scholarly journals Tbx2 misexpression impairs deployment of second heart field derived progenitor cells to the arterial pole of the embryonic heart

2009 ◽  
Vol 333 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Laurent Dupays ◽  
Surendra Kotecha ◽  
Brigitt Angst ◽  
Timothy J. Mohun
Keyword(s):  
Progenitor Cells ◽  
Embryonic Heart ◽  
Second Heart Field ◽  
Heart Field

Cardiac embryogenesis

ESC CardioMed ◽  
2018 ◽  
pp. 33-36
Author(s):  
Robert G. Kelly
Keyword(s):  
Progenitor Cells ◽  
Progenitor Cell ◽  
Congenital Heart ◽  
Heart Defects ◽  
Embryonic Heart ◽  
Heart Tube ◽  
Second Heart Field ◽  
Heart Field ◽  
Cell Niche ◽  
The Right

The embryonic heart forms in anterior lateral splanchnic mesoderm and is derived from Mesp1-expressing progenitor cells. During embryonic folding, the earliest differentiating progenitor cells form the linear heart tube in the ventral midline. The heart tube extends in length and loops to the right as new myocardium is progressively added at the venous and arterial poles from multipotent second heart field cardiovascular progenitor cells in contiguous pharyngeal mesoderm. While the linear heart tube gives rise to the left ventricle, the right ventricle, outflow tract, and a large part of atrial myocardium are derived from the second heart field. Progressive myocardial differentiation is controlled by intercellular signals within the progenitor cell niche. The embryonic heart is the template for septation and growth of the four-chambered definitive heart and defects in progenitor cell deployment result in a spectrum of common forms of congenital heart defects.

Cardiac embryogenesis

ESC CardioMed ◽  
2018 ◽  
pp. 33-36
Author(s):  
Robert G. Kelly
Keyword(s):  
Progenitor Cells ◽  
Progenitor Cell ◽  
Congenital Heart ◽  
Heart Defects ◽  
Embryonic Heart ◽  
Heart Tube ◽  
Second Heart Field ◽  
Heart Field ◽  
Cell Niche ◽  
The Right

The embryonic heart forms in anterior lateral splanchnic mesoderm and is derived from Mesp1-expressing progenitor cells. During embryonic folding, the earliest differentiating progenitor cells form the linear heart tube in the ventral midline. The heart tube extends in length and loops to the right as new myocardium is progressively added at the venous and arterial poles from multipotent second heart field cardiovascular progenitor cells in contiguous pharyngeal mesoderm. While the linear heart tube gives rise to the left ventricle, the right ventricle, outflow tract, and a large part of atrial myocardium are derived from the second heart field. Progressive myocardial differentiation is controlled by intercellular signals within the progenitor cell niche. The embryonic heart is the template for septation and growth of the four-chambered definitive heart and defects in progenitor cell deployment result in a spectrum of common forms of congenital heart defects.

Genetic Fate Mapping Identifies Second Heart Field Epicardial Progenitor Cells as a Source of Adipocytes in Arrhythmogenic Right Ventricular Cardiomyopathy

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Raffaella Lombardi
Keyword(s):  
Right Ventricular ◽  
Progenitor Cells ◽  
Arrhythmogenic Right Ventricular Cardiomyopathy ◽  
Fate Mapping ◽  
Ventricular Cardiomyopathy ◽  
Second Heart Field ◽  
Heart Field

Abstract 473 Raffaella Lombardi, Jinjiag Dong, Gabriela Rodriguez, Achim Bell, Univ of Texas Hlth Sci Cnt, Houston, TX; Tack Ki Leung, Montreal Heart Inst, Université de Montréal, Montreal, Quebec, QC, Canada; Robert J Schwartz, Inst of Biotechnology, Texas A&M University, Houston, TX; James T Willerson, Univ of Texas Hlth Sci Cnt, Houston, TX; Ramon Brugada, Montreal Heart Inst, Université de Montréal, Houston, TX, Canada; Ali J Marian, Univ of Texas Hlth Sci Cnt, Houston, TX Raffaella Lombardi, 2008 Finalist and Presenting Author

scholarly journals Properties of Cardiac Progenitor Cells in the Second Heart Field

2016 ◽  
pp. 177-182
Author(s):  
Alexandre Francou ◽  
Robert G. Kelly
Keyword(s):  
Progenitor Cells ◽  
Cardiac Progenitor Cells ◽  
Second Heart Field ◽  
Heart Field

scholarly journals Investigation of T-box gene function in patterning second heart field progenitor cells

2018 ◽  
Vol 10 (2) ◽  
pp. 257
Author(s):  
C. Thellier ◽  
C. De Bono ◽  
S. Zaffran ◽  
M. Theveniau-Ruissy ◽  
R. Kelly
Keyword(s):  
Progenitor Cells ◽  
Gene Function ◽  
T Box ◽  
Second Heart Field ◽  
Heart Field

scholarly journals Second heart field cardiac progenitor cells in the early mouse embryo

2013 ◽  
Vol 1833 (4) ◽  
pp. 795-798 ◽  
Author(s):  
Alexandre Francou ◽  
Edouard Saint-Michel ◽  
Karim Mesbah ◽  
Magali Théveniau-Ruissy ◽  
M. Sameer Rana ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Mouse Embryo ◽  
Cardiac Progenitor Cells ◽  
Early Mouse Embryo ◽  
Second Heart Field ◽  
Heart Field ◽  
Early Mouse

Abstract 473: Genetic Fate Mapping Identifies Second Heart Field Epicardial Progenitor Cells as a Source of Adipocytes in Arrhythmogenic Right Ventricular Cardiomyopathy

