Unusual matrine–adenine hybrids isolated from Sophora davidii and their inhibitory effects on human cytomegalovirus

2021 ◽  
Vol 190 ◽  
pp. 112842
Author(s):  
Zhi-Jun Zhang ◽  
Shu-Wei Dong ◽  
Dan-Dan Gao ◽  
Xin-Ye Du ◽  
Yan-Qing Xie ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Inhibitory Effects ◽  
Sophora Davidii

scholarly journals Recombinant Green Fluorescent Protein-Expressing Human Cytomegalovirus as a Tool for Screening Antiviral Agents

2000 ◽  
Vol 44 (6) ◽  
pp. 1588-1597 ◽  
Author(s):  
Manfred Marschall ◽  
Martina Freitag ◽  
Sigrid Weiler ◽  
Gabriele Sorg ◽  
Thomas Stamminger
Keyword(s):  
Green Fluorescent Protein ◽  
Human Cytomegalovirus ◽  
Fluorescent Protein ◽  
Antiviral Agents ◽  
Inhibitory Effects ◽  
Plaque Reduction Assay ◽  
Human Fibroblast Cultures ◽  
Antiviral Activities ◽  
Green Fluorescent

ABSTRACT A recombinant human cytomegalovirus (AD169-GFP) expressing green fluorescent protein was generated by homologous recombination. Infection of human fibroblast cultures with AD169-GFP virus produced stable and readily detectable amounts of GFP signals which were quantitated by automated fluorometry. Hereby, high levels of sensitivity and reproducibility could be achieved, compared to those with the conventional plaque reduction assay. Antiviral activities were determined for four reference compounds as well as a set of putative novel cytomegalovirus inhibitors. The results obtained were exactly in line with the known characteristics of reference compounds and furthermore revealed distinct antiviral activities of novel in vitro inhibitors. The fluorometric data could be confirmed by GFP-based flow cytometry and fluorescence microscopy. In addition, laboratory virus variants derived from the recombinant AD169-GFP virus provided further possibilities for study of the characteristics of drug resistance. The GFP-based antiviral assay appeared to be very reliable for measuring virus-inhibitory effects in concentration- and time-dependent fashions and might also be adaptable for high-throughput screenings of cytomegalovirus-specific antiviral agents.

Inhibitory Effects of Statins on Expression of Immediate–Early 1 Protein of Human Cytomegalovirus in Virus-infected Cells

2013 ◽  
Vol 5 (5) ◽  
pp. 187-193 ◽  
Author(s):  
Hidetaka Sadanari ◽  
Tsugiya Murayama ◽  
Xin Zheng ◽  
Rie Yamada ◽  
Keiko Matsubara ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Immediate Early ◽  
Inhibitory Effects ◽  
Infected Cells

scholarly journals Binding of human cytomegalovirus to sulfated glucuronyl glycosphingolipids and their inhibitory effects on the infection.

1998 ◽  
Vol 79 (10) ◽  
pp. 2533-2541 ◽  
Author(s):  
K Ogawa-Goto ◽  
Y Arao ◽  
H Akanuma ◽  
T Abe ◽  
S Irie ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Inhibitory Effects

Inhibitory effects of piceatannol on human cytomegalovirus (hCMV) in vitro

2020 ◽  
Vol 58 (8) ◽  
pp. 716-723
Author(s):  
San-Ying Wang ◽  
Jing Zhang ◽  
Xiao-Gang Xu ◽  
Hui-Li Su ◽  
Wen-Min Xing ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Inhibitory Effects

Inhibitory effects of tricin derivative from Sasa albo-marginata on replication of human cytomegalovirus

2011 ◽  
Vol 91 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Kazuhiko Akuzawa ◽  
Rie Yamada ◽  
Zhuan Li ◽  
Ying Li ◽  
Hidetaka Sadanari ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Inhibitory Effects
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