scholarly journals Glucuronosyl diacylglycerol of Pseudomonas diminuta ATCC 11568. In vitro biosynthesis from UDP-glucuronate and diacylglycerol.

1977 ◽  
Vol 252 (12) ◽  
pp. 4391-4394 ◽  
Author(s):  
J M Shaw ◽  
R A Pieringer
Keyword(s):  
Pseudomonas Diminuta ◽  
In Vitro Biosynthesis

In vitro biosynthesis of steroid sulfates by cell-free preparations from tissues of the laying hen

1968 ◽  
Vol 46 (8) ◽  
pp. 749-757 ◽  
Author(s):  
H. R. Raud ◽  
R. Hobkirk
Keyword(s):  
Enzyme System ◽  
Laying Hen ◽  
Product Identification ◽  
Temperature Requirements ◽  
Before And After ◽  
Liver Preparation ◽  
Steroid Sulfate ◽  
In Vitro Biosynthesis ◽  
Estradiol 17Β

The sulfurylation of estrone-6,7-3H, estradiol-17β-6,7-3H, and dehydroisoandrosterone-4-14C by laying hen liver, oviduct, and vaginal preparations was investigated. Purification and product identification included ether and ethyl acetate extraction, paper chromatography, and isotope dilution, before and after hydrolysis with a sulfatase preparation.The esterifying enzymes were found in the 105 000 × g supernatants of the three tissues. The liver preparation was many times more active in steroid sulfate synthesis than the corresponding oviduct or vaginal fractions. Sulfurylation of dehydroisoandrosterone displayed the same cofactor, pH, and temperature requirements as did that of estrone and estradiol-17β. The degree of dehydroisoandrosterone sulfate synthesis was considerably lower than that of the estrogens, however. It is suggested that the laying hen possesses an enzyme system which is more efficient for the sulfurylation of estrogens than of other steroids such as dehydroisoandrosterone.

In vitro biosynthesis, isolation, and activities of nuclear glycoproteins

1994 ◽  
Vol 30 (1) ◽  
pp. 104-108
Author(s):  
O. Kh. Saitmuratova ◽  
V. B. Leont'ev ◽  
Ya. Kh. Turakulov
Keyword(s):  
In Vitro Biosynthesis

In vitro biosynthesis of functional Escherichia coli su3+tyrosine transfer RNA

Biochemistry ◽  
1977 ◽  
Vol 16 (16) ◽  
pp. 3608-3618 ◽  
Author(s):  
Maurille J. Fournier ◽  
Elizabeth Webb ◽  
Shirley Tang
Keyword(s):  
Escherichia Coli ◽  
Transfer Rna ◽  
In Vitro Biosynthesis
Sign in / Sign up

Export Citation Format

Share Document