Estimation of aneuploidy levels for 8, 15, 18, X and Y chromosomes in 97 human sperm samples using fluorescence in situ hybridization

1997 ◽  
Vol 67 (6) ◽  
pp. 1134-1139 ◽  
Author(s):  
Frédéric Morel ◽  
Sylvie Mercier ◽  
Christophe Roux ◽  
Marie Claire Clavequin ◽  
Jean-Luc Bresson
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Sperm ◽  
Y Chromosomes

Fluorescence In situ hybridization to Y chromosomes in decondensed human sperm nuclei

1990 ◽  
Vol 27 (3) ◽  
pp. 200-208 ◽  
Author(s):  
Andrew J. Wyrobek ◽  
Thomas Alhborn ◽  
Rod Balhorn ◽  
Larry Stanker ◽  
Daniel Pinkel
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Sperm ◽  
Y Chromosomes ◽  
Sperm Nuclei

scholarly journals Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2106
Author(s):  
Barbara Kij-Mitka ◽  
Halina Cernohorska ◽  
Svatava Kubickova ◽  
Sylwia Prochowska ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosomes ◽  
Chromosomal Abnormalities ◽  
Molecular Cytogenetics ◽  
Molecular Probes ◽  
Domestic Cat ◽  
Fish Technique ◽  
Y Chromosomes

Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.

Non-disjunction in human sperm: results of fluorescence in situ hybridization studies using two and three probes

1993 ◽  
Vol 2 (11) ◽  
pp. 1929-1936 ◽  
Author(s):  
Briana J. Williams ◽  
Cynthia A. Ballenger ◽  
Henry E. Malter ◽  
Felicia Bishop ◽  
Michael Tucker ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Sperm

Fluorescence in Situ Hybridization Studies on the Sex Chromosome Constitution of Human Sperm

1997 ◽  
Vol 39 (3) ◽  
pp. 217-222 ◽  
Author(s):  
T. Dineen ◽  
A. Nolan ◽  
J. Harrington ◽  
A. Greer ◽  
R. Kennedy ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosome ◽  
Human Sperm ◽  
Chromosome Constitution

Evaluating sex chromosome content of sorted human sperm samples with use of dual-color fluorescence in situ hybridization

1997 ◽  
Vol 176 (6) ◽  
pp. 1172-1180 ◽  
Author(s):  
W.Edward Richards ◽  
Sheila M. Dobin ◽  
Vicki Malone ◽  
Alfred B. Knight ◽  
Thomas J. Kuehl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosome ◽  
Human Sperm ◽  
Dual Color
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