Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid

Spathoglottis plicata is an attractive, easy-to-grow, and floriferous terrestrial orchid that has become the most important horticulturally cultivated Spathoglottis species in Southeast Asia. The present research was conducted to study the asymbiotic seed germination and seedling development of this orchid under the influence of various photoperiod and medium treatments. Seeds from 28-day-old capsule were sown on five culture media, including half-strength Murashige and Skoog medium (1/2 MS), Orchid Seed Sowing Medium (OSSM), BM-1 Terrestrial Orchid Medium (BM-1), Vacin and Went Modified Orchid Medium (VW), and Knudson C Orchid Medium (KC), and incubated under 0/24-hour or 16/8-hour light/dark photoperiod. Seed germination occurred in all photoperiods and media tested but at different paces. Seeds subjected to total darkness germinated more rapidly in the first 3 weeks than those subjected to light. However, seed germination under light overtook or even exceeded seed germination under dark starting on the fourth week of culture. Seedlings grown on the OSSM and VW media showed the fastest development as they reached the advanced stage (Stage 6) within 11 weeks of culture. Seedlings on the BM-1 medium were the slowest to evolve, as they required more than 16 weeks’ time to complete all the developmental stages. Light-incubated advanced stage seedlings were subcultured on the same medium until leaves and roots were well developed and acclimatized in the greenhouse with 100% survival.

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Improvement of asymbiotic seed germination and seedling development ofCypripedium macranthosSw. with organic additives

Journal of Plant Biotechnology ◽

10.5010/jpb.2016.43.1.138 ◽

2016 ◽

Vol 43 (1) ◽

pp. 138-145 ◽

Cited By ~ 10

Author(s):

Yoon Sun Huh ◽

Joung Kwan Lee ◽

Sang Young Nam ◽

Kee Yoeup Paek ◽

Gang Uk Suh

Keyword(s):

Seed Germination ◽

Seedling Development ◽

Organic Additives ◽

Asymbiotic Seed Germination

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The micorryzal fungi Ceratobasidium sp. and Sebacina vermifera promote seed germination and seedling development of the terrestrial orchid Epidendrum secundum Jacq

South African Journal of Botany ◽

10.1016/j.sajb.2019.06.029 ◽

2019 ◽

Vol 125 ◽

pp. 54-61 ◽

Cited By ~ 3

Author(s):

M.E. Durán-López ◽

R. Caroca-Cáceres ◽

K. Jahreis ◽

M. Narváez-Vera ◽

R. Ansaloni ◽

...

Keyword(s):

Seed Germination ◽

Seedling Development ◽

Terrestrial Orchid ◽

Sebacina Vermifera

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In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

Scientific World ◽

10.3126/sw.v9i9.5518 ◽

1970 ◽

Vol 9 (9) ◽

pp. 50-52 ◽

Cited By ~ 3

Author(s):

Bijaya Pant ◽

Sumitra Shrestha ◽

Shreeti Pradhan

Keyword(s):

Acetic Acid ◽

Seed Germination ◽

Seedling Development ◽

Naphthalene Acetic Acid ◽

Ideal Condition ◽

Ms Medium ◽

Mass Propagation ◽

Asymbiotic Seed Germination ◽

Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

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