Kondakov
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Detection ◽  
Blood Sampling

Development of a real-time PCR assay using SYBR Green chemistry for monitoring Marek's disease virus genome load in feather tips

2006 ◽  
Vol 133 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Mohamed Faizal Abdul-Careem ◽  
Bruce D. Hunter ◽  
Éva Nagy ◽  
Leah R. Read ◽  
Babak Sanei ◽  
...  
Keyword(s):  
Green Chemistry ◽  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Virus Genome ◽  
Marek's Disease Virus ◽  
Sybr Green ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Pcr Assay

Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay

2007 ◽  
Vol 21 (5-6) ◽  
pp. 368-378 ◽  
Author(s):  
Anna Casabianca ◽  
Caterina Gori ◽  
Chiara Orlandi ◽  
Federica Forbici ◽  
Carlo Federico Perno ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Sybr Green ◽  
Sybr Green I ◽  
Quantitative Detection ◽  
Pcr Assay ◽  
Hiv Dna

scholarly journals Interlaboratory Comparison of Six Real-Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA

2018 ◽  
Vol 56 (7) ◽  
pp. e00304-18 ◽  
Author(s):  
J. P. Jaworski ◽  
A. Pluta ◽  
M. Rola-Łuszczak ◽  
S. L. McGowan ◽  
C. Finnegan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Leukemia Virus ◽  
Dynamic Range ◽  
Leukemia Virus ◽  
Large Variability ◽  
Proviral Dna ◽  
International Panel ◽  
Pcr Assays ◽  
Dna Copy Numbers

ABSTRACTQuantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (OIE) reference laboratories and three collaborating laboratories to measure the interlaboratory variability of six already developed and available BLV qPCR assays. For that purpose, an international panel of 58 DNA samples reflecting the dynamic range of the majority of the assays was distributed to six testing centers. Based on qualitative results, the overall agreement among all six laboratories was moderate. However, significant variability in the measurement of the BLV proviral DNA copy number was observed among different laboratories. Quantitative PCR assays, even when performed by experienced staff, can yield large variability in BLV proviral DNA copy numbers without harmonization. Further standardization of different factors (i.e., utilization of unified protocols and unique calibrators) should increase interlaboratory agreement.

scholarly journals A SYBR green I real-time polymerase chain reaction (PCR) assay for detection and quantification of Trichomonas gallinae

2020 ◽  
Vol 119 (11) ◽  
pp. 3909-3913
Author(s):  
Zaida Rentería-Solís ◽  
Tran Nguyen-Ho-Bao ◽  
Shahinaz Taha ◽  
Arwid Daugschies
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Small Subunit ◽  
Sybr Green ◽  
Pcr Assay ◽  
Standard Curve ◽  
Trichomonas Gallinae ◽  
Host Interaction

Abstract Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitro T. gallinae studies.

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