scholarly journals Characterization of endothelial thromboxane receptors in rabbit aorta

2008 ◽  
Vol 87 (1-4) ◽  
pp. 54-61 ◽  
Author(s):  
Sandra L. Pfister
Keyword(s):  
Rabbit Aorta ◽  
Thromboxane Receptors

Biochemical Characterization of Elastin in Neointimal Hyperplasiaof Rabbit Aorta

Matrix ◽  
1989 ◽  
Vol 9 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Madeleine Moczar ◽  
Sören Björkerud ◽  
Ladislas Robert
Keyword(s):  
Biochemical Characterization ◽  
Rabbit Aorta

Characterization of a continuous smooth muscle cell line derived from rabbit aorta

1989 ◽  
Vol 25 (10) ◽  
pp. 892-898 ◽  
Author(s):  
Maurice Nachtigal ◽  
Madan L. Nagpal ◽  
Phillip Greenspan ◽  
Sidonia A. Nachtigal ◽  
Alain Legrand
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Rabbit Aorta ◽  
Muscle Cell Line

Characterization of the rabbit aorta endothelium-dependent cholinergic receptor by agonist equipotent molar doses

1989 ◽  
Vol 22 (4) ◽  
pp. 219-231 ◽  
Author(s):  
Dan Bradu ◽  
Bernardino Di Sarra ◽  
Carolina Concettoni ◽  
Vincenzo Moretti ◽  
Paolo Pagelli ◽  
...  
Keyword(s):  
Rabbit Aorta ◽  
Cholinergic Receptor

scholarly journals Role of superoxide and thromboxane receptors in acute angiotensin II-induced vasoconstriction of rabbit vessels

2011 ◽  
Vol 300 (6) ◽  
pp. H2064-H2071 ◽  
Author(s):  
Sandra L. Pfister ◽  
Kasem Nithipatikom ◽  
William B. Campbell
Keyword(s):  
Arachidonic Acid ◽  
Angiotensin Ii ◽  
Rabbit Aorta ◽  
Arachidonic Acid Metabolite ◽  
Maximal Contraction ◽  
Nitric Oxide Synthase Inhibitor ◽  
Thromboxane Receptor ◽  
Thromboxane Receptors ◽  
Thromboxane Receptor Antagonist ◽  
Synthase Inhibitor

This study explored the hypothesis that a portion of angiotensin II-induced contractions is dependent on superoxide generation and release of a previously unidentified arachidonic acid metabolite that activates vascular smooth muscle thromboxane receptors. Treatment of rabbit aorta or mesentery artery with the thromboxane receptor antagonist SQ29548 (10 μM) reduced angiotensin II-induced contractions (maximal contraction in aorta; control vs. SQ29548: 134 ± 16 vs. 93 ± 10%). A subset of rabbits deficient in vascular thromboxane receptors also displayed decreased contractions to angiotensin II. The superoxide dismutase mimetic Tiron (30 mM) attenuated angiotensin II-induced contractions only in rabbits with functional vascular thromboxane receptors (maximal contraction in aorta; control vs. Tiron: 105 ± 5 vs. 69 ± 11%). Removal of the endothelium or treatment with a nitric oxide synthase inhibitor, nitro-l-arginine (30 μM) did not alter angiotensin II-induced contractions. Tiron and SQ29548 decreased angiotensin II-induced contractions in the denuded aortas by a similar percentage as that observed in intact vessels. The cyclooxygenase inhibitor indomethacin (10 μM) or thromboxane synthase inhibitor dazoxiben (10 μM) had no effect on angiotensin II-induced contractions indicating that the vasoconstrictor was not thromboxane. Angiotensin II increased the formation of a 15-series isoprostane. Isoprostanes are free radical-derived products of arachidonic acid. The unidentified isoprostane increased when vessels were incubated with the superoxide-generating system xanthine/xanthine oxidase. Pretreatment of rabbit aorta with the isoprostane isolated from aortic incubations enhanced angiotensin II-induced contractions. Results suggest the factor activating thromboxane receptors and contributing to angiotensin II vasoconstriction involves the superoxide-mediated generation of a 15-series isoprostane.

Pharmacological characterization of ATP receptors mediating vasodilation on isolated rabbit aorta

1992 ◽  
Vol 23 (5) ◽  
pp. 861-865 ◽  
Author(s):  
Alessandro Chinellato ◽  
Eugenio Ragazzi ◽  
Luisa Pandolfo ◽  
Guglielmina Froldi ◽  
Laura Caparrotta ◽  
...  
Keyword(s):  
Rabbit Aorta ◽  
Pharmacological Characterization ◽  
Atp Receptors ◽  
Isolated Rabbit Aorta

Characterization of receptors for angiotensin in the rat vas deferens

1979 ◽  
Vol 57 (4) ◽  
pp. 417-423 ◽  
Author(s):  
J. Magnan ◽  
D. Regoli
Keyword(s):  
Smooth Muscle ◽  
Vas Deferens ◽  
Proteolytic Enzymes ◽  
Rabbit Aorta ◽  
Rat Vas Deferens ◽  
Converting Enzyme ◽  
Response Curves ◽  
Sympathetic Nerve Stimulation ◽  
The Right

Experiments were performed in the rat vas deferens to characterize the receptor for angiotensin mediating the potentiation of the sympathetic nerve stimulation by this peptide. For this purpose we measured the order of potency of various angiotensins, the affinity of two specific and competitive antagonists, and we compared the effects of several angiotensins in tissues desensitized by angiotensin II.The potency of natural angiotensins follows the order: ATII > ATI > ATIII the relative potency of a few analogues which resist degradation by proteolytic enzymes as well as the potency of three L-Ala analogues of ATII show similar changes as those observed in other smooth muscle preparations (e.g. the rabbit aorta).Affinity of antagonists was evaluated by measuring pA2 and is higher for [Leu8]-ATII than for [des-Asp1, Leu8]-ATII. Both antagonists appear to be competitive since they displace the dose-response curves of ATII and ATIII to the right without changing the slope of the curves. Desensitization with ATII renders the tissues insensitive to ATIII and to other angiotensins without changing the response of the tissues to substance P.ATII does not modify the action of exogenous NA on nonstimulated tissues. ATI has no direct effect since its action is completely blocked in the presence of an inhibitor of the converting enzyme (SQ. 14225), while the responses of the vas deferens to ATII and substance P are unaltered.It is concluded that the receptor for ATII in the rat vas deferens is of the same type as the receptor mediating contraction of the rabbit aorta.

Pharmacological characterization of a new purinergic receptor site in rabbit aorta

1992 ◽  
Vol 23 (6) ◽  
pp. 1067-1071 ◽  
Author(s):  
Alessandro Chinellato ◽  
Eugenio Ragazzi ◽  
Luisa Pandolfo ◽  
Guglielmina Froldi ◽  
Laura Caparrotta ◽  
...  
Keyword(s):  
Purinergic Receptor ◽  
Receptor Site ◽  
Rabbit Aorta ◽  
Pharmacological Characterization

scholarly journals In situ ultrastructural characterization of chondroitin sulfate proteoglycans in normal rabbit aorta.

1992 ◽  
Vol 40 (2) ◽  
pp. 251-263 ◽  
Author(s):  
Z S Galis ◽  
M Z Alavi ◽  
S Moore
Keyword(s):  
Chondroitin Sulfate ◽  
Rabbit Aorta ◽  
Specific Information ◽  
In Situ Characterization ◽  
Tissue Sections ◽  
Ultrastructural Characterization ◽  
The Media ◽  
Very High

We used a monoclonal antibody recognizing chondroitin sulfate (CS) to investigate by immunocytochemistry the characteristics displayed in situ by aortic proteoglycans (PG) containing CS side chains. The antibody specifically precipitated metabolically labeled PG from aortic extracts. Anti-CS specificity was also tested directly on tissue sections and was confirmed by the virtual abolition of immunolabeling on those previously digested with CS-specific enzymes. The overall CS-PG distribution assessed by light microscopy after embedding in Lowicryl KM4 by silver-enhanced immunogold recapitulated that obtained on frozen sections with immunoperoxidase. Extracellular concentrations of CS-PG were very high in the innermost regions of aorta and decreased in the media. The reaction was weak and diffuse in the adventitia. By electron microscopy, the detailed labeling of CS-PG discriminated patterns of organization at both the regional and the molecular level and enabled morphometric estimations. In relation to other components of the extracellular matrix, we found that CS-PG and elastin mutually excluded each other, while two types of CS-PG were differently associated with collagen within media or adventitia. The use of high-resolution immunodetection for the in situ characterization of aortic CS-PG could add specific information relevant to many biological processes in which these molecules have been implicated.

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