The micorryzal fungi Ceratobasidium sp. and Sebacina vermifera promote seed germination and seedling development of the terrestrial orchid Epidendrum secundum Jacq

Spathoglottis plicata is an attractive, easy-to-grow, and floriferous terrestrial orchid that has become the most important horticulturally cultivated Spathoglottis species in Southeast Asia. The present research was conducted to study the asymbiotic seed germination and seedling development of this orchid under the influence of various photoperiod and medium treatments. Seeds from 28-day-old capsule were sown on five culture media, including half-strength Murashige and Skoog medium (1/2 MS), Orchid Seed Sowing Medium (OSSM), BM-1 Terrestrial Orchid Medium (BM-1), Vacin and Went Modified Orchid Medium (VW), and Knudson C Orchid Medium (KC), and incubated under 0/24-hour or 16/8-hour light/dark photoperiod. Seed germination occurred in all photoperiods and media tested but at different paces. Seeds subjected to total darkness germinated more rapidly in the first 3 weeks than those subjected to light. However, seed germination under light overtook or even exceeded seed germination under dark starting on the fourth week of culture. Seedlings grown on the OSSM and VW media showed the fastest development as they reached the advanced stage (Stage 6) within 11 weeks of culture. Seedlings on the BM-1 medium were the slowest to evolve, as they required more than 16 weeks’ time to complete all the developmental stages. Light-incubated advanced stage seedlings were subcultured on the same medium until leaves and roots were well developed and acclimatized in the greenhouse with 100% survival.

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Non-TZF Protein AtC3H59/ZFWD3 Is Involved in Seed Germination, Seedling Development, and Seed Development, Interacting with PPPDE Family Protein Desi1 in Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms22094738 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4738

Author(s):

Hye-Yeon Seok ◽

Hyungjoon Bae ◽

Taehyoung Kim ◽

Syed Muhammad Muntazir Mehdi ◽

Linh Vu Nguyen ◽

...

Keyword(s):

Seed Germination ◽

Seed Development ◽

Zinc Finger ◽

Root Length ◽

Primary Root ◽

Seedling Development ◽

Ccch Zinc Finger ◽

Family Protein ◽

Wd40 Domain ◽

Primary Root Length

Despite increasing reports on the function of CCCH zinc finger proteins in plant development and stress response, the functions and molecular aspects of many non-tandem CCCH zinc finger (non-TZF) proteins remain uncharacterized. AtC3H59/ZFWD3 is an Arabidopsis non-TZF protein and belongs to the ZFWD subfamily harboring a CCCH zinc finger motif and a WD40 domain. In this study, we characterized the biological and molecular functions of AtC3H59, which is subcellularly localized in the nucleus. The seeds of AtC3H59-overexpressing transgenic plants (OXs) germinated faster than those of wild type (WT), whereas atc3h59 mutant seeds germinated slower than WT seeds. AtC3H59 OX seedlings were larger and heavier than WT seedlings, whereas atc3h59 mutant seedlings were smaller and lighter than WT seedlings. Moreover, AtC3H59 OX seedlings had longer primary root length than WT seedlings, whereas atc3h59 mutant seedlings had shorter primary root length than WT seedlings, owing to altered cell division activity in the root meristem. During seed development, AtC3H59 OXs formed larger and heavier seeds than WT. Using yeast two-hybrid screening, we isolated Desi1, a PPPDE family protein, as an interacting partner of AtC3H59. AtC3H59 and Desi1 interacted via their WD40 domain and C-terminal region, respectively, in the nucleus. Taken together, our results indicate that AtC3H59 has pleiotropic effects on seed germination, seedling development, and seed development, and interacts with Desi1 in the nucleus via its entire WD40 domain. To our knowledge, this is the first report to describe the biological functions of the ZFWD protein and Desi1 in Arabidopsis.

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Polyamine oxidase 2 is involved in regulating excess spermidine contents during seed germination and early seedling development in Arabidopsis thaliana

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2019.07.022 ◽

2019 ◽

Vol 516 (4) ◽

pp. 1248-1251

Author(s):

Yoshihiro Takahashi ◽

Tatsuaki Uemura ◽

Yuuta Teshima

Keyword(s):

Arabidopsis Thaliana ◽

Seed Germination ◽

Polyamine Oxidase ◽

Seedling Development ◽

Early Seedling ◽

Early Seedling Development

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Accelerating Seed Germination and Juvenile Growth of Sorghum (Sorghum bicolor L. Moench) to Manage Climate Variability through Hydro-Priming

Atmosphere ◽

10.3390/atmos12040419 ◽

2021 ◽

Vol 12 (4) ◽

pp. 419

Author(s):

Siaka Dembélé ◽

Robert B. Zougmoré ◽

Adama Coulibaly ◽

John P. A. Lamers ◽

Jonathan P. Tetteh

Keyword(s):

Seed Germination ◽

Climate Variability ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Seedling Development ◽

Hot Water ◽

Crop Failure ◽

Simple Method ◽

Early Season

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.

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