Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production

Adenosine triphosphate (ATP), as a universal energy currency, takes a central role in many biochemical reactions with potential for the synthesis of numerous high-value products. However, the high cost of ATP limits industrial ATP-dependent enzyme-catalyzed reactions. Here, we investigated the effect of cell-surface display of phosphotransferase on ATP regeneration in recombinant Escherichia coli. By N-terminal fusion of the super-folder green fluorescent protein (sfGFP), we successfully displayed the phosphotransferase of Pseudomonas brassicacearum (PAP-Pb) on the surface of E. coli cells. The catalytic activity of sfGFP-PAP-Pb intact cells was 2.12 and 1.47 times higher than that of PAP-Pb intact cells, when the substrate was AMP and ADP, respectively. The conversion of ATP from AMP or ADP were up to 97.5% and 80.1% respectively when catalyzed by the surface-displayed enzyme at 37 °C for only 20 min. The whole-cell catalyst was very stable, and the enzyme activity of the whole cell was maintained above 40% after 40 rounds of recovery. Under this condition, 49.01 mg/mL (96.66 mM) ATP was accumulated for multi-rounds reaction. This ATP regeneration system has the characteristics of low cost, long lifetime, flexible compatibility, and great robustness.

Increasing teacher professionalism through the regeneration system

The professional attitude of a teacher is no longer reflected at this time. Where they only carry out the task of transferring knowledge without providing direction and guidance that helps the development process of each student. The purpose of this research is to build and provide efforts to develop and improve the professional attitude of a teacher by carrying out a regeneration system with a recruitment process for every new cadre who wants to apply as a teacher. The method used in this research is a qualitative method with a literature study type approach. Where the researchers compared the results of several previous findings. From the results of this study it can be concluded that the principal has a very important role in using this regeneration system. Where the principal must always motivate the teachers. With the provision of motivation and enthusiasm, teachers will feel that they are getting an award in work performance.

The effect of 2,4-dichlorophenoxyacetic acid, benzyl amino purin and cupric sulphate on in vitro propagation system from shoot apices of shoot tiller of hybrid Napier grass (Pennisetum purpureum Schum)

An efficient micropropagation method of hybrid Napier grass (Pennisetum purpureumn Schum) for in vitro plant production and material breeding was established from multiple-shoot clumps (MCS) regeneration system. This system was important for forage breeding system. Shoot apices from shoot-tillers produced MSC on Murashige-Skoog (MS) induction medium containing several combinations of BAP and 2,4-D in induction stage. The addition of 5 μM (v/v) and 50 μM (v/v) CuSO4 were added in best medium for inoculation to proliferate the clump in proliferation/multiplication stage. Plant regeneration was achieved by culturing on solid MS with several combination of medium containing NAA and BAP in regeneration stage. The best results for induction were Murashige-Skoog (MS) induction medium containing 2 mgL−1 BAP and 0.1 mgL−1 2,4-D. The proliferation stage on MS medium containing 5 μM CuSÜ4 effective for proliferation (50% multiple shoot formation). The regeneration stage using 0.1 mgL−1 NAA and 2.0 mgL−1 BAP (51.6% number of shoot can regenerate). All plantlets were successfully grown up in an acclimatization stage. Based on the results, the hybrid Napier grass regeneration via MSC was a stable tissue culture system (no albino plats), which could be applied either for further genetic transformation assay or for alternative supply of nursery plant in the future.

Peran Organisasi Sayap Partai Politik dalam Kaderisasi Partai Politik di Indonesia

