Flow Cytometry Panel-Reactive Antibody Screening of Anti-HLA Antibodies in the Waiting List Significantly Reduces the Occurrence of Acute Rejection After Kidney Transplantation

2017 ◽  
Vol 49 (8) ◽  
pp. 1719-1723
Author(s):  
I. Dedinská ◽  
N. Mäčková ◽  
K. Macháleková ◽  
J. Miklušica ◽  
B. Palkoci ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Kidney Transplantation ◽  
Acute Rejection ◽  
Waiting List ◽  
Hla Antibodies ◽  
Antibody Screening ◽  
Panel Reactive Antibody ◽  
Reactive Antibody

Multilaboratory Evaluation of Serum Analysis for HLA Antibody and Crossmatch Reactivity by Lymphocytotoxicity Methods

2003 ◽  
Vol 127 (2) ◽  
pp. 149-156 ◽  
Author(s):  
RenéJ. Duquesnoy ◽  
Marilyn Marrari
Keyword(s):  
Serum Antibody ◽  
Hla Antigens ◽  
Test Results ◽  
Antibody Screening ◽  
Panel Reactive Antibody ◽  
Interlaboratory Variability ◽  
Serum Screening ◽  
Antibody Specificities ◽  
American Society ◽  
Reactive Antibody

Abstract Context.—This report presents results of the serum antibody analysis and crossmatch challenges in the proficiency testing program for histocompatibility testing jointly sponsored by the American Society for Histocompatibility and Immunogenetics and the College of American Pathologists. Objective.—To obtain information about consensus rates among participating laboratories that reported antibody screening and crossmatch results by direct complement-dependent lymphocytotoxicity (CDC) and/or anti-human globulin (AHG)–augmentation methods. Design.—We analyzed responses from approximately 165 laboratories participating in 32 surveys during 1993–2000. Most of the testing was done by CDC methods, but increasing proportions of laboratories are using AHG augmentation of these techniques; almost one half of the serum screenings and crossmatches were done by AHG. Results.—A total of 40 serum specimens were screened to determine the percent panel-reactive antibody (PRA) and identify HLA-specific antibodies. Participants often reported very wide ranges of PRA values. Panel-reactive antibody ranges exceeded 60 percentage points for 16 (40%) of the serum screening results by CDC and for 31 (77%) of the results by AHG. The interlaboratory variability of PRA values suggests that in many laboratories, the CDC or AHG procedures were often too insensitive or overly sensitive. The antibody identification results revealed inconsistent patterns among the participants performing CDC or AHG screening. Most participants reported the same primary antibody specificities by both methods. The consensus levels were generally high for the monospecific sera. On the other hand, there was much less agreement among the participants if the sera reacted with 2 or more HLA antigens. Participants using the more sensitive AHG method reported additional antibody specificities in many specimens, but invariably the consensus levels were rather low. A total of 192 serum-cell combinations were used for the crossmatch challenges. There was considerable interlaboratory variability; 21% of the CDC crossmatches and 36% of AHG crossmatches failed to reach the 90% consensus threshold. Conclusions.—This experience demonstrates considerable inconsistencies in serum screening and crossmatching among laboratories participating in the American Society for Histocompatibility and Immunogenetics/College of American Pathologists surveys. A lack of uniformity in test results may limit the efficient application of these methods in a clinical setting. Standardization of crossmatch and antibody screening techniques is highly desirable.

Effect of panel-reactive antibody positivity on graft rejection before or after kidney transplantation

2004 ◽  
Vol 36 (7) ◽  
pp. 2009-2010 ◽  
Author(s):  
K.W. Lee ◽  
S.J. Kim ◽  
D.S. Lee ◽  
H.H. Lee ◽  
J.W. Joh ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Graft Rejection ◽  
Panel Reactive Antibody ◽  
Antibody Positivity ◽  
Reactive Antibody

Positive pre-transplant flow-panel reactive antibody detected after accelerated acute rejection with negative pre-transplant flow crossmatch

2006 ◽  
Vol 20 (s15) ◽  
pp. 33-37 ◽  
Author(s):  
Masayoshi Miura ◽  
Kanako C Kubota ◽  
Tomoo Itoh ◽  
Takenori Ono ◽  
Ken Morita ◽  
...  
Keyword(s):  
Acute Rejection ◽  
Panel Reactive Antibody ◽  
Reactive Antibody

Panel reactive antibody positivity and associated HLA antibodies in Turkish renal transplant candidates

2004 ◽  
Vol 12 (2) ◽  
pp. 185-188 ◽  
Author(s):  
Fatma Nurhan Ozdemir ◽  
Siren Sezer ◽  
Ali Akcay ◽  
Zubeyde Arat ◽  
Munire Turan ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Hla Antibodies ◽  
Panel Reactive Antibody ◽  
Antibody Positivity ◽  
Transplant Candidates ◽  
Reactive Antibody

scholarly journals PRO – AND ANTI-INFLAMMATORY CYTOKINES ARE EARLY MARKERS OF ACUTE REJECTION OF THE TRANSPLANTED KIDNEY

2018 ◽  
Vol 25 (3) ◽  
pp. 119-124
Author(s):  
T. V. FEDORENKO ◽  
N. V. KOLESNIKOVA ◽  
E. F. FILIPPOV
Keyword(s):  
Renal Failure ◽  
Kidney Transplantation ◽  
Acute Rejection ◽  
Inflammatory Cytokines ◽  
Post Transplantation ◽  
Diagnostic Significance ◽  
Hla Antibodies ◽  
Anti Inflammatory ◽  
Xmap Technology

Aim. Determination of diagnostic significance of pro − and anti-inflammatory cytokines in early prognosis of posttransplant renal failure in patients with chronic renal disease.Materials and methods. In the peripheral blood of patients with chronic kidney disease 6 hours before kidney transplantation, multiplex analysis using Simplex ProcartaPlex panel (Bioscience, USA) and xMAP technology (principle of flow cytometry) was used to estimate the content of 10 cytokines: 7 proinflammatory (IL-1b, IL-6, IL-12p70, IL-27, IL-17A, IL-18) and 3 antiinflammatory (IL-1RA, IL-4, IL-13). Identification of HLA-antibodies was carried out with the help of multiplex immunological analysis, using test systems (Gen-Prob, USA), flow analyzer Luminex 200 xMAP technology (bimolecular reactions on the surface of microspheres).Results. The limited diagnostic significance of HLA-antibodies is due to the fact that their detection in the posttransplantation period can be either in the development of acute graft rejection, or in the favorable course of the period after the operation. Meanwhile, the determination of a number of blood cytokines before kidney transplantation allows predicting post-transplantation rejection. In particular, certain criteria favorable course of the period after kidney transplantation by absence of HLA antibodies in patients with chronic renal failure can be considered as the initial (within 6 hours of transplantation) low levels of IL1β, IL6, IL17а. Prognostically the increase in the blood levels of proinflammatory cytokines − IL6, IL17a and anti-inflammatory IL1-RA is a significant marker of acute rejection of a transplanted kidney. Along with this, it is important to note that the appearance of HLA antibodies in patients with a favorable course of the post-transplantation period is associated with an initially elevated level of proinflammatory cytokines such as Il1ß and IL6.Conclusion. Diagnostic value of the evaluated cytokines at the pre-transplant kidney patients determines the feasibility of the inclusion of evaluation of the serum concentration of IL1β, IL6, IL17a, IL1-RA in the programme of pre-transplant laboratory tests .

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