Castor-bean acid lipase catalysed hydrolysis of triacylglycerols does not involve C-2 → C-1,3 transacylation

1985 ◽  
Vol 23 (12) ◽  
pp. 3047-3048
Author(s):  
T Diez
Keyword(s):  
Castor Bean ◽  
Acid Lipase ◽  
Hydrolysis Of

Castor-bean acid lipase catalysed hydrolysis of triacylglycerols does not involve C-2 → C-1,3 transacylation

1985 ◽  
Vol 24 (12) ◽  
pp. 3047-3048 ◽  
Author(s):  
Tomás A. Diez ◽  
Julio F. Mata-Segreda
Keyword(s):  
Castor Bean ◽  
Acid Lipase ◽  
Hydrolysis Of

Studies on positional specificity of the castor bean acid lipase

Lipids ◽  
1969 ◽  
Vol 4 (4) ◽  
pp. 261-264 ◽  
Author(s):  
Robert L. Ory ◽  
James Kiser ◽  
Paul A. Pradel
Keyword(s):  
Castor Bean ◽  
Acid Lipase ◽  
Positional Specificity

Acid Lipase of Castor Bean Lipid Bodies : Isolation and Characterisation

1997 ◽  
Vol 6 (1) ◽  
pp. 13-18 ◽  
Author(s):  
A. Altaf ◽  
T. V. Ankers ◽  
N. Kaderbhai ◽  
E. I. Mercer ◽  
M. A. Kaderbhai
Keyword(s):  
Castor Bean ◽  
Lipid Bodies ◽  
Acid Lipase

Role of lysosomal acid lipase in the intracellular metabolism of LDL-transported dehydroepiandrosterone-fatty acyl esters

2008 ◽  
Vol 295 (6) ◽  
pp. E1455-E1461 ◽  
Author(s):  
Feng Wang ◽  
Wei Wang ◽  
Kristiina Wähälä ◽  
Herman Adlercreutz ◽  
Elina Ikonen ◽  
...  
Keyword(s):  
Hela Cells ◽  
Ldl Receptor ◽  
Fatty Acyl ◽  
Biologically Active ◽  
Acid Lipase ◽  
Lysosomal Acid ◽  
Lysosomal Acid Lipase ◽  
Cellular Fraction ◽  
Hydrolysis Of

Dehydroepiandrosterone-fatty acyl esters (DHEA-FAE) belong to a unique family of naturally occurring hydrophobic steroid hormone derivatives that are transported in circulating lipoproteins and may act as a source of dehydroepiendrosterone (DHEA) and other biologically active steroid hormones in cells. Here, we studied the metabolic fate of low-density lipoprotein-associated [3H]DHEA-FAE ([3H]DHEA-FAE-LDL) and the possible role of lysosomal acid lipase (LAL) in the hydrolysis of DHEA-FAE in cultured human cells. When HeLa cells were incubated with [3H]DHEA-FAE-LDL, the accumulation of label in the cellular fraction increased with incubation time and could be inhibited by excess unlabeled LDL, suggesting LDL receptor or LDL receptor-related receptor-dependent uptake. During 48 h of chase, decreasing amounts of [3H]DHEA-FAE were found in the cellular fraction, while in the medium increasing amounts of unesterified [3H]DHEA and its two metabolites, [3H]-5α-androstanedione (5α-adione) and [3H]androstenedione (4-adione), appeared. As LDL-cholesteryl ester hydrolysis is dependent on LAL activity, we depleted LAL from HeLa cells using small interfering RNAs and compared the hydrolysis of [3H]DHEA-FAE-LDL and [3H]cholesteryl-FAE-LDL. The results demonstrated a more modest but significant reducing effect on the hydrolysis of [3H]DHEA-FAE compared with [3H]cholesteryl-FAE. Moreover, experiments in LAL-deficient human fibroblasts (Wolman disease patient cells) showed that [3H]DHEA-FAE hydrolysis was not completely dependent on LAL activity. In summary, LDL-transported [3H]DHEA-FAE entered cells via LDL receptor or LDL receptor-related receptor-mediated uptake, followed by intracellular hydrolysis and further metabolism into 5α-adione and 4-adione that were excreted from cells. Although LAL contributed to the deesterification of DHEA-FAE, it was not solely responsible for the hydrolysis.

Hydrolysis of Crystalloid Storage Protein in Castor Bean Seed Endosperm During and Following Germination

1986 ◽  
Vol 37 (12) ◽  
pp. 1879-1886 ◽  
Author(s):  
DAVID J. GIFFORD ◽  
HELENA C. IMESON ◽  
ELA THAKORE ◽  
J. DEREK BEWLEY
Keyword(s):  
Storage Protein ◽  
Castor Bean ◽  
Bean Seed ◽  
Hydrolysis Of

Hydrolysis of vegetable oils catalyzed by lipase extract powder from dormant castor bean seeds

2013 ◽  
Vol 44 ◽  
pp. 452-458 ◽  
Author(s):  
Matheus H.M. Avelar ◽  
Débora M.J. Cassimiro ◽  
Kádima C. Santos ◽  
Rui C.C. Domingues ◽  
Heizir F. de Castro ◽  
...  
Keyword(s):  
Vegetable Oils ◽  
Castor Bean ◽  
Hydrolysis Of

scholarly journals The acid lipase of the castor bean. Properties and substrate specificity

1962 ◽  
Vol 3 (1) ◽  
pp. 99-105
Author(s):  
Robert L. Ory ◽  
Allen J. St. Angelo ◽  
Aaron M. Altschul
Keyword(s):  
Substrate Specificity ◽  
Castor Bean ◽  
Acid Lipase

Use of castor bean seeds as lipase source for hydrolysis of crambe oil

2018 ◽  
Vol 124 ◽  
pp. 254-264 ◽  
Author(s):  
F. Tavares ◽  
J. Petry ◽  
P.R. Sackser ◽  
C.E. Borba ◽  
E.A. Silva
Keyword(s):  
Castor Bean ◽  
Crambe Oil ◽  
Hydrolysis Of

Hydrolysis of vegetable and microbial oils catalyzed by a solid preparation of castor bean lipase

2021 ◽  
pp. 102188
Author(s):  
Sara A. Machado ◽  
Patrícia C.M. Da Rós ◽  
Heizir F. de Castro ◽  
Domingos S. Giordani
Keyword(s):  
Castor Bean ◽  
Hydrolysis Of ◽  
Microbial Oils
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