scholarly journals Thapsigargin causes Ca2+ release and collapse of the membrane potential of Trypanosoma brucei mitochondria in situ and of isolated rat liver mitochondria.

1993 ◽  
Vol 268 (12) ◽  
pp. 8564-8568
Author(s):  
A.E. Vercesi ◽  
S.N. Moreno ◽  
C.F. Bernardes ◽  
A.R. Meinicke ◽  
E.C. Fernandes ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Trypanosoma Brucei ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat

scholarly journals Studies on the efflux of metalloporphyrin from isolated rat liver mitochondria. Effect of respiratory substrates and metabolic inhibitors

1982 ◽  
Vol 202 (1) ◽  
pp. 41-46 ◽  
Author(s):  
P Husby ◽  
I Romslo
Keyword(s):  
Membrane Potential ◽  
Rat Liver ◽  
Incubation Medium ◽  
Energy State ◽  
Liver Mitochondria ◽  
Metabolic Inhibitors ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat

Intramitochondrially synthesized Co-deuteroporphyrin is released to the incubation medium at a rate inversely correlated to the energy state of the mitochondria; i.e. the rate of efflux increases when substrate is depleted, respiration inhibited or the mitochondria are uncoupled. The efflux of Co-deuteroporphyrin from mitochondria remains low as long as the residual membrane potential is above one-third that of maximally energized mitochondria. Globin enhances the efflux of Co-deuteroporphyrin not only from mitochondria depleted of substrates [Husby & Romslo (1980) Biochem. J. 188, 459-465], but also from maximally energized mitochondria. The results provide further evidence for a co-operative mechanism between the mitochondria and their surroundings for the mobilization of metalloporphyrin from mitochondria.

scholarly journals PREPARATION OF ISOLATED RAT LIVER MITOCHONDRIA FOR ELECTRON MICROSCOPY

1970 ◽  
Vol 44 (2) ◽  
pp. 278-289 ◽  
Author(s):  
W. H. Butler ◽  
J. D. Judah
Keyword(s):  
Electron Microscopy ◽  
Comparative Study ◽  
Potassium Permanganate ◽  
Rat Liver ◽  
Osmium Tetroxide ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat

A comparative study of the fixation of isolated rat liver mitochondria was undertaken. If the criterion is adopted that after processing, the mitochondria should resemble as closely as possible rat liver mitochondria in situ, the procedure found to produce such preservation was that of fixation in suspension in veronal-buffered 2% potassium permanganate. Fixation in osmium tetroxide produced variable results, while mitochondria fixed in glutaraldehyde were contracted. We suggest that in cases where fixation procedures modify the morphological appearance of mitochondria, the significance of such changes must be treated with caution.

Interaction of aromatic aldehydes with isolated rat liver mitochondria

1982 ◽  
Vol 31 (11) ◽  
pp. 2025-2029 ◽  
Author(s):  
Charles R. Wolf ◽  
Helen Harmon ◽  
Carol M. Schiller
Keyword(s):  
Rat Liver ◽  
Aromatic Aldehydes ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat
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