Purification of a recombinant membrane protein tagged with a calmodulin-binding domain: properties of chimeras of the Escherichia coli nicotinamide nucleotide transhydrogenase and the C-terminus of human plasma membrane Ca2+-ATPase

2004 ◽  
Vol 36 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Maxim V Egorov ◽  
Anna Tigerström ◽  
Nikolay B Pestov ◽  
Tatyana V Korneenko ◽  
Maria B Kostina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plasma Membrane ◽  
Membrane Protein ◽  
Human Plasma ◽  
Binding Domain ◽  
Nicotinamide Nucleotide Transhydrogenase ◽  
Calmodulin Binding ◽  
C Terminus

scholarly journals The Calmodulin Binding Domain of the Plasma Membrane Ca2+ Pump Interacts Both with Calmodulin and with Another Part of the Pump

1989 ◽  
Vol 264 (21) ◽  
pp. 12313-12321 ◽  
Author(s):  
A Enyedi ◽  
T Vorherr ◽  
P James ◽  
D J McCormick ◽  
A G Filoteo ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Binding Domain ◽  
Calmodulin Binding

Interaction of calmodulin with the calmodulin binding domain of the plasma membrane calcium pump

Biochemistry ◽  
1990 ◽  
Vol 29 (2) ◽  
pp. 355-365 ◽  
Author(s):  
Thomas Vorherr ◽  
Peter James ◽  
Joachim Krebs ◽  
Agnes Enyedi ◽  
Daniel J. McCormick ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Binding Domain ◽  
Calcium Pump ◽  
Calmodulin Binding ◽  
Plasma Membrane Calcium Pump

scholarly journals The large intracellular loop of hZIP4 is an intrinsically disordered zinc binding domain

Metallomics ◽  
2015 ◽  
Vol 7 (9) ◽  
pp. 1319-1330 ◽  
Author(s):  
Elizabeth M. Bafaro ◽  
Sagar Antala ◽  
Tuong-Vi Nguyen ◽  
Stephen P. Dzul ◽  
Brian Doyon ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Protein ◽  
Binding Domain ◽  
Zinc Binding ◽  
Plasma Membrane Protein ◽  
Intracellular Loop ◽  
Intrinsically Disordered ◽  
Zinc Binding Domain

The human (h) ZIP4 transporter is a plasma membrane protein which functions to increase the cytosolic concentration of zinc.

scholarly journals Protein Kinase C Phosphorylates Plasma Membrane Ca2+Pump Isoform 4a at Its Calmodulin Binding Domain

1999 ◽  
Vol 274 (1) ◽  
pp. 527-531 ◽  
Author(s):  
Anil K. Verma ◽  
Katalin Paszty ◽  
Adelaida G. Filoteo ◽  
John T. Penniston ◽  
Agnes Enyedi
Keyword(s):  
Plasma Membrane ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Binding Domain ◽  
Calmodulin Binding ◽  
Kinase C

scholarly journals New Ca2+ pump isoforms generated by alternative splicing of rPMCA2 mRNA

1992 ◽  
Vol 283 (2) ◽  
pp. 355-359 ◽  
Author(s):  
H P Adamo ◽  
J T Penniston
Keyword(s):  
Amino Acids ◽  
Variable Region ◽  
Rat Plasma ◽  
Lipid Binding ◽  
Binding Domain ◽  
Kinase Substrate ◽  
Calmodulin Binding ◽  
Hypervariable Regions ◽  
C Terminus ◽  
Domain Insertion

Alternative splices capable of generating proteins with altered functions were found (by PCR) in isoform 2 of the rat plasma membrane Ca2+ pump. These splices were concentrated in two hypervariable regions. One of these regions, near the N-terminus and the lipid-binding region, could be altered by the insertion of either or both of inserts x and y. Insertion of both x and y would add 45 amino acids to the molecule. The y insert causes the appearance of a rather hydrophobic stretch of amino acids in the middle of a highly polar region. The second variable region begins in the middle of the calmodulin-binding domain. Insertion of 229 nucleotides at this point of the message converts the b form to the a form, which has an altered (and shorter) C-terminus. The calmodulin-binding domain of this shortened form has a less basic character, which would decrease the affinity for calmodulin. The b form of isoenzyme 2 contains relatively weak protein kinase A substrate sequences, such as KQNSS and KNNS. These sequences are eliminated in form a, and a strongly activated kinase substrate sequence, RRQSS, appears in a different place. Different tissues use different combinations of alternative splices, with heart and brain showing the greatest diversity.

scholarly journals Plasma Membrane Calcium Pump Isoform 4a Has a Longer Calmodulin-Binding Domain Than 4b

1996 ◽  
Vol 271 (7) ◽  
pp. 3714-3718 ◽  
Author(s):  
Anil K. Verma ◽  
Agnes Enyedi ◽  
Adelaida G. Filoteo ◽  
Emanuel E. Strehler ◽  
John T. Penniston
Keyword(s):  
Plasma Membrane ◽  
Binding Domain ◽  
Calcium Pump ◽  
Calmodulin Binding ◽  
Plasma Membrane Calcium Pump
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