Retention behavior of microparticles in gravitational field-flow fractionation (GrFFF): Effect of ionic strength

Talanta ◽  
2015 ◽  
Vol 132 ◽  
pp. 945-953 ◽  
Author(s):  
In Suk Woo ◽  
Euo Chang Jung ◽  
Seungho Lee
Author(s):  
Valentin de Carsalade du pont ◽  
Enrica Alasonati ◽  
Sophie Vaslin-Reimann ◽  
Michel Martin ◽  
Mauricio Hoyos ◽  
...  

In this work we study the parameters which are often modified to optimize the separation in asymmetrical field flow fractionation, and we observe their impact on the retention behavior of the analyte. The aim of the work is to improve our knowledge of the phenomena which govern the behavior of the analytes in the channel and to have a better understanding of the limits of the actual theoretical model in order to improve it. Results illustrate that the ionic strength influences the effect of the cross flow rate on the retention time of the particle. The question of the determination of the channel thickness was also addressed.


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