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Raffaella Lombardi ◽  
Jinjiag Dong ◽  
Gabriela Rodriguez ◽  
Achim Bell ◽  
Tack Ki Leung ◽  
...  
Keyword(s):  
Right Ventricular ◽  
Progenitor Cells ◽  
Cardiac Myocytes ◽  
Arrhythmogenic Right Ventricular Cardiomyopathy ◽  
Yellow Fluorescent Protein ◽  
Cardiac Progenitor Cells ◽  
Cardiac Cell ◽  
Ventricular Cardiomyopathy ◽  
Second Heart Field ◽  
Heart Field

The pathological hallmark of arrhythmogenic right ventricular cardiomyopathy (ARVC), a genetic disease of desmosomal proteins, is fibro-adipocytic replacement of cardiac myocytes. The cellular origin of adipocytes in ARVC is an enigma. In desmosomal ARVC the impetus has to instigate from cells that express the mutant protein. Cardiac myocytes are the only cells in the heart known to express the desmosomal proteins. Adult myocytes are terminally differentiated, and hence, an unlikely source. In contrast, cardiac progenitor cells can differentiate to different lineages. Thus, we conditionally deleted Dsp, encoding desmoplakin, and concomitantly expressed enhanced yellow fluorescent protein (EYFP) using the Cre-LoxP technique, regulated by Nkx2.5, an early cardiac lineage promoter. We also screened the human hearts with ARVC for co-expression of markers of cardiac cell lineages and adipocytes. We generated Nkx-2.5-Cre:Dsp W/F and Nkx-2.5-Cre:Dsp W/F :R26-EYFP F/F mice, which showed enlarged hearts, depressed cardiac systolic function and patchy areas of fibro-adiposis, particularly at the epicardium. We detected expression of EYFP by immunoblotting (IB) and immunofluorescence (IF) and expression of cardiac progenitor cells and adipocytes markers by IF. A subset of epicardial cells in the Nkx-2.5-Cre:Dsp W/F :R26-EYFP F/F mice co-expressed adipogenic transcription factors C/EBP-α and PPAR-γ along with EYFP and the second heart field markers IsI1 and Mef2c. Likewise, we detected co-expression of C/EBP-α and IsI1 or Mef2C in the fibro-adipocytic regions in human hearts with ARVC. To determine the specific cardiac cell lineage that differentiates to adipocytes, we co-stained sections of human and lineage trace mouse hearts and detected co-expression of SM actin, a marker of smooth muscle cells (SMCs) and C/EBP-α as well as co-expression of cardiac α-actin, a marker of cardiac myocytes, and C/EBP-α but not co-expression of PECAM1, a marker of endothelial cells and C/EBP-α. We conclude excess adipocytes in desmosomal ARVC originate from second heart field epicardial SMCs/myocyte progenitor cells.

scholarly journals Pulmonary endoderm, second heart field and the morphogenesis of distal outflow tract in mouse embryonic heart

2014 ◽  
Vol 56 (4) ◽  
pp. 276-292 ◽  
Author(s):  
Shi Liang ◽  
Hui-Chao Li ◽  
Yun-Xiu Wang ◽  
Shan-Shan Wu ◽  
Yu-Jin Cai ◽  
...  
Keyword(s):  
Embryonic Heart ◽  
Outflow Tract ◽  
Second Heart Field ◽  
Heart Field

Chicken Second Branchial Arch Progenitor Cells Contribute to Heart Musculature in vitro and in vivo

2021 ◽  
pp. 1-12
Author(s):  
Imadeldin Yahya ◽  
Abdulatif Al Haj ◽  
Beate Brand-Saberi ◽  
Gabriela Morosan-Puopolo
Keyword(s):  
Progenitor Cells ◽  
Time Course ◽  
Branchial Arch ◽  
Cell Labeling ◽  
Chicken Embryos ◽  
Second Heart Field ◽  
Chicken Cell ◽  
Heart Field

In the past, the heart muscle was thought to originate from a single source of myocardial progenitor cells. More recently, however, an additional source of myocardial progenitors has been revealed to be the second heart field, and chicken embryos were important for establishing this concept. However, there have been few studies in chicken on how this field contributes to heart muscles in vitro. We have developed an ex vivo experimental system from chicken embryos between stages HH17–20 to investigate how mesodermal progenitors in the second branchial arch (BA2) differentiate into cardiac muscles. Using this method, we presented evidence that the progenitor cells within the BA2 arch differentiated into beating cardiomyocytes in vitro. The beating explant cells were positive for cardiac actin, Nkx2.5, and ventricular myosin heavy chain. In addition, we performed a time course for the expression of second heart field markers (Isl1 and Nkx2.5) in the BA2 from stage HH16 to stage HH21 using in situ hybridization. Accordingly, using EGFP-based cell labeling techniques and quail-chicken cell injection, we demonstrated that mesodermal cells from the BA2 contributed to the outflow tract and ventricular myocardium in vivo. Thus, our findings highlight the cardiogenic potential of chicken BA2 mesodermal cells in vitro and in vivo.

scholarly journals Second heart field and the development of the outflow tract in human embryonic heart

2013 ◽  
Vol 55 (3) ◽  
pp. 359-367 ◽  
Author(s):  
Yan-Ping Yang ◽  
Hai-Rong Li ◽  
Xi-Mei Cao ◽  
Qin-Xue Wang ◽  
Cong-Jin Qiao ◽  
...  
Keyword(s):  
Embryonic Heart ◽  
Outflow Tract ◽  
Second Heart Field ◽  
Heart Field
Sign in / Sign up

Export Citation Format

Share Document