chromatographic analysis
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2022 ◽  
Vol 8 (4) ◽  
pp. 270-279
Anup K Chakraborty ◽  
Harshita Tripathi ◽  
Sarita Karole ◽  
Kavita R Loksh

is a significant therapeutic plant has a place with family apocynaceae contains in excess of 70 distinct sorts of chemotherapeutic agents and alkaloids which help in treating different illnesses. For the most part, it is known as Vincarosea, Ammocallisrosea and Lochnerarosea. There are numerous or more than 400 alkaloids present in plant, which are used as flavor, agrochemicals, pharmaceuticals, fragrance, ingredients, food addictives, and pesticides. To develop a validated high performance thin layer chromatographic method for the analysis of leaves and flower extracts of Sample solutions were applied onto the plates with automatic TLC sampler Linomat V (Camag, Muttenz, Switzerland) and were controlled by WinCATS software. Plates were developed in 10 x 10cm twin trough glass chamber (Camag, Muttenz, Switzerland). A CAMAG TLC scanner was used for scanning the TLC plates. Pre-coated silica gel aluminium plates 60F254. For vincristine, simultaneous estimation of vincristine was performed by HPTLC on a silica gel plate using toluene-methanol-diethylamine (8.75: 0.75: 0.5, v/v/v) as the mobile phase. The method was validated as per the ICH guidelines. The Rf value was found to be 0.76 for flower and 0.80 for leaves at 250 nm which shows the presence of vincristin in . In this research paper, a validated HPTLC Method has been developed for the analysis of leaves and flower extracts

Separations ◽  
2022 ◽  
Vol 9 (1) ◽  
pp. 15
Oleg V. Rodinkov ◽  
Alexey Y. Pisarev ◽  
Leonid N. Moskvin ◽  
Aleksandra S. Bugaichenko ◽  
Pavel N. Nesterenko

In this study, a novel approach in headspace gas chromatographic analysis using the selective absorption of the gas extractant during concentration of the analytes was developed. The carbon dioxide used as the gas extractant was removed from the sample flow by passing it through a column packed with microdispersed sodium hydroxide granules. The analytical capabilities of the suggested method were illustrated by the determination of aliphatic and aromatic hydrocarbons in water. We established that this method allows the preconcentration of analytes in the gas phase to be increased proportionally to the volume ratios of the gas extractant before and after absorption, while the analyte limits of detection decrease 30-fold. For example, benzene can be detected in water at a concentration of 0.5 μg/L.

2022 ◽  
Vol 11 ◽  
Zu-Nan Tang ◽  
Lei-Hao Hu ◽  
Hui Yuh Soh ◽  
Yao Yu ◽  
Wen-Bo Zhang ◽  

ObjectiveTo evaluate the feasibility and accuracy of mixed reality combined with surgical navigation in oral and maxillofacial tumor surgery.MethodsRetrospective analysis of data of seven patients with oral and maxillofacial tumors who underwent surgery between January 2019 and January 2021 using a combination of mixed reality and surgical navigation. Virtual surgical planning and navigation plan were based on preoperative CT datasets. Through IGT-Link port, mixed reality workstation was synchronized with surgical navigation, and surgical planning data were transferred to the mixed reality workstation. Osteotomy lines were marked with the aid of both surgical navigation and mixed reality images visualized through HoloLens. Frozen section examination was used to ensure negative surgical margins. Postoperative CT datasets were obtained 1 week after the surgery, and chromatographic analysis of virtual osteotomies and actual osteotomies was carried out. Patients received standard oncological postoperative follow-up.ResultsOf the seven patients, four had maxillary tumors and three had mandibular tumors. There were total of 13 osteotomy planes. Mean deviation between the planned osteotomy plane and the actual osteotomy plane was 1.68 ± 0.92 mm; the maximum deviation was 3.46 mm. Chromatographic analysis showed error of ≤3 mm for 80.16% of the points. Mean deviations of maxillary and mandibular osteotomy lines were approximate (1.60 ± 0.93 mm vs. 1.86 ± 0.93 mm). While five patients had benign tumors, two had malignant tumors. Mean deviations of osteotomy lines was comparable between patients with benign and malignant tumors (1.48 ± 0.74 mm vs. 2.18 ± 0.77 mm). Intraoperative frozen pathology confirmed negative resection margins in all cases. No tumor recurrence or complications occurred during mean follow-up of 15.7 months (range, 6-26 months).ConclusionThe combination of mixed reality technology and surgical navigation appears to be feasible, safe, and effective for tumor resection in the oral and maxillofacial region.

2022 ◽  
Shasthree Taduri ◽  
Suvarchala Vankudoth ◽  
Pavani Chirumamilla ◽  
Spoorthi Veera

The study aimed to identify bioactive compounds in <i>Muntingia calabura</i> leaf and root methanolic extracts. The Gas Chromatography and Mass Spectroscopy (GC-MS) technique were used to identify bioactive compounds. GC-MS analysis revealed 38 compounds in the leaf and 15 compounds in the root methanolic extracts of <i>M. calabura</i>. The prime potent compound found in leaf extract is 2-{3-[(E)-2-(1H-indol-3-yl)ethenyl]-1,2,4-oxadiazol-5-yl}phenol with 5.78% peak area and cholest-4-en-6-on-3-ol is found in root extracts, has the highest 63.7% peak area and another potent compound Lupeol has 7.3% peak area. The bioactive compounds identified in <i>M. calabura</i> have antibacterial activity against various bacterial strains such as gram-positive and gram-negative bacteria, which showed the efficacy of <i>in vivo</i> plant extracts. These findings validate the therapeutic potentiality of <i>M. calabura</i> leaf and root samples. Furthermore, these screened potential bioactive compounds can be used effectively for biomedical and therapeutic applications.

2021 ◽  
Vol 31 (2) ◽  
André Luiz Gomes da Silva

Este trabalho teve como objetivo estudar a biologia reprodutiva de Neomarica northiana (Schneev) Sprague (Iridaceae), erva perene que ocorre nas restingas do estado do Rio de Janeiro. A flor desta espécie apresenta três unidades funcionais de polinização denominada “meranthia”. O recurso floral oferecido aos polinizadores é a substância lipídica exsudada na base das tépalas. Análise de cromotografia gasosa das tépalas indicou a presença de limoneno. As flores de N. northiana são polinizadas exclusivamente por abelhas poliléticas de grande porte, Xylocopa sp.. Neomarica northiana é autocompatível. A produção de frutos por polinização natural foi maior do que a produção por polinização manual cruzada. A taxa de fecundidade é de 0,47. Neomarica northiana apresenta crescimento clonal que pode ocorrer de duas formas distintas, por meio do caule rizomatoso e por meio de bulbilhos formados na axila das brácteas.AbstractThe objective of this research was to evaluate the reproductive biology of Neomarica northiana (Iridaceae), a perennial herb that occurs in the Atlantic Coast restingas (humid subtropical broadleaf forest) of the state of Rio de Janeiro. Each flower of this species has three functional pollinating units called “meranthia”. The main floral resource provided to bees by Neomarica northiana is a lipid substance exuded at the base of the tepals. Gas-chromatographic analysis of tepal samples revealed the presence of limonene. Flowers of N. northiana are pollinated exclusively by large bodied species of bees (Xylocopa sp.). Fruit production from natural pollination is higher than from manual pollination. The fecundity rate is 0.47. Neomarica northiana has clonal growth, which may occur by means of the rhizomatous stem, which produces offshoots close to one another; or by means of axillary bulbils. 

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7539
Evgeniy V. Suslov ◽  
Konstantin Y. Ponomarev ◽  
Oxana S. Patrusheva ◽  
Sergey O. Kuranov ◽  
Alina A. Okhina ◽  

A number of new chiral bispidines containing monoterpenoid fragments have been obtained. The bispidines were studied as ligands for Ni-catalyzed addition of diethylzinc to chalcones. The conditions for chromatographic analysis by HPLC-UV were developed, in which the peaks of the enantiomers of all synthesized chiral products were separated, which made it possible to determine the enantiomeric excess of the resulting mixture. It was demonstrated that bispidine-monoterpenoid conjugates can be used as the ligands for diethylzinc addition to chalcone C=C double bond but not as inducers of chirality. Besides products of ethylation, formation of products of formal hydrogenation of the chalcone C=C double bond was observed in all cases. Note, that this formation of hydrogenation products in significant amounts in the presence of such catalytic systems was found for the first time. A tentative scheme explaining the formation of all products was proposed.

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