Cytochrome P-450-linked monooxygenase system and drug-induced spectral interactions in human liver microsomes

1974 ◽  
Vol 9 (3) ◽  
pp. 205-216 ◽  
Author(s):  
Olavi Pelkonen ◽  
Eero H. Kaltiala ◽  
Teuvo K.I. Larmi ◽  
Niilo T. Kärki
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Monooxygenase System ◽  
Drug Induced ◽  
Cytochrome P 450

Prediction of Drug-Induced Liver Injury in HepG2 Cells Cultured with Human Liver Microsomes

2015 ◽  
Vol 28 (5) ◽  
pp. 872-885 ◽  
Author(s):  
Jong Min Choi ◽  
Soo Jin Oh ◽  
Ji-Yoon Lee ◽  
Jang Su Jeon ◽  
Chang Seon Ryu ◽  
...  
Keyword(s):  
Liver Injury ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Cells Cultured

scholarly journals Monoclonal antibodies against human cytochrome P-450 recognizing different pregnenolone 16α-carbonitrile-inducible rat cytochromes P-450

1987 ◽  
Vol 248 (1) ◽  
pp. 301-304 ◽  
Author(s):  
T S Barnes ◽  
P M Shaw ◽  
M D Burke ◽  
W T Melvin
Keyword(s):  
Monoclonal Antibodies ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
The Other ◽  
Male Rat ◽  
Rat Liver Microsomes ◽  
Human Cytochrome ◽  
Male Specific ◽  
Cytochrome P 450

Six murine monoclonal antibodies against human hepatic cytochrome P-450 have been raised, using human liver microsomes (microsomal fractions) or semi-purified human cytochrome P-450 as immunogen. All six antibodies recognized the same highly purified of human liver cytochrome P-450 of molecular mass 53 kDa and gave rise to a single band at 53 kDa on immunoblots of human liver microsomes from 11 individuals. The antibodies also recognized proteins at 52 kDa and 54 kDa on immunoblots of control and induced male-rat liver microsomes, showing four different banding patterns. Antibodies HL4 and HP16 recognized a 52 kDa protein that was only weakly expressed in untreated rats and which was strongly induced by pregnenolone 16 alpha-carbonitrile (PCN) but not by phenobarbitone (PB), 3-methylcholanthrene (3MC), isosafrole (ISF), Aroclor 1254 (ARO), clofibrate or imidazole. HP10 and HL5 recognized a constitutive 52 kDa protein that was weakly induced by PCN but not by the other agents and was suppressed by 3MC and ARO. HP3 recognized a 54 kDa protein that was undetectable in control rats but was strongly induced by PB, PCN, ISF and ARO. HL3 appeared to recognize a combination of the proteins recognized by the other antibodies plus a 54 kDa protein that was weakly expressed in control rats. The constitutive proteins recognized were male-specific.

scholarly journals Immunoquantification of epoxide hydrolase and cytochrome P-450 isozymes in fetal and adult human liver microsomes

1985 ◽  
Vol 151 (2) ◽  
pp. 345-350 ◽  
Author(s):  
Thierry CRESTEIL ◽  
Philippe BEAUNE ◽  
Pierre KREMERS ◽  
Chantal CELIER ◽  
F. Peter GUENGERICH ◽  
...  
Keyword(s):  
Human Liver ◽  
Epoxide Hydrolase ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Adult Human ◽  
Cytochrome P 450

EFFECTS OF FLAVONOIDS ISOLATED FROM SCUTELLARIAE RADIX ON CYTOCHROME P-450 ACTIVITIES IN HUMAN LIVER MICROSOMES

2002 ◽  
Vol 65 (5-6) ◽  
pp. 373-381 ◽  
Author(s):  
Ji-Yeon Kim ◽  
Soo Yong Lee ◽  
Dong-Hyun Kim ◽  
Bok-Ryang Kim ◽  
Raekil Park ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Scutellariae Radix ◽  
Cytochrome P 450
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