Remodelling of synaptic morphology but unchanged synaptic density during late phase long-term potentiation(ltp): A serial section electron micrograph study in the dentate gyrus in the anaesthetised rat

Neuroscience ◽  
2004 ◽  
Vol 128 (2) ◽  
pp. 251-262 ◽  
Author(s):  
V.I. Popov ◽  
H.A. Davies ◽  
V.V. Rogachevsky ◽  
I.V. Patrushev ◽  
M.L. Errington ◽  
...  
Keyword(s):  
Dentate Gyrus ◽  
Serial Section ◽  
Late Phase ◽  
Long Term Potentiation ◽  
Electron Micrograph ◽  
Synaptic Density ◽  
Term Potentiation ◽  
Section Electron ◽  
Anaesthetised Rat

scholarly journals Ultrastructure of light-activated axons following optogenetic stimulation to produce late-phase long-term potentiation

2019 ◽  
Author(s):  
Masaaki Kuwajima ◽  
Olga I. Ostrovskaya ◽  
Guan Cao ◽  
Seth A. Weisberg ◽  
Kristen M. Harris ◽  
...  
Keyword(s):  
Late Phase ◽  
Long Term Potentiation ◽  
State Dependent ◽  
Term Potentiation ◽  
Section Electron ◽  
Hippocampal Ca3 ◽  
Ultrastructure And Function ◽  
Ca3 Neurons ◽  
And Function

AbstractAnalysis of neuronal compartments has revealed many state-dependent changes in geometry but establishing synapse-specific mechanisms at the nanoscale has proven elusive. We co-expressed channelrhodopsin2-GFP and mAPEX2 in a subset of hippocampal CA3 neurons and used trains of light to induce late-phase long-term potentiation (L-LTP) in area CA1. L-LTP was shown to be specific to the labeled axons by severing CA3 inputs, which prevented back-propagating recruitment of unlabeled axons. Membrane-associated mAPEX2 tolerated microwave-enhanced chemical fixation and drove tyramide signal amplification to deposit Alexa Fluor dyes in the light-activated axons. Subsequent post-embedding immunogold labeling resulted in outstanding ultrastructure and clear distinctions between labeled (activated), and unlabeled axons without obscuring subcellular organelles. The gold-labeled axons in potentiated slices were reconstructed through serial section electron microscopy; presynaptic vesicles and other constituents could be quantified unambiguously. The genetic specification, reliable physiology, and compatibility with established methods for ultrastructural preservation make this an ideal approach to link synapse ultrastructure and function in intact circuits.

Spine formation pattern of new neurons is modulated by induction of long-term potentiation (LTP) in adult dentate gyrus

2009 ◽  
Vol 65 ◽  
pp. S83
Author(s):  
Noriaki Ohkawa ◽  
Yoshito Saitoh ◽  
Eri Tokunaga ◽  
Toshio Kitamura ◽  
Kaoru Inokuchi
Keyword(s):  
Dentate Gyrus ◽  
Long Term Potentiation ◽  
Spine Formation ◽  
Term Potentiation ◽  
Formation Pattern

Spatial performance correlates with long-term potentiation of the dentate gyrus but not of the CA1 region in rats with fimbria–fornix lesions

2001 ◽  
Vol 307 (3) ◽  
pp. 159-162 ◽  
Author(s):  
Kazuhito Nakao ◽  
Yuji Ikegaya ◽  
Maki K Yamada ◽  
Nobuyoshi Nishiyama ◽  
Norio Matsuki
Keyword(s):  
Dentate Gyrus ◽  
Long Term Potentiation ◽  
Ca1 Region ◽  
Term Potentiation ◽  
Spatial Performance
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