Serum-free hybridoma culture: ethical, scientific and safety considerations

2006 ◽  
Vol 24 (3) ◽  
pp. 105-108 ◽  
Author(s):  
Megha S. Even ◽  
Chad B. Sandusky ◽  
Neal D. Barnard
Keyword(s):  
Serum Free ◽  
Hybridoma Culture ◽  
Safety Considerations

Continuous hybridoma culture in a low-protein serum-free medium supplemented with liposomes

1995 ◽  
Vol 17 (12) ◽  
pp. 1062-1066 ◽  
Author(s):  
Adèle Martial ◽  
Isabelle Gaillard ◽  
Jean-Marc Engasser ◽  
Annie Marc
Keyword(s):  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Low Protein ◽  
Hybridoma Culture ◽  
Protein Serum

Enhanced production of human monoclonal antibodies by the use of fructose in serum-free hybridoma culture media

1993 ◽  
Vol 13 (3) ◽  
pp. 161-173 ◽  
Author(s):  
Katsumi Mochizuki ◽  
Susumu Sato ◽  
Masatoshi Kato ◽  
Shuichi Hashizume
Keyword(s):  
Monoclonal Antibodies ◽  
Culture Media ◽  
Human Monoclonal Antibodies ◽  
Serum Free ◽  
Enhanced Production ◽  
Hybridoma Culture

Effect of insulin on a serum-free hybridoma culture

1995 ◽  
Vol 47 (2) ◽  
pp. 181-185 ◽  
Author(s):  
Weichang Zhou ◽  
Wei-Shou Hu
Keyword(s):  
Serum Free ◽  
Hybridoma Culture

Exogeneous factors are not necessary in attachment, spreading and polarization of hela cells

1978 ◽  
Vol 36 (2) ◽  
pp. 310-311
Author(s):  
W. Liebrich
Keyword(s):  
Hela Cells ◽  
Calf Serum ◽  
Glass Tube ◽  
Serum Free Medium ◽  
Nacl Solution ◽  
Free Medium ◽  
Minimum Essential Medium ◽  
Serum Free ◽  
All Solutions ◽  
Glass Support

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.

Primary culture of rat ependymal cells in serum-free defined medium

1986 ◽  
Vol 390 (2) ◽  
pp. 199-209 ◽  
Author(s):  
M WEIBEL ◽  
B PETTMANN ◽  
J ARTAULT ◽  
M SENSENBRENNER ◽  
G LABOURDETTE
Keyword(s):  
Primary Culture ◽  
Defined Medium ◽  
Ependymal Cells ◽  
Serum Free

Prognostic significance of serum free radical level in head injury

2005 ◽  
Vol 2 (2) ◽  
pp. 107-109
Author(s):  
A. Mishra ◽  
M.F. Huda ◽  
V.P. Singh ◽  
S. Mohanty ◽  
A. Sodhi
Keyword(s):  
Free Radical ◽  
Head Injury ◽  
Prognostic Significance ◽  
Serum Free
Sign in / Sign up

Export Citation Format

Share Document