scholarly journals Lettuce anaphylaxis: Identification and characterization of a lipid transfer protein as the major allergen

2003 ◽  
Vol 111 (2) ◽  
pp. S250
Author(s):  
M. San Miguel-Moncin ◽  
A. Cistero-Bahima ◽  
M. Krail ◽  
S. Scheurer ◽  
E. Enrique ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Major Allergen ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Identification And Characterization

Isoform identification and characterization of Art v 3, the lipid-transfer protein of mugwort pollen

2009 ◽  
Vol 46 (10) ◽  
pp. 1919-1924 ◽  
Author(s):  
Gabriele Gadermaier ◽  
Andrea Harrer ◽  
Tamara Girbl ◽  
Paola Palazzo ◽  
Martin Himly ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Identification And Characterization

Cloning and Characterization of Walnut Lipid Transfer Protein: Not a Major Allergen in Walnut-Allergic U.S. Patients

2006 ◽  
Vol 117 (2) ◽  
pp. S43 ◽  
Author(s):  
S.S. Teuber ◽  
E. Pastorello ◽  
W.R. Peterson ◽  
A.M. Dandekar ◽  
S. Uratsu
Keyword(s):  
Lipid Transfer Protein ◽  
Major Allergen ◽  
Lipid Transfer ◽  
Transfer Protein

Characterization of the major allergen of plum as a lipid transfer protein

2001 ◽  
Vol 756 (1-2) ◽  
pp. 95-103 ◽  
Author(s):  
Elide A Pastorello ◽  
Laura Farioli ◽  
Valerio Pravettoni ◽  
Maria Gabriella Giuffrida ◽  
Claudio Ortolani ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Major Allergen ◽  
Lipid Transfer ◽  
Transfer Protein

scholarly journals Production and Characterization of Monoclonal Antibody against Vit v1: A Grape Allergen Belonging to Lipid Transfer Protein Family

2020 ◽  
Author(s):  
Mitra Hosseinpour ◽  
Kazem Mashayekhi ◽  
Reza Falak ◽  
Sirous Jamalzehi ◽  
Saeed Mohammadian Haftcheshmeh ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Lipid Transfer Protein ◽  
Major Allergen ◽  
Hybridoma Cells ◽  
Diagnostic Purpose ◽  
Lipid Transfer ◽  
Specific Monoclonal Antibody ◽  
Mouse Splenocytes ◽  
Transfer Protein

Allergy to non-specific lipidtransfer protein (nsLTP), the major allergen of grape (Vit v1), is considered as one of the most common fruit allergies in Iran. Therefore, a specific monoclonal antibody (mAb) can be used for the characterization and assessment of. Accordingly, this study aimed to generate and characterize a mAb against Vit v1 with a diagnostic purpose. To this end, Vit v1 allergen (9 kDa) was extracted using a modified Bjorksten extraction method. Natural Vit v1-immunized mouse splenocytes were fused with SP2/0Ag-14 myeloma cells for generating hybridoma cells. Specific antibody-secreting Hybridoma cells were selected using ELISA. Finally, anti-Vit v1 mAb was characterized by western blotting, ELISA, and isotyping methods. In the current study, a 9 kDa (Vit v1) protein was attained fromcrude and fresh juice of grape extracts and the isotype of desired anti-Vit v1 mAb was determined as IgM with k light chain. In addition, The ELISA results demonstrated that anti-Vit v1 mAb was specified against natural Vit v1 in the grape cultivar and related LTP allergens, such as Pla or 3 (p<0.0001). In the present study, a specific mAb was produced for detecting the LTP allergen. This mAb with a confirmed specificity can be utilized for evaluating the LTP allergens and their allergenicity in different grape cultivars.

Purification and characterization of a small (7.3 kDa) putative lipid transfer protein from maize seeds

2003 ◽  
Vol 794 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Mariana S. Castro ◽  
Isabel R. Gerhardt ◽  
Stefania Orrù ◽  
Piero Pucci ◽  
Carlos Bloch
Keyword(s):  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Purification And Characterization ◽  
Transfer Protein ◽  
Maize Seeds

Molecular cloning and characterization of a lipid transfer protein gene (PsLTP1) from Pinus sylvestris (L.)

2018 ◽  
Vol 30 (3) ◽  
pp. 1149-1158
Author(s):  
Nataliya Hrunyk ◽  
Valentina Kovaleva ◽  
Hryhoriy Krynytskyy ◽  
Ivan Gout ◽  
Francisco Amil-Ruiz ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Molecular Cloning ◽  
Protein Gene ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Transfer Protein

Isolation and Partial Characterization of a Novel Lipid Transfer Protein (LTP) and Antifungal Activity of Peptides from Chilli Pepper Seeds

2010 ◽  
Vol 17 (3) ◽  
pp. 311-318 ◽  
Author(s):  
Luana Cruz ◽  
Suzanna Ribeiro ◽  
Andre Carvalho ◽  
Ilka Vasconcelos ◽  
Rosana Rodrigues ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Lipid Transfer Protein ◽  
Partial Characterization ◽  
Lipid Transfer ◽  
Chilli Pepper ◽  
Transfer Protein
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