The political parties that emerge in Indonesia have a distinct catch-all character; they are dependent on individual figures and lack a defined socioeconomic foundation. For political parties, the regeneration process has become a struggle. Because certain political parties lack a clear regeneration system, oligarchic recruiting is a common occurrence. Religious affiliations, local links, local commonalities, and proximity to political party leaders all have a role in recruitment trends. This research aims to explain the role of the under bow of the political party on regeneration. This is a legal-normative study that relies on secondary data. The research material is divided into three categories: primary, secondary, and tertiary. The following factors, according to this study, influence political party regeneration and recruitment: 1) political dynasty has an impact on unhealthy regeneration; 2) political dowry has an impact on unhealthy competition among political party members; and 3) popularity factor has an impact on how quickly people can become political party members. Political parties' inability to recruit and regenerate has an impact on their capacity to fulfill their role as the primary source of national leadership selection. Alternative solutions to these problems include strengthening the under bow of political parties. The existence of under bow of political parties will aid in the transmission of political party doctrine. Political parties will be rewarded with the best members who will fight for their vision and goals. Furthermore, the under bow of political parties can serve as educational institutions for potential members before they join the party.

Principal Modernization Solutions for a 300 MW Power Unit to be Converted to Operate at Ultra-Supercritical Steam Parameters

This paper analyses the state of power engineering in Ukraine and the main trends in the development of the world market in the field of converting high-capacity powerful power units of thermal power plants into ultra-supercritical (USC) ones. It is shown that the energy sector of Ukraine requires special attention and the introduction of new modern technical solutions. Worldwide trends indicate that the emphasis is now on increasing the steam parameters before a turbine to ultra-supercritical ones. This allows one both to increase the efficiency of power units and to reduce thermal emissions, fighting the global environmental problem of climate warming. The implementation of this approach is proposed taking into account the realities of the Ukrainian economy and the available technical capabilities of the power engineering industry. This paper presents the results of variational computational studies of the thermal scheme of the 300 MW power unit of the K-300-23.5 turbine to be converted into a USC one. The problem was solved under the condition of maximizing the preservation of the thermal scheme, increasing the efficiency of the power unit and minimizing capital investments during the modernization of the turbine. It was chosen to preserve the regeneration system, as well as the medium-pressure (MP) and low-pressure (LP) cylinders. Considered and calculated were variants with the addition to the existing turbine of a USC cylinder and the creation of a new high-pressure cylinder (HPC) with insignificant changes in its overall characteristics. The results of computational studies showed that the most rational variant for modernizing the 300 MW turbine plant is the creation of a new HPC designed for operation at USC steam parameters as well as the addition to the IPC of a new cylinder with the purpose of increasing the reheat steam parameters while preserving the regeneration system.

Establishment of Tissue Culture Regeneration System for Medicago ruthenica L. cv. ‘Zhilixing’

Background: Medicago ruthenica L. ‘Zhilixing’ is a new variety with superior forage and seed yield compared to the wild type. The cold, drought and salt tolerance of Zhlixing are better than those of alfalfa, suggesting that this variety can serve as a high-quality genetic resource for improving the stress resistance of alfalfa. However, because of the lack of tissue culture regeneration system, it is difficult to perform genetic transformation studies on stress resistance genes. This study aimed to establish an efficient tissue culture regeneration system for Zhilixing variety. Methods: Three types of explants were selected and tested on four types of basal media supplemented with different combinations of auxin and cytokinin for callus induction and differentiation, based on orthogonal tests to select the combinations of auxin and cytokinin suitable for callus induction and differentiation. Two-factor combination method was used to formulate a suitable rooting medium. Result: The hypocotyledonary axis was found to be an excellent explant for callus induction on MS medium. The optimum callus induction medium contained thidiazuron (TDZ, 0.5 mg/L), 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/L) and naphthaleneacetic acid (NAA, 0.5 mg/L) where the callus induction rate was 93.33%. The differentiation medium was supplemented with TDZ (0.75 mg/L), 2,4-D (0.25 mg/L) and 6-benzyladenine (6-BA, 1.5 mg/L) where the differentiation rate was 63.33%. Thidiazuron played the key role in both processes of callus induction and differentiation. Half-strength MS containing 0.1 mg/L of NAA was the most efficient rooting medium.

