In vitro production of bovine embryos: Developmental competence is acquired before maturation

1997 ◽  
Vol 47 (5) ◽  
pp. 1061-1075 ◽  
Author(s):  
P. Blondin ◽  
K. Coenen ◽  
L.A. Guilbault ◽  
M.-A. Sirard
Keyword(s):  
Developmental Competence ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

Media and time of oocytes transport influence their developmental competence for in vitro production of bovine embryos

1998 ◽  
Vol 49 (1) ◽  
pp. 299 ◽  
Author(s):  
H. Twagiramungu ◽  
N. Morin ◽  
L.A. Guilbault ◽  
M.A. Sirard ◽  
D. Bousquet
Keyword(s):  
Developmental Competence ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

232 EFFECT OF MEIOTIC ARREST USING BUTYROLACTONE I AND ROSCOVITINE ON IN VITRO PRODUCTION OF BOVINE EMBRYOS

2013 ◽  
Vol 25 (1) ◽  
pp. 264
Author(s):  
R. R. D. Maziero ◽  
M. D. Guastali ◽  
M. J. Sudano ◽  
D. M. Paschoal ◽  
P. N. Guasti ◽  
...  
Keyword(s):  
Linear Models ◽  
Subsequent Development ◽  
Blastocyst Stage ◽  
Developmental Competence ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Negative Effect ◽  
Vitro Production ◽  
Co2 Atmosphere

Butyrolactone I (BL-I) and roscovitine (ROS) are selective inhibitors of the cyclin-dependent kinases, and both have been shown to reversibly inhibit meiotic resumption in cattle oocytes without having a negative effect on subsequent development to the blastocyst stage. The aim of this study was to evaluate the in vitro production of bovine embryos in the presence of BL-I (Enzo Life Sciences International Inc., Plymouth Meeting, PA, USA) and ROS (Sigma-Aldrich, St. Louis, MO, USA). For that, Nellore oocytes were matured in TCM-199 with Earle’s salts + 10% FCS, FSH, and LH, in a 5% CO2 atmosphere. To delay meiosis, the oocytes were maintained for 6 h in medium in the presence of 25 µM BL-I + 6.25 µM ROS. The oocytes were then cultured for 18 h in agent-free medium to resume meiosis, completing 24 h of maturation. After 24 h of maturation (Day 0), oocytes were fertilized in human tubal fluid (HTF, Irvine, New Zealand), in a 5% CO2 atmosphere. Semen was selected through Percoll gradient and the concentration was adjusted to 2 × 106 sperm mL–1. The presumed zygotes were cultured inside a bag in 90-µL droplets of SOFaa + 0.6% BSA + 2.5% FCS in a 5% CO2, 5% O2, and 90% N2 atmosphere until Day 7, when blastocyst rate was evaluated. Five replicates were done. Data were analysed with ANOVA, followed by Tukey’s test using the general linear models procedure (PROC GLM) of SAS, version 9.2 (SAS Institute Inc., Cary, NC, USA). The level of significance adopted was 5%. No statistical differences were observed in blastocyst production rate: control, 33.3 ± 3.74%; BL-I + ROS, 38.0 ± 4.5%. Thus, the presence of BL-I + ROS in the prematuration medium of bovine oocytes did not compromise their subsequent developmental competence. The next steps of the present study are evaluating the kinetics of blastocyst formation and detecting apoptotic cells in situ by TUNEL, which will show that these substances do not compromise embryonic quality.

Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production

Metabolomics ◽  
2016 ◽  
Vol 12 (5) ◽  
Author(s):  
Érika Cristina dos Santos ◽  
Camila Bruna de Lima ◽  
Kelly Annes ◽  
Marcella Pecora Milazzotto
Keyword(s):  
Culture Media ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Noninvasive Characterization ◽  
Vitro Production

Animal factors that influence the in-vitro production of bovine embryos

1999 ◽  
Vol 51 (1) ◽  
pp. 334 ◽  
Author(s):  
H Twagiramungu ◽  
N Morin ◽  
C Brisson ◽  
G Carbonneau ◽  
J Durocher ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

65 Comparison of SexedULTRA-4M™ X and Y chromosome-bearing semen versus conventional semen for the in vitro production of bovine embryos

2022 ◽  
Vol 34 (2) ◽  
pp. 268
Author(s):  
A. Velázquez-Roque ◽  
H. Álvarez-Gallardo ◽  
M. Kjelland ◽  
M. Pérez-Martínez ◽  
F. Villaseñor-González ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

Evaluation of different culture systems on the in vitro production of bovine embryos

2005 ◽  
Vol 63 (4) ◽  
pp. 1131-1141 ◽  
Author(s):  
Daniela Costa Pereira ◽  
Margot Alves Nunes Dode ◽  
Rodolfo Rumpf
Keyword(s):  
Bovine Embryos ◽  
In Vitro Production ◽  
Culture Systems ◽  
Vitro Production

221 EFFECT OF ROCKING ON IN VITRO PRODUCTION OF BOVINE EMBRYOS

2009 ◽  
Vol 21 (1) ◽  
pp. 209
Author(s):  
Y. Serita ◽  
C. Kubota ◽  
T. Kojima
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Developmental Competence ◽  
In Vitro Production ◽  
Humid Atmosphere ◽  
Vitro Fertilization ◽  
Rate Of Development ◽  
Fetal Bovine ◽  
Vitro Production

This study tested whether embryo development yield using in vitro fertilization (IVF) could be improved by rocking cultures. Bovine ovaries were obtained at a slaughterhouse and transported to the laboratory within 6 h. Cumulus–oocyte complexes were collected and 20–25 were transferred in 100-μL drops of TCM-199 containing 10% fetal bovine serum and antibiotics under paraffin oil. Maturation was for 20–24 h at 38.5°C under 5% CO2 and 95% air in a humid atmosphere (IVM). In vitro fertilization was carried out for 6 h using frozen–thawed sperm from a single bull in modified Brackett and Oliphant (BO) medium. Presumptive zygotes were cultured in CR1aa supplemented with 10 mg mL–1 of BSA or 5% FBS for 9 d at 38.5°C under 5% CO2, 5% O2, and 90% N2 in a humid atmosphere (IVC). Rocking was performed to a height of 6 cm every 7 s using a Profile Rocker (New Brunswick Scientific Co., Edison, NJ, USA) in an incubator. Dishes were placed at a 15-cm distance from the fulcrum of the rocker. The conventional method (no rocking) served as a control, and every experiment was replicated 3 times. For Experiment 1, the effect of the period of rocking on developmental competence was examined when COC or zygotes were subjected to rocking for IVM, IVF, or IVC (IVM-move, IVF-move, and IVC-move). There were no significant differences in rates of oocyte maturation, cleavage, and development for IVM-move v. the control, or for rate of development between IVC-move and the control. However, the rate of fertilization for IVF-move was higher than that of the control (88.9 v. 67.5%; P < 0.01), and the rate of development was higher for IVF-move than for the control (39.0 v. 25.7%; P < 0.05). For Experiment 2, the effect of rocking frequency during IVF on development was determined. The IVF cultures were rocked every 7, 3.5, and 1.5 s (IVF-1move, IVF-2move, IVF-3move). The rates of cleavage on IVF-1move, IVF-2move, IVF-3move, and the control were 74.3, 69.8, 68.8, and 60.4%, and the rates of development were 39.0, 48.3, 26.2, and 25.7%, respectively. The rates of development on IVF-1move and IVF-2move were significantly different from the control and IVF-3move (P < 0.01). These results showed that rocking during IVF improved fertilization and embryo yield, and that frequency of rocking affected embryo development.

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