Immobilized recombinant Aspergillus oryzae expressing heterologous lipase: An efficient whole-cell biocatalyst for enantioselective transesterification in non-aqueous medium

2007 ◽  
Vol 48 (1-2) ◽  
pp. 33-37 ◽  
Author(s):  
Sriappareddy Tamalampudi ◽  
Shinji Hama ◽  
Takanori Tanino ◽  
Mahabubur Rahman Talukder ◽  
Akihiko Kondo ◽  
...  
Keyword(s):  
Aqueous Medium ◽  
Aspergillus Oryzae ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from Candida antarctica

2007 ◽  
Vol 75 (2) ◽  
pp. 387-395 ◽  
Author(s):  
Sriappareddy Tamalampudi ◽  
MD Mahabubur Rahman Talukder ◽  
Shinji Hama ◽  
Takanori Tanino ◽  
Yuya Suzuki ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Candida Antarctica ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Encoding Gene

Highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in Aspergillus oryzae

2011 ◽  
Vol 90 (3) ◽  
pp. 1171-1177 ◽  
Author(s):  
Tomohiro Takaya ◽  
Risa Koda ◽  
Daisuke Adachi ◽  
Kazunori Nakashima ◽  
Junpei Wada ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Biodiesel Production ◽  
Whole Cell ◽  
Highly Efficient ◽  
Whole Cell Biocatalyst ◽  
Lipase Expression

Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production

2011 ◽  
Vol 102 (12) ◽  
pp. 6723-6729 ◽  
Author(s):  
Daisuke Adachi ◽  
Shinji Hama ◽  
Takao Numata ◽  
Kazunori Nakashima ◽  
Chiaki Ogino ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Biodiesel Production ◽  
Partial Glyceride ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipases

2009 ◽  
Vol 108 ◽  
pp. S51-S52
Author(s):  
Daisuke Adachi ◽  
Kazunori Nakashima ◽  
Tsutomu Tanaka ◽  
Chiaki Ogino ◽  
Akihiko Kondo ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Biodiesel Production ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst
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