Numerical investigation on adsorbent particle erosion characteristic of Y-shaped pipeline in S Zorb regeneration system with different influencing parameters

As the transportation pipeline of adsorbent in S Zorb regeneration system, due to large mass flow rate of particle and gas, it is often seriously worn, and even perforated, resulting in the leakage of adsorbent. It brings great inconvenience to the transportation of regenerated adsorbent and threats its long-term operation. Therefore, it is essential to study the erosion characteristic of Y-shaped adsorbent transportation pipe. It was studied by computational fluid dynamics (CFD) numerical simulation and the calculation results were validated by actual erosion profile. The results show that the high velocity of nitrogen and the high weight-fraction adsorbent particles accumulation is the key factor leading to severe erosion. It is also found that the small angle cutting is the erosion form. The effects of the intersection angle between the transportation pipe and feed pipe and the gas inlet velocity on the particle motion and erosion characteristics turn out to be significant. The research on the wear characteristics of adsorbent transportation pipeline can provide a certain reference for erosion prevention and optimization.

Regeneration potential of different explants during micropropagation of neem tree (Azadirachta indica A. Juss.)

Azadirachta indica A. Juss., (Neem), a prodigious multipurpose tree, has immense potential to benefit mankind and to protect the environment. In order to investigate the effects of three different explants for its regeneration potential, de embryonated cotyledon, immature zygotic embryo and nodal segments from a 30 year old neem plus tree were used. Half strength MS medium with benzyl amino purine (3 mg/L) and naphthalene acetic acid (0.5 mg/L) and casein hydrolysate (1 g/L) was effective in shoot bud sprouting from both nodes and cotyledons. Half strength MS medium fortified with TDZ (0.2 mg/L) was effective for induction of somatic embryogenesis from zygotic embryos. Shoot buds initiated from the cotyledons produced a maximum number of shoots per explants (4.33) which on further sub culturing induced maximum multiple shoots (15) on half strength MS medium fortified with BAP (1.5 mg/L), NAA (0.5 mg/L) and CH (400 g /L) and the nodal explants induced only 4-5 axillary shoots on further sub culturing. Even though immature zygotic embryos produced more number of somatic embryos per explant (24.97) within a short time (30-45 days), the plantlet conversion was poor (25.52 %). In vitro rooting was observed in half strength MS medium supplemented with IBA (2 mg/L). The regeneration potential of de embryonated cotyledons through a simple regeneration system may be beneficial for efficient mass propagation of selected plus trees of neem.

Efficient Plant Regeneration System for New Guinea Impatiens (Impatiens Hawkeri W. Bull) CV. ‘Violet’and ‘Scarlet Bronze Leaf’

New Guinea Impatiens (Impatiens hawkeri W. Bull) is an eye-popping landscaping plant which is of bright and colorful blooms. A highly efficient in vitro plant regeneration system through direct shoot organogenesis was established for the first time from hypocotyl with partial cotyledons of New Guinea Impatiens. The results showed that Explant sterilization method, basic medium type,AgNO3,sucrose and plant growth regulators (PGRs) have greatly influences on in vitro morphogenesis.The regeneration rate in regeneration media that MS supplemented with 0.5mg·L−1 TDZ and 0.1mg·L−1 NAA was acceptable ,the induction rate of 'Violet' was 86.67%, and its proliferation coefficient was 5.27, while the induction rate of 'Scarlet Bronze Leaf' was 83.33%, and its proliferation coefficient was 5.13.PIC was unable to induce clumped sprouts, but it had a better effect on callus induction.We also included a shoot multiplication stage using regeneration New Guinea Impatiens medium that MS supplemented with 0.8mg·L−1 6-BA,0.5mg·L−1 TDZ and 0.05mg ·L−1 NAA.Reducing sucrose concentration to 20g·L−1 or adding 1mg·L−1AgNO3 could alleviate the vitrification phenomenon in the process of tufted bud proliferation.The optimal root culture medium for the regenerated seedlings of 'violet' and 'scarlet bronze leaf' of New Guinea Impatiens was MS supplemented with 0.05mg·L−1IBA, the rooting rate reached 100%.The study examined the micropropagation responses of New Guinea Impatiens in the presence of various growth regulators and provided a simple and more suitable protocol adapted for the mass propagation of clones.