Integration of micronucleus tests with a gene mutation assay in F344 gpt delta transgenic rats using benzo[a]pyrene

2019 ◽  
Vol 837 ◽  
pp. 1-7 ◽  
Author(s):  
Hisako Hori ◽  
Satomi Shimoyoshi ◽  
Yasuhiro Tanaka ◽  
Ayaka Momonami ◽  
Kenichi Masumura ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Transgenic Rats ◽  
Micronucleus Tests ◽  
Mutation Assay ◽  
Gene Mutation Assay ◽  
Gpt Delta

Studies of the Toxicological Potential of Capsinoids: V. Genotoxicity Studies of Dihydrocapsiate

2008 ◽  
Vol 27 (3_suppl) ◽  
pp. 59-72 ◽  
Author(s):  
Bruce K. Bernard ◽  
Eri Watanabe ◽  
Terutaka Kodama ◽  
Shoji Tsubuku ◽  
Akira Otabe ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Chromosomal Aberration ◽  
Metabolic Activation ◽  
Transgenic Rats ◽  
Negative Results ◽  
Chromosomal Aberration Test ◽  
Mutation Assay ◽  
Gene Mutation Assay

A series of studies was performed to evaluate the safety of dihydrocapsiate (4-hydroxy-3-methoxybenzyl 8-methylnonanoate; CAS no. 205687-03-2). This study evaluated the potential genotoxicity of this compound using a variety of in vitro and in vivo test systems, including bacterial reverse mutation test, chromosomal aberration test, micronucleus test, gene mutation assay with transgenic rats, and single-cell gel (SCG) assay (Comet assay). In vitro tests (bacterial reverse mutation test and chromosomal aberration test) produced positive results in the absence of metabolic activation, but negative results in the presence of metabolic activation. The in vivo gene mutation assay (with transgenic rats) produced negative results, as did the in vivo mouse micronucleus assay, which failed to induce micronucleated polychromatic erythrocytes. Although the rat SCG assay produced statistically significant increases in the Olive tail moment and % tail DNA of the liver and intestine in the 2000 mg/kg group (compared with the negative-control group), a number of factors caused the authors to question the validity of these findings. Taken together, these results suggest that dihydrocapsiate has a low or extremely low likelihood of inducing genotoxicity.

scholarly journals The in vivo Pig-a gene mutation assay is applied to study the genotoxicity of procarbazine hydrochloride in Sprague-Dawley rats

2016 ◽  
Vol 3 (4) ◽  
pp. 167-175 ◽  
Author(s):  
Jiang Pu ◽  
Yuanyuan Deng ◽  
Xiaoyan Tan ◽  
Gaofeng Chen ◽  
Cong Zhu ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Mutation Assay ◽  
Gene Mutation Assay

scholarly journals Gene mutation assay using a MutS protein-modified electrode

2002 ◽  
Vol 2 (1) ◽  
pp. 287-288 ◽  
Author(s):  
A. Han ◽  
T. Shibata ◽  
T. Takarada ◽  
M. Maeda
Keyword(s):  
Gene Mutation ◽  
Modified Electrode ◽  
Mutation Assay ◽  
Gene Mutation Assay

scholarly journals Evaluation for a Mutagenicity of 4,4^|^prime;-Methylenedianiline on Hematopoietic Cells by a Pig-a Gene Mutation Assay in Rats

2014 ◽  
Vol 36 (4) ◽  
pp. 179-185 ◽  
Author(s):  
Hisakazu Sanada ◽  
Minako Okamoto ◽  
Tomoka Ohsumi ◽  
Toshiyuki Nakamura
Keyword(s):  
Gene Mutation ◽  
Hematopoietic Cells ◽  
Mutation Assay ◽  
Gene Mutation Assay

Germ Cell Gene Mutation Assay

2017 ◽  
Author(s):  
John H. Duffus ◽  
Michael Schwenk ◽  
Douglas M. Templeton
Keyword(s):  
Germ Cell ◽  
Gene Mutation ◽  
Mutation Assay ◽  
Cell Gene ◽  
Gene Mutation Assay
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