Production and characterization of a retinoic acid receptor RARγ construction encompassing the DNA binding domain and the disordered N-terminal proline rich domain

2014 ◽  
Vol 95 ◽  
pp. 113-120 ◽  
Author(s):  
Denise Martinez-Zapien ◽  
Marc-André Delsuc ◽  
Gilles Travé ◽  
Régis Lutzing ◽  
Cécile Rochette-Egly ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Dna Binding ◽  
Retinoic Acid Receptor ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Acid Receptor ◽  
Rich Domain

scholarly journals The solution structure of the human retinoic acid receptor-β DNA-binding domain

1993 ◽  
Vol 3 (1) ◽  
pp. 1-17 ◽  
Author(s):  
R. M. A. Knegtel ◽  
M. Katahira ◽  
J. G. Schilthuis ◽  
A. M. J. J. Bonvin ◽  
R. Boelens ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Dna Binding ◽  
Solution Structure ◽  
Retinoic Acid Receptor ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Acid Receptor

Heteronuclear 113Cd-1H NMR Study of Metal Coordination in the Human Retinoic Acid Receptor-β DNA Binding Domain

1993 ◽  
Vol 192 (2) ◽  
pp. 492-498 ◽  
Author(s):  
R.M.A. Knegtel ◽  
R. Boelens ◽  
M.L. Ganadu ◽  
A.V.E. George ◽  
P.T. Vandersaag ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Dna Binding ◽  
1H Nmr ◽  
Retinoic Acid Receptor ◽  
Binding Domain ◽  
Metal Coordination ◽  
Dna Binding Domain ◽  
Acid Receptor ◽  
Nmr Study

NMR Studies of the Human Retinoic Acid Receptor-? DNA-Binding Domain.

1993 ◽  
Vol 684 (1 Zinc-Finger P) ◽  
pp. 49-62 ◽  
Author(s):  
R. M. A. KNEGTEL ◽  
R. BOELENS ◽  
M. L. GANADU ◽  
A. V. E. GEORGE ◽  
M. KATAHIRA ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Dna Binding ◽  
Retinoic Acid Receptor ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Nmr Studies ◽  
Acid Receptor

scholarly journals Characterization of the Elk-1 ETS DNA-binding Domain

1995 ◽  
Vol 270 (11) ◽  
pp. 5805-5811 ◽  
Author(s):  
Paul Shore ◽  
Louise Bisset ◽  
Jeremy Lakey ◽  
Jonathan P. Waltho ◽  
Richard Virden ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Domain ◽  
Dna Binding Domain

Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1

1992 ◽  
Vol 12 (10) ◽  
pp. 4297-4304 ◽  
Author(s):  
D Ray ◽  
R Bosselut ◽  
J Ghysdael ◽  
M G Mattei ◽  
A Tavitian ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
New Gene ◽  
Carboxy Terminal Region ◽  
Carboxy Terminal ◽  
Hematopoietic Cell Lines

We have cloned a human cDNA from a new gene, spi-B, on the basis of its homology with the DNA-binding domain of the Spi-1/PU.1 putative oncogene product. spi-B codes for a protein of 262 amino acids presenting 43% overall identity with Spi-1. Its highly basic carboxy-terminal region exhibits 34% sequence identity with the DNA-binding domain of the Ets-1 protein. We showed that the Spi-B protein is able to bind the purine-rich sequence (PU box) recognized by Spi-1/PU.1 and to activate transcription of a reporter plasmid containing PU boxes. Chromosome in situ hybridization allowed us to map spi-B to the 19q13.3-19q13.4 region of the human genome. spi-B, like spi-1, was found to be expressed in various murine and human hematopoietic cell lines except T lymphoid cell lines.

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