scholarly journals Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction

2014 ◽  
Vol 171 (1-2) ◽  
pp. 182-188 ◽  
Author(s):  
F.S. Campos ◽  
A.C. Franco ◽  
M.T. Oliveira ◽  
R. Firpo ◽  
G. Strelczuk ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bovine Herpesvirus ◽  
Trigeminal Ganglia ◽  
Infected Cattle ◽  
Chain Reaction ◽  
Bovine Herpesvirus 4 ◽  
Polymerase Chain ◽  
Herpesvirus 4

scholarly journals Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1, Bovine Herpesvirus 2, and Bovine Herpesvirus 4 Infections

2002 ◽  
Vol 14 (4) ◽  
pp. 353-356 ◽  
Author(s):  
Luciana De-Giuli ◽  
Simone Magnino ◽  
Pier Giorgio Vigo ◽  
Iris Labalestra ◽  
Massimo Fabbi
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Analysis ◽  
Simultaneous Detection ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Bovine Herpesvirus 1 ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Polymerase Chain ◽  
Herpesvirus 4

A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

scholarly journals Studies on the molecular biological peculiarities of bovine herpesvirus 4

2020 ◽  
Vol 23 (4) ◽  
pp. 411-423
Author(s):  
R. Peshev
Keyword(s):  
Phylogenetic Tree ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Fragment Analysis ◽  
Biological Features ◽  
Bovine Herpesvirus 4 ◽  
European Strain ◽  
American Strain ◽  
Polymerase Chain ◽  
Herpesvirus 4

Studies on the molecular biological features of bovine herpesvirus 4 (BHV 4) strains isolated in Bulgaria have been conducted. Two types of polymerase chain reaction have been developed and applied to confirm the gB and TK genes. A restrictase fragment analysis was performed using various types of restrictase enzymes. The tested Bulgarian strains differed in their restrictase genomic profile from the reference European strain Movar 33/63 and from the American strain DN 599, and were clearly different each from the other. No clear relationship has been established between the restrictase enzyme profiles and the tropism of the isolated viruses. Sequencing of isolated ВHV 4 strains showed homo­logy with the reference European strain Movar 33/63. After construction of the phylogenetic tree, three ВHV 4 strains were at one branch of the phylogenetic tree, while two other strains were at the branch of reference Movar 33/63 strain. Applied molecular biology methods can be successfully used for differentiation and detailed genetic characterisation of the isolated BHV 4 strains.

Detection of Brucella species in organs of naturally infected cattle by polymerase chain reaction

1998 ◽  
Vol 142 (19) ◽  
pp. 512-514 ◽  
Author(s):  
P. Gallien ◽  
C. Dorn ◽  
G. Alban ◽  
C. Staak ◽  
D. Protz
Keyword(s):  
Polymerase Chain Reaction ◽  
Infected Cattle ◽  
Brucella Species ◽  
Chain Reaction ◽  
Polymerase Chain

Evaluation of the presence of equine viral herpesvirus 1 (EHV-1) and equine viral herpesvirus 4 (EHV-4) DNA in stallion semen using polymerase chain reaction (PCR)

2009 ◽  
Vol 71 (9) ◽  
pp. 1381-1389 ◽  
Author(s):  
Imen Hebia-Fellah ◽  
Anne Léauté ◽  
Francis Fiéni ◽  
Stéphan Zientara ◽  
Berthe-Marie Imbert-Marcille ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Herpesvirus 1 ◽  
Polymerase Chain ◽  
Herpesvirus 4

scholarly journals Quantitation of latent varicella-zoster virus DNA in human trigeminal ganglia by polymerase chain reaction.

1993 ◽  
Vol 67 (4) ◽  
pp. 2381-2384 ◽  
Author(s):  
R Mahalingam ◽  
M Wellish ◽  
D Lederer ◽  
B Forghani ◽  
R Cohrs ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Varicella Zoster Virus ◽  
Trigeminal Ganglia ◽  
Chain Reaction ◽  
Varicella Zoster ◽  
Polymerase Chain
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