Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production

Metabolomics ◽  
2016 ◽  
Vol 12 (5) ◽  
Author(s):  
Érika Cristina dos Santos ◽  
Camila Bruna de Lima ◽  
Kelly Annes ◽  
Marcella Pecora Milazzotto
Keyword(s):  
Culture Media ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Noninvasive Characterization ◽  
Vitro Production

scholarly journals Microorganisms in cryopreserved semen and culture media used in the in vitro production (IVP) of bovine embryos identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)

2013 ◽  
Vol 80 (4) ◽  
pp. 337-345 ◽  
Author(s):  
Dávila Zampieri ◽  
Vanessa G. Santos ◽  
Patrícia A.C. Braga ◽  
Christina R. Ferreira ◽  
Daniela Ballottin ◽  
...  
Keyword(s):  
Culture Media ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Corrigendum: Factors Influencing in vitro Production of Bovine Embryos: A Review “Published in Asian Journal of Animal and Veterinary Advances 11 (12): 737-756, 2016”

2016 ◽  
Vol 12 (1) ◽  
pp. 50-50
Author(s):  
Ayman H. Abd El-Aziz ◽  
Usama E. Mahrous ◽  
Sherief Z. Kamel ◽  
Ahmed A. Sabek
Keyword(s):  
Bovine Embryos ◽  
In Vitro Production ◽  
Factors Influencing ◽  
Vitro Production

Animal factors that influence the in-vitro production of bovine embryos

1999 ◽  
Vol 51 (1) ◽  
pp. 334 ◽  
Author(s):  
H Twagiramungu ◽  
N Morin ◽  
C Brisson ◽  
G Carbonneau ◽  
J Durocher ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

scholarly journals Isolation and screening for plant growth-promoting (PGP) actinobacteria from Araucaria angustifolia rhizosphere soil

2010 ◽  
Vol 67 (6) ◽  
pp. 743-746 ◽  
Author(s):  
Rafael Leandro Figueiredo de Vasconcellos ◽  
Mylenne Calciolari Pinheiro da Silva ◽  
Carlos Marcelo Ribeiro ◽  
Elke Jurandy Bran Nogueira Cardoso
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Culture Media ◽  
Araucaria Angustifolia ◽  
In Vitro Production ◽  
Soil Ecosystems ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing ◽  
Vitro Production

Actinobacteria are capable of playing several different roles in soil ecosystems. These microorganisms affect other organisms by producing secondary metabolites and are responsible for the degradation of different complex and relatively recalcitrant organic compounds. In our survey of actinobacteria isolated from the rhizosphere of Araucaria angustifolia, five culture media (AI, WYE, YCED, MSSC and LNMS) were compared for their effectiveness in isolating these microorganisms. When summing up all the isolates randomly obtained, we got 103 isolates. After isolation, the phosphate-solubilizing ability and the "in vitro" production of indole-acetic acid and chitinases were evaluated. The AI medium was ineffective for actinobacteria isolation, when it was compared with the other four culture media. Indole-acetic acid and chitinase were produced by respectively 36% and 24% of the strains tested. However, only 2% of the 103 strains presented some phosphate-solubilizing ability. These results demonstrate the biotechnological potential of these microorganisms.

scholarly journals Resveratrol-supplemented holding or re-culture media improves viability of fresh or vitrified-warmed in vitro-derived bovine embryos

2021 ◽  
Vol 10 (14) ◽  
pp. e367101422097
Author(s):  
Arianny Rafaela Neto Silva ◽  
Thaisa Campos Marques ◽  
Elisa Caroline Silva Santos ◽  
Tiago Omar Diesel ◽  
Isabelle Matos Macedo ◽  
...  
Keyword(s):  
Culture Media ◽  
Cell Number ◽  
Time Dependent ◽  
Expansion Rate ◽  
Ros Generation ◽  
Hatching Rate ◽  
Protective Effects ◽  
Bovine Embryos ◽  
In Vitro Production

The effect of resveratrol supplementation on fresh (E1) or vitrified/warmed (E2) in vitro produced bovine embryos was investigated by evaluating the time-dependent response. After in vitro production, resveratrol (0.5 µM) was added to the incubation media and after two incubation periods with or without resveratrol, blastocysts were re-cultured for 24h. The rates of re-expansion, hatching, total cell number (TCN), apoptotic cells (ACN), reactive oxygen species (ROS) and intracellular glutathione (GSH) content were evaluated. For E1, the re-expansion rate differed at 6 and 10h within and between treatments (P<0.05), as did the re-expansion rate after 24h (P<0.01). The hatching rate increased after 10h with resveratrol (P<0.01) with differences within (P<0.05), but not between treatments after 24h of re-cultivation. At E2, hatching rate differed between treatments at 24h (P<0.01), with higher TCN in resveratrol-treated blastocysts after 10h (P<0.01). Resveratrol supplementation reduced ROS generation in E1 and E2 after 10h of incubation and increased GSH content (P<0.01). These results indicate that supplementation of holding re-cultivation medium with resveratrol for treatment of fresh or vitrified/warmed in vitro produced bovine embryos has a positive and time-dependent effect. The reduction of ROS content, the increase of GSH and the anti-apoptotic ability of resveratrol are responsible for its protective effects, allowing an extension of embryo storage time before transfer to recipients.

Characterization of the in vitro production of N-acyl homoserine lactones by cultivable bacteria inhabiting the sponge Suberites domuncula

2016 ◽  
Vol 97 (1) ◽  
pp. 119-127
Author(s):  
Jasnizat Bin Saidin ◽  
Mohd Effendy Abd Wahid ◽  
Gaël Le Pennec
Keyword(s):  
In Vitro Production ◽  
Form Complex ◽  
Suberites Domuncula ◽  
Associated Bacteria ◽  
Homoserine Lactones ◽  
Cultivable Bacteria ◽  
Acyl Homoserine Lactones ◽  
Vitro Production

Sponges together with associated bacteria form complex holobionts governed by various relationships established between the partners. Molecules of communication may participate in the homeostasis of these biological associations. Bacteria produce N-acyl homoserine lactones to communicate. In the present study, we characterized 102 cultivable bacteria isolated from the sponge Suberites domuncula at the genus level and described their production of AHLs during 96 h of culture in a Marine Broth medium. The presence of AHLs was monitored using the Escherichia coli pSB406 reporter strain. Sixty-seven bacteria were AHLs positive. Among them, 46 were related to the Gammaproteobacteria, eight to the Alphaproteobacteria, seven to the Firmicutes and six to the Flavobacteria. The kinetic production of AHLs was determined and compared with a reference bacterium, Pseudomonas aeruginosa PAO1 (pDA224). We established three profiles of AHLs production with no definitive pattern related to an order, a family or a genus of bacteria.

65 Comparison of SexedULTRA-4M™ X and Y chromosome-bearing semen versus conventional semen for the in vitro production of bovine embryos

2022 ◽  
Vol 34 (2) ◽  
pp. 268
Author(s):  
A. Velázquez-Roque ◽  
H. Álvarez-Gallardo ◽  
M. Kjelland ◽  
M. Pérez-Martínez ◽  
F. Villaseñor-González ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

Evaluation of different culture systems on the in vitro production of bovine embryos

2005 ◽  
Vol 63 (4) ◽  
pp. 1131-1141 ◽  
Author(s):  
Daniela Costa Pereira ◽  
Margot Alves Nunes Dode ◽  
Rodolfo Rumpf
Keyword(s):  
Bovine Embryos ◽  
In Vitro Production ◽  
Culture Systems ◽  
Vitro Production
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