scholarly journals Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals

2020 ◽  
Author(s):  
Cecilia Peralta ◽  
Diego Herman Sauka ◽  
Antonela Marozzi ◽  
Eleodoro E. Del Valle ◽  
Leopoldo Palma
Keyword(s):  
Bacillus Thuringiensis ◽  
Parasporal Crystals ◽  
Distinct Morphology

Characterization of Bacillus thuringiensis parasporal crystals using laser speckle technique: effect of crystal concentration and dimension

2015 ◽  
Vol 54 (12) ◽  
pp. 3725 ◽  
Author(s):  
Rana Nassif ◽  
Christelle Abou Nader ◽  
Jihane Rahbany ◽  
Fabrice Pellen ◽  
Dominique Salameh ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Laser Speckle ◽  
Technique Effect ◽  
Parasporal Crystals

Parasporal crystals produced by oligosporogenous mutants of Bacillus thuringiensis (Spo− Cr+)

1980 ◽  
Vol 26 (4) ◽  
pp. 486-491 ◽  
Author(s):  
Donovan E. Johnson ◽  
Debra M. Niezgodski ◽  
George M. Twaddle
Keyword(s):  
Bacillus Thuringiensis ◽  
Inclusion Bodies ◽  
Insect Cells ◽  
Crystalline Inclusion ◽  
Wild Type ◽  
In Vitro Technique ◽  
Mutant Strains ◽  
Embedded Particles ◽  
Parasporal Crystals

Six oligosporogenic (Spo−) mutant strains of Bacillus thuringiensis were selected from survivors of treatment with N-methyl-N′-nitro-N-nitrosoguanidine. Each strain was blocked at or before stage II of spore development, but all produced typical bipyramidal-shaped crystalline inclusion bodies. Toxicity of the parasporal endotoxin isolated from the mutant strains was assayed by an in vitro technique using cultured insect cells, and was comparable with that of normal wild-type parasporal protein. Multiple parasporal inclusion bodies per cell were often produced, and smaller embedded particles were numerous and distinct.

Antimicrobial activity of different proteins and their fragments from Bacillus thuringiensis parasporal crystals against clostridia and archaea

Anaerobe ◽  
2007 ◽  
Vol 13 (1) ◽  
pp. 6-13 ◽  
Author(s):  
Tatyana G. Yudina ◽  
Andrei L. Brioukhanov ◽  
Igor A. Zalunin ◽  
Ludmila P. Revina ◽  
Andrei I. Shestakov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Thuringiensis ◽  
Parasporal Crystals

scholarly journals Evaluating the Insecticidal Genes and Their Expressed Products in Bacillus thuringiensis Strains by Combining PCR with Mass Spectrometry

2008 ◽  
Vol 74 (21) ◽  
pp. 6811-6813 ◽  
Author(s):  
Yunjun Sun ◽  
Zujiao Fu ◽  
Xuezhi Ding ◽  
Liqiu Xia
Keyword(s):  
Mass Spectrometry ◽  
Bacillus Thuringiensis ◽  
Genomic Dna ◽  
Wild Type ◽  
Complementary Method ◽  
Protein Levels ◽  
Parasporal Crystals

ABSTRACT By a combination of PCR and mass spectrometry, a total of five cry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab, and cry1Ia) were detected in genomic DNA from the wild-type Bacillus thuringiensis strain 4.0718, and three protoxins (Cry1Aa, Cry1Ac, and Cry2Aa) were identified in the strain's parasporal crystals. These results indicated that this complementary method may be useful in evaluating B. thuringiensis strains at both the gene and protein levels.

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