Poly(styrene—divinylbenzene) as reversed-phase adsorbent for the high-performance liquid chromatographic analysis of thiochrome derivatives of thiamine and phosphorylated esters

1984 ◽  
Vol 295 ◽  
pp. 486-491 ◽  
Author(s):  
José Bontemps ◽  
Lucien Bettendorff ◽  
Jacques Lombet ◽  
Christian Grandfils ◽  
Guy Dandrifosse ◽  
...  
Keyword(s):  
Chromatographic Analysis ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Styrene Divinylbenzene ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Derivatives Of

Application of "high-performance" liquid chromatography to the study of sphingolipidoses.

1980 ◽  
Vol 26 (10) ◽  
pp. 1499-1503 ◽  
Author(s):  
M D Ullman ◽  
R E Pyeritz ◽  
H W Moser ◽  
D A Wenger ◽  
E H Kolodny
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Plasma Exchange ◽  
Chromatographic Analysis ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Urine Sediment ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract Quantitative high-performance liquid chromatographic analysis of perbenzoylated sphingolipids has been used to study the correlations of body chemistry to clinical phenomena. Plasma sphingolipids were isolated from 32 Gaucher (β-glucosidase deficiency) and six Fabry (α-galactosidase deficiency) patients by solvent partition and chromatographic separation on silicic acid columns. Plasma sphingolipids from a patient undergoing plasma-exchange were separated from interfering lipids with reversed-phase columns. Liquid-chromatographic analysis of sphingolipids provides useful supportive information for diagnoses because affected individuals are shown to possess increased circulating concentrations of the pathognomonic sphingolipid. We also used this technique to monitor sphingolipid concentrations in plasma and urine sediment during plasma exchange of a p atient with Fabry’s disease. Regular plasma exchanges produced and maintained decreased concentrations of sphingolipids in plasma, but near pre-exchange concentrations were observed within days after the therapy was terminated.

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