Phenylalanine 171 is a molecular brake for translesion synthesis across benzo[a]pyrene-guanine adducts by human DNA polymerase kappa

2011 ◽  
Vol 718 (1-2) ◽  
pp. 10-17 ◽  
Author(s):  
Akira Sassa ◽  
Naoko Niimi ◽  
Hirofumi Fujimoto ◽  
Atsushi Katafuchi ◽  
Petr Grúz ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Translesion Synthesis ◽  
Human Dna ◽  
Guanine Adducts ◽  
Molecular Brake

scholarly journals DNA binding properties of human DNA polymerase η: implications for fidelity and polymerase switching of translesion synthesis

2004 ◽  
Vol 9 (12) ◽  
pp. 1139-1150 ◽  
Author(s):  
Rika Kusumoto ◽  
Chikahide Masutani ◽  
Shizu Shimmyo ◽  
Shigenori Iwai ◽  
Fumio Hanaoka
Keyword(s):  
Dna Binding ◽  
Dna Polymerase ◽  
Translesion Synthesis ◽  
Binding Properties ◽  
Human Dna ◽  
Dna Binding Properties

scholarly journals Translesion Synthesis past Acrolein-derived DNA Adduct, γ-Hydroxypropanodeoxyguanosine, by Yeast and Human DNA Polymerase η

2002 ◽  
Vol 278 (2) ◽  
pp. 784-790 ◽  
Author(s):  
Irina G. Minko ◽  
M. Todd Washington ◽  
Manorama Kanuri ◽  
Louise Prakash ◽  
Satya Prakash ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Translesion Synthesis ◽  
Dna Adduct ◽  
Human Dna

Translesion Synthesis by Human DNA Polymerase κ on a DNA Template Containing a Single Stereoisomer of dG-(+)- or dG-(−)-anti-N2-BPDE (7,8-Dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene)†

Biochemistry ◽  
2002 ◽  
Vol 41 (19) ◽  
pp. 6100-6106 ◽  
Author(s):  
Naomi Suzuki ◽  
Eiji Ohashi ◽  
Alexander Kolbanovskiy ◽  
Nicholas E. Geacintov ◽  
Arthur P. Grollman ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Translesion Synthesis ◽  
Human Dna ◽  
Dna Template ◽  
Single Stereoisomer

scholarly journals Error-prone Translesion Synthesis by Human DNA Polymerase η on DNA-containing Deoxyadenosine Adducts of 7,8-Dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene

2005 ◽  
Vol 280 (48) ◽  
pp. 39684-39692 ◽  
Author(s):  
Dominic Chiapperino ◽  
Mangmang Cai ◽  
Jane M. Sayer ◽  
Haruhiko Yagi ◽  
Heiko Kroth ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Translesion Synthesis ◽  
Human Dna ◽  
Deoxyadenosine Adducts

scholarly journals Structural basis of DNA synthesis opposite 8-oxoguanine by human PrimPol primase-polymerase

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Olga Rechkoblit ◽  
Robert E. Johnson ◽  
Yogesh K. Gupta ◽  
Louise Prakash ◽  
Satya Prakash ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Thermodynamic Stability ◽  
Active Site ◽  
Base Pair ◽  
Translesion Synthesis ◽  
Human Cells ◽  
Structural Basis ◽  
Human Dna ◽  
Dna Template

AbstractPrimPol is a human DNA polymerase-primase that localizes to mitochondria and nucleus and bypasses the major oxidative lesion 7,8-dihydro-8-oxoguanine (oxoG) via translesion synthesis, in mostly error-free manner. We present structures of PrimPol insertion complexes with a DNA template-primer and correct dCTP or erroneous dATP opposite the lesion, as well as extension complexes with C or A as a 3′−terminal primer base. We show that during the insertion of C and extension from it, the active site is unperturbed, reflecting the readiness of PrimPol to accommodate oxoG(anti). The misinsertion of A opposite oxoG(syn) also does not alter the active site, and is likely less favorable due to lower thermodynamic stability of the oxoG(syn)•A base-pair. During the extension step, oxoG(syn) induces an opening of its base-pair with A or misalignment of the 3′-A primer terminus. Together, the structures show how PrimPol accurately synthesizes DNA opposite oxidatively damaged DNA in human cells.

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