The investigation on the sensitivity of the flows to the initial conditions was carried with varying grid resolutions, turbulence models and perturbations. The Direct Numerical Simulation study of Pantano and Sarkar [1] was taken as the reference study and the computational model used in this study was built with a similar configuration of theirs except for the perturbations used. Important results were arrived pertaining to the effect of initial conditions on the turbulent properties and the turbulent structures of the flow.