Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron

2002 ◽  
Vol 32 (2) ◽  
pp. 199-205 ◽  
Author(s):  
Gamal A.A. Elbadri ◽  
Paul De Ley ◽  
Lieven Waeyenberge ◽  
Andy Vierstraete ◽  
Maurice Moens ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Dna Sequencing ◽  
Ribosomal Rna ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Internal Transcribed Spacer ◽  
Fragment Length Polymorphism ◽  
Radopholus Similis ◽  
Internal Transcribed Spacer Region ◽  
Restriction Fragment Length

scholarly journals Comparative Sequence Analysis of the tuf and recA Genes and Restriction Fragment Length Polymorphism of the Internal Transcribed Spacer Region Sequences Supply Additional Tools for Discriminating Bifidobacterium lactis from Bifidobacterium animalis

2003 ◽  
Vol 69 (12) ◽  
pp. 7517-7522 ◽  
Author(s):  
Marco Ventura ◽  
Ralf Zink
Keyword(s):  
Sequence Analysis ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Internal Transcribed Spacer Region ◽  
Bifidobacterium Lactis ◽  
Bifidobacterium Animalis ◽  
Restriction Fragment Length

ABSTRACT The relationship between Bifidobacterium lactis and Bifidobacterium animalis was examined by comparative analysis of tuf and recA gene sequences and by restriction fragment length polymorphism analysis of their internal 16S-23S transcribed spacer region sequences. The bifidobacterial strains investigated could be divided into two distinct groups within a single species based on the tuf, recA, and 16S-23S spacer region sequence analysis. Therefore, all strains of B. lactis and B. animalis could be unified as the species B. animalis and divided into two subspecies, Bifidobacterium animalis subsp. lactis and Bifidobacterium animalis subsp. animalis.

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