scholarly journals In vitro biosynthesis of sialosylgalactosylceramide (G7) by mouse brain microsomes.

1976 ◽  
Vol 251 (1) ◽  
pp. 198-203 ◽  
Author(s):  
R K Yu ◽  
S H Lee
Keyword(s):  
Mouse Brain ◽  
Brain Microsomes ◽  
In Vitro Biosynthesis

scholarly journals Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.

1981 ◽  
Vol 256 (23) ◽  
pp. 12306-12309
Author(s):  
J. Pieringer ◽  
S. Keech ◽  
R.A. Pieringer
Keyword(s):  
Mouse Brain ◽  
Brain Microsomes

scholarly journals A VIRUS EFFECT ON THE UPTAKE OF C14 FROM GLUCOSE IN VITRO BY AMINO ACIDS IN MOUSE BRAIN

1951 ◽  
Vol 193 (1) ◽  
pp. 205-217
Author(s):  
MaxE. Rafelson ◽  
RichardJ. Winzler ◽  
HaroldE. Pearson
Keyword(s):  
Amino Acids ◽  
Mouse Brain

In vitro biosynthesis of steroid sulfates by cell-free preparations from tissues of the laying hen

1968 ◽  
Vol 46 (8) ◽  
pp. 749-757 ◽  
Author(s):  
H. R. Raud ◽  
R. Hobkirk
Keyword(s):  
Enzyme System ◽  
Laying Hen ◽  
Product Identification ◽  
Temperature Requirements ◽  
Before And After ◽  
Liver Preparation ◽  
Steroid Sulfate ◽  
In Vitro Biosynthesis ◽  
Estradiol 17Β

The sulfurylation of estrone-6,7-3H, estradiol-17β-6,7-3H, and dehydroisoandrosterone-4-14C by laying hen liver, oviduct, and vaginal preparations was investigated. Purification and product identification included ether and ethyl acetate extraction, paper chromatography, and isotope dilution, before and after hydrolysis with a sulfatase preparation.The esterifying enzymes were found in the 105 000 × g supernatants of the three tissues. The liver preparation was many times more active in steroid sulfate synthesis than the corresponding oviduct or vaginal fractions. Sulfurylation of dehydroisoandrosterone displayed the same cofactor, pH, and temperature requirements as did that of estrone and estradiol-17β. The degree of dehydroisoandrosterone sulfate synthesis was considerably lower than that of the estrogens, however. It is suggested that the laying hen possesses an enzyme system which is more efficient for the sulfurylation of estrogens than of other steroids such as dehydroisoandrosterone.

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