High-level Extracellular Production and Immobilisation of Methyl Parathion Hydrolase from Plesiomonas sp. M6 Expressed in Pichia pastoris

2021 ◽  
pp. 105859
Author(s):  
Wang Ying ◽  
Wang YaPing ◽  
Huang Can ◽  
Ma Lixin ◽  
Yan Hong ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Methyl Parathion ◽  
Extracellular Production ◽  
Methyl Parathion Hydrolase ◽  
Parathion Hydrolase ◽  
High Level

Expression of Methyl Parathion Hydrolase in Pichia pastoris

2009 ◽  
Vol 59 (6) ◽  
pp. 573-578 ◽  
Author(s):  
Haojie Yu ◽  
Xin Yan ◽  
Weiliang Shen ◽  
Qing Hong ◽  
Ji Zhang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Methyl Parathion ◽  
Methyl Parathion Hydrolase ◽  
Parathion Hydrolase

scholarly journals High-Level Expression and Secretion of Methyl Parathion Hydrolase in Bacillus subtilis WB800

2005 ◽  
Vol 71 (7) ◽  
pp. 4101-4103 ◽  
Author(s):  
Xiao-Zhou Zhang ◽  
Zhong-Li Cui ◽  
Qing Hong ◽  
Shun-Peng Li
Keyword(s):  
Bacillus Subtilis ◽  
Signal Peptide ◽  
Methyl Parathion ◽  
High Level Expression ◽  
Methyl Parathion Hydrolase ◽  
Parathion Hydrolase ◽  
Encoding Gene ◽  
Protease Deficient ◽  
High Level

ABSTRACT The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression and secretion of mature, authentic, and stable MPH were achieved using the protease-deficient strain B. subtilis WB800 as the host.

scholarly journals Improving the Secretion of a Methyl Parathion Hydrolase in Pichia pastoris by Modifying Its N-Terminal Sequence

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96974 ◽  
Author(s):  
Ping Wang ◽  
Lu Huang ◽  
Hu Jiang ◽  
Jian Tian ◽  
Xiaoyu Chu ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Methyl Parathion ◽  
Terminal Sequence ◽  
Methyl Parathion Hydrolase ◽  
Parathion Hydrolase

Crystal Structure of Methyl Parathion Hydrolase from Pseudomonas sp. WBC-3

2005 ◽  
Vol 353 (3) ◽  
pp. 655-663 ◽  
Author(s):  
Yan-Jie Dong ◽  
Mark Bartlam ◽  
Lei Sun ◽  
Ya-Feng Zhou ◽  
Zhi-Ping Zhang ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Methyl Parathion ◽  
Pseudomonas Sp ◽  
Methyl Parathion Hydrolase ◽  
Parathion Hydrolase

Twin-Arginine Translocation of Methyl Parathion Hydrolase inBacillus subtilis

2010 ◽  
Vol 44 (19) ◽  
pp. 7607-7612 ◽  
Author(s):  
Chao Yang ◽  
Cunjiang Song ◽  
Roland Freudl ◽  
Ashok Mulchandani ◽  
Chuanling Qiao
Keyword(s):  
Methyl Parathion ◽  
Methyl Parathion Hydrolase ◽  
Twin Arginine Translocation ◽  
Parathion Hydrolase

Application of methyl parathion hydrolase (MPH) as a labeling enzyme

2008 ◽  
Vol 390 (8) ◽  
pp. 2133-2140 ◽  
Author(s):  
Wei Yang ◽  
Ya-Feng Zhou ◽  
He-Ping Dai ◽  
Li-Jun Bi ◽  
Zhi-Ping Zhang ◽  
...  
Keyword(s):  
Methyl Parathion ◽  
Methyl Parathion Hydrolase ◽  
Parathion Hydrolase

scholarly journals Isolation of Methyl Parathion-Degrading Strain M6 and Cloning of the Methyl Parathion Hydrolase Gene

2001 ◽  
Vol 67 (10) ◽  
pp. 4922-4925 ◽  
Author(s):  
Cui Zhongli ◽  
Li Shunpeng ◽  
Fu Guoping
Keyword(s):  
Nucleotide Sequence ◽  
Methyl Parathion ◽  
Genomic Library ◽  
Methyl Parathion Hydrolase ◽  
Organophosphate Hydrolase ◽  
Shotgun Cloning ◽  
Esherichia Coli ◽  
Parathion Hydrolase

ABSTRACT A degradative bacterium, M6, was isolated and presumptively identified as Plesiomonas sp. strain M6 was able to hydrolyze methyl parathion to p-nitrophenol. A novel organophosphate hydrolase gene designated mpd was selected from its genomic library prepared by shotgun cloning. The nucleotide sequence of the mpd gene was determined. The gene could be effectively expressed in Esherichia coli.

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