scholarly journals Gastric Electrical Stimulation Increases the Proliferation of Interstitial Cells of Cajal and Alters the Enteric Nervous System in Diabetic Rats

2022 ◽  
Author(s):  
Yan Chen ◽  
Shuhui Zhang ◽  
Ying Li ◽  
Hui Yan ◽  
Ying Ba ◽  
...  
Keyword(s):  
Nervous System ◽  
Electrical Stimulation ◽  
Enteric Nervous System ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Rats ◽  
Gastric Electrical Stimulation ◽  
Cells Of Cajal

scholarly journals Gastric Electrical Stimulation Increases The Proliferation of Interstitial Cells of Cajal and Alters Enteric Nervous System in Diabetic Rats

2020 ◽  
Author(s):  
Cai Wang ◽  
Hui Zhang ◽  
Le Zhao ◽  
Tao Gao ◽  
Xia Liu ◽  
...  
Keyword(s):  
Nervous System ◽  
Electrical Stimulation ◽  
Enteric Nervous System ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Rats ◽  
Gastric Electrical Stimulation ◽  
Enteric Neurons ◽  
Cells Of Cajal ◽  
The Impact

Abstract Background: Lack of interstitial cells of Cajal (ICC) and neuropathy were the most possible pathological mechanisms of diabetic gastroparesis. Gastric electrical stimulation (GES) is a promising way to treat gastroparesis. The aims of the present study were to explore the impact of GES on ICC together with enteric neurons in diabetic rats and the possible mechanisms involved.Methods: Sixty rats were randomized into the normal rats, diabetic rats (DM), diabetic rats with sham GES (DM+SGES), and three diabetic rats with GES (DM+GES1, DM+GES2 and DM+GES3). The proliferation of ICC and expressions of 5-HT2B, nNOS, CHAT, PGP9.5 and GDNF were evaluated by immunofluorescence staining or Western blot. The expressions of 5-HT in blood and tissue were determined by ELISA.Results: (1) The proliferation of ICC was hardly observed in the DM group together with the DM+SGES group but increased in the three DM+GES groups. (2) The expression of 5-HT2B was decreased in the DM group and enhanced in the DM+GES groups. Similarly, the expressions of 5-HT in the blood and distal stomach tissue were increased in the DM+GES groups. (3) Both nNOS labeled neurons and CHAT positive neurons were reduced in myenteric plexus of the DM group, while plenty of these neurons were observed the DM+GES groups. (4) The expression of GDNF protein in the diabetic rats was down-regulated, while GES increased the expression of GDNF.Conclusion: GES improves the proliferation of ICC possibly related with 5-HT/5-HT2B signal pathway, and alters enteric nervous system 52 partly though the GDNF expression.

scholarly journals Neurons and a Subset of Interstitial Cells of Cajal in the Enteric Nervous System Highly Express Stam2 Gene

2011 ◽  
Vol 295 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Katarina Kapuralin ◽  
Chris Van Ginneken ◽  
Marija Curlin ◽  
Jean-Pierre Timmermans ◽  
Srecko Gajovic
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal

scholarly journals Abnormalities of the intestinal pacemaker cells, enteric neurons, and smooth muscle in intestinal atresia

2019 ◽  
Vol 11 (03) ◽  
pp. 180-185 ◽  
Author(s):  
Radhika krishna OH ◽  
Mohammed Abdul Aleem ◽  
Geetha Kayla
Keyword(s):  
Nervous System ◽  
Small Bowel ◽  
Enteric Nervous System ◽  
Gastrointestinal Motility ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Enteric Neurons ◽  
Small Bowel Atresia ◽  
Bowel Atresia ◽  
Cells Of Cajal

Abstract BACKGROUND: Small bowel atresia is a congenital disorder that carves a substantial morbidity. Numerous postoperative gastrointestinal motility problems occur. The underlying cause of this motility disorder is still unclear. Interstitial cells of Cajal (ICC) play a major role in gastrointestinal motility. AIMS AND OBJECTIVES: To investigate the morphological changes of enteric nervous system and ICC in small bowel atresia. MATERIAL AND METHODS: Resected small bowel specimen from affected patients (n=15) were divided into three parts (proximal, distal, atretic). Standard histology and immunohistochemistry with anti C-KIT receptor antibody (CD117), calretinin and α-SMA was carried out. The density of myenteric ICCs in the proximal, atretic and distal parts was demonstrated by CD 117 while Calretinin was used for ganglion cells and nerve bundles, α-SMA highlighted muscle hypertrophy. RESULT AND CONCLUSION: The proximal and distal bowel revealed clear changes in the morphology and density of enteric nervous system and interstitial cells of Cajal..

Development of interstitial cells of Cajal in a full-term infant without an enteric nervous system

2001 ◽  
Vol 120 (2) ◽  
pp. 561-567 ◽  
Author(s):  
Jan D. Huizinga ◽  
Irene Berezin ◽  
Kanishka Sircar ◽  
Bryan Hewlett ◽  
Graeme Donnelly ◽  
...  
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Term Infant ◽  
Full Term ◽  
Full Term Infant ◽  
Cells Of Cajal

Innervation of interstitial cells of Cajal by vasoactive intestinal polypeptide containing nerves in canine colon

1990 ◽  
Vol 68 (7) ◽  
pp. 922-932 ◽  
Author(s):  
Irene Berezin ◽  
Jan D. Huizinga ◽  
Laura Farraway ◽  
Edwin E. Daniel
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Vasoactive Intestinal Polypeptide ◽  
Interstitial Cells Of Cajal ◽  
Circular Muscle ◽  
Interstitial Cells ◽  
Granular Vesicles ◽  
Cells Of Cajal ◽  
Large Granular Vesicles ◽  
Intestinal Polypeptide

The hypothesis was tested, through structural and functional studies, that interstitial cells of Cajal receive and can respond to direct innervation from nerves containing the vasoactive intestinal polypeptide neuromediator. The submucosal network of interstitial cells of Cajal has been postulated to provide pacemaking activity for the circular muscle and to be involved in neurotransmission from noradrenergic, noncholinergic nerves for which vasoactive intestinal polypeptide is a putative mediator. The distribution of vasoactive intestinal polypeptide and substance P immunoreactive material in nerve profiles of the enteric nervous system of the canine colon was examined. In addition, electrophysiological studies were done on the interstitial cells bordering the submucosal side of the circular muscle layer after they were electrically isolated using heptanol. The vasoactive intestinal polypeptide immunoreactivity, located exclusively in nerve large granular vesicles, was found throughout the enteric nervous system (myenteric plexus, submucous plexus, and circular muscle – submucosa interface). The highest proportion (38% compared with 22–24%) of profiles of large granular vesicles with vasoactive intestinal polypeptide immunoreactivity was found in nerve profiles of the circular muscle – submucosa interface. In contrast, substance P immunoreactivity was found in nerve profiles of myenteric plexus (33% of large granular vesicles were positive) but not associated with submucosal interstitial cell nerve network. The vasoactive intestinal polypeptide hyperpolarized interstitial cells by 9 mV when electrically isolated by 1 mM heptanol and markedly reduced (about 50%) their input membrane resistance. We conclude that the distribution of vasoactive intestinal polypeptide immunoreactivity and its action are consistent with a postulated role of the interstitial cells as a major site of neurally mediated inhibition of colonic pacemaker activity.Key words: enteric nervous system, interstitial cells of Cajal, inhibitory junction potential, nonadrenergic noncholinergic nerves